Ammonium-assimilating enzymes and erythromycin formation in Saccharolypospora erythrea.

Flores, María Elena; Sánchez, Sergio

doi:10.2323/jgam.35.203

Cited by 9 publications

(1 citation statement)

References 24 publications

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“…The biosynthesis of most antibiotics is strongly regulated by the nature and concentration of the carbon, nitrogen, and phosphorus source and trace elements (Trilli et al, 1987; Potvin and Peringer, 1994; Flores and Sanchez, 1989). It is reported that high concentrations of an easy metabolizable nutrient would reduce antibiotic titer and postpone its onset through catabolite repression (Martin and Demain, 1980).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Rapeseed Oil Uptake on the Production of Erythromycin and Triketide Lactone by Saccharopolyspora erythraea

Mirjalili

Zormpaidis

Leadlay

et al. 1999

Biotechnology Progress

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Saccharopolyspora erythraea was grown in an oil-based process medium at two different laboratory scales. The initial concentration of rapeseed oil in the medium was shown not to affect the growth, while addition of oil significantly increased erythromycin A production. Increasing the agitation speed at the 2 L scale increased the growth of the culture and the production of erythromycin A but had little effect on the level of oil remaining in the fermentation. Maximum oil utilization of 50% (w/w) was obtained in both 2 and 7 L cultures. Gas chromatography analysis of the process medium showed that there was no accumulation of fatty acids and glycerides during the fermentations. The specific oil utilization by the recombinant strain of S. erythraea was significantly lower compared to the wild-type strain grown at the same scale and initial oil concentration; the recombinant strain also produced lower concentrations of the novel polyketide, triketide lactone.

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