Ammonia stress on a resilient mesophilic anaerobic inoculum: Methane production, microbial community, and putative metabolic pathways

Bühlmann, Christopher H.; Mickan, Bede S.; Jenkins, Sue; Tait, Stephan; Kahandawala, Tharanga K.A.; Bahri, Parisa A.

doi:10.1016/j.biortech.2018.12.012

Cited by 58 publications

(27 citation statements)

References 45 publications

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“…These two phyla have repeatedly been reported to be ubiquitous in nearly all microbial communities involved in CH 4 production (Klocke et al, 2007;Sundberg et al, 2013;Solli et al, 2014), which was attributed to their high metabolic versatility and stability (Garcia et al, 2011;Kampmann et al, 2012). Both are prevalent at the initial stages of AD (Rivière et al, 2009;Buhlmann et al, 2019) and either involved in the metabolization of sugars and amino acids into the intermediary products acetate, H 2 , and CO 2 and thus provide substrates for acetogens and methanogens (Mara and Horan, 2003;Tracy et al, 2012) or in the degradation of VFA and thus prevention of methanogenesis inhibition (Krakat et al, 2011). This coincides with the significantly higher relative abundance of Firmicutes, particularly Clostridiales, in the samples using unsterile soil and sterile DFS (Supplementary Figure S3A).…”

Section: Discussionmentioning

confidence: 93%

Soil-Derived Inocula Enhance Methane Production and Counteract Common Process Failures During Anaerobic Digestion

2020

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Although soil-borne methanogens are known to be highly diverse and adapted to extreme environments, their application as potential (anaerobic) inocula to improve anaerobic digestion has not been investigated until now. The present study aimed at evaluating if soil-derived communities can be beneficial for biogas (methane, CH 4 ) production and endure unfavorable conditions commonly associated with digestion failure. Nine study sites were chosen and tested for suitability as inoculation sources to improve biogas production via in situ measurements (CH 4 fluxes, physical and chemical soil properties, and abundance of methanogens) and during a series of anaerobic digestions with (a) combinations of both sterile or unsterile soil and diluted fermenter sludge, and (b) pH-, acetate-, propionate-, and ammonium-induced disturbance. Amplicon sequencing was performed to assess key microbial communities pivotal for successful biogas production. Four out of nine tested soil inocula exerted sufficient methanogenic activity and repeatedly allowed satisfactory CH 4 /biogas production even under deteriorated conditions. Remarkably, the significantly highest CH 4 production was observed using unsterile soil combined with sterile sludge, which coincided with both a higher relative abundance of methanogens and predicted genes involved in CH 4 metabolism in these variants. Different bacterial and archaeal community patterns depending on the soil/sludge combinations and disturbance variations were established and these patterns significantly impacted CH 4 production. Methanosarcina spp. seemed to play a key role in CH 4 formation and prevailed even under stressed conditions. Overall, the results provided evidence that soil-borne methanogens can be effective in enhancing digestion performance and stability and, thus, harbor vast potential for further exploitation.

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Section: Discussionmentioning

confidence: 93%

Soil-Derived Inocula Enhance Methane Production and Counteract Common Process Failures During Anaerobic Digestion

2020

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“…4A) were applied to annotate the putative function of the genus and species based on current literature (Langille et al, 2013) and the KEGG database (kegg.jp/kegg). The PICRUSt has shown its value in the metabolic function of anaerobic digestion under acute ammonia stress and should generally be used with caution (Buhlmann et al, 2019). The module M00157: F-type ATPase and M00159: V-type ATPase were inhibited at initial phase D1 with PPI dose, compared with the control CK group.…”

Section: Evolution Of Microbial Functionsmentioning

confidence: 99%

“…There were two key pathways from acetate to acetyl-CoA: ack and pta via CH 3 -CO-P i for the Wood-Ljungdahl pathway, or acs "one-step" to acetyl-CoA for beta-oxidation (Hagen et al, 2017). These two pathways could co-occurrence in a bacteria (Treu et al, 2018) or a reactor (Buhlmann et al, 2019), and seems substitutable for each other. On the other side, their differences come along with syntrophic methanogenesis of acetate.…”

Section: Decipher Proton Pump In Anaerobic Digestion Under Ammonia Stmentioning

confidence: 99%

Effect of proton pump inhibitor on microbial community, function, and kinetics in anaerobic digestion with ammonia stress

Zhang

Jaegher

et al. 2021

Bioresource Technology

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“…In this study, the abundances of Clostridium and Terrisporobacter were relatively high, which provided a suitable environment for the smooth progress of the hydrolysis and acidi cation process in the AD system. It has been reported that the vadin BC27 wastewater sludge group produced small molecular fatty acids from fermentable amino acids, cysteine, leucine, methionine, serine, tryptophan, and valine [36] , and was well adapted to ammonia nitrogen concentrations [37] .…”

Section: Microbial Communitymentioning

confidence: 99%

Biogas and volatile fatty acid production during anaerobic digestion of straw, cellulose, and hemicellulose with analysis of microbial communities and functions

Liu

Zuo

Peng

et al. 2020

Preprint

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Background Anaerobic digestion (AD) is a promising method for straw treatment, but the complex composition and structure of straw limit AD efficiency and methane production. The main biodegradable components of straw are cellulose and hemicellulose. Because of the different chemical structures and physicochemical properties, the performance of AD of cellulose and hemicellulose is different, thus it’s also different from that of straw. Research on the similarities and differences of AD of straw, cellulose and hemicellulose is helpful to clarify the law of anaerobic digestion of straw and provide theoretical basis for further improving the efficiency of anaerobic digestion. However, there are very few studies on AD using cellulose and hemicellulose as raw materials. Results Rice straw (RS), cellulose, and hemicellulose were used as raw materials to study biogas production performance and changes in the volatile fatty acids (VFAs). Further, microbial communities and genetic functions were analyzed separately for each material. The biogas production potential of RS, cellulose, and hemicellulose was different, with cumulative biogas production of 620.64, 412.50, and 283.75 mL/g·VS− 1, respectively. The methane content of the biogas produced from cellulose and hemicellulose was approximately 10% higher than that produced from RS after the methane content stabilized. Biogas production and the methane content of RS stabilized more quickly than that of cellulose and hemicellulose. The accumulation of VFAs occurred in the early stage of anaerobic digestion in all the three materials, and the main volatile fatty acid component of RS was acetic acid, whereas that of cellulose and hemicellulose was propionic acid. The cumulative amount of VFAs in both cellulose and hemicellulose was relatively higher than that in RS, and the accumulation time was 12 and 14 days longer, respectively. When anaerobic digestion progressed to a stable stage, Clostridium was the dominant bacterial genus in all three AD systems, and the abundance of Ruminofilibacter was higher during anaerobic digestion of RS. Genetically, AD of all the three materials proceeded mainly via aceticlastic methanogenesis, with similar functional components. Conclusion The biogas and VFAs production during AD of RS, cellulose, and hemicellulose showed marked differences. But when the AD progressed to the stable stage, there was no significant difference in microbial community and genetic function. Specifically, the biogas production potential of cellulose and hemicellulose was greater than that of RS. The accumulation of VFAs in the three AD systems occurred in the early stages. The main component of VFA that accumulated in RS was acetic acid, while the major component of VFAs accumulated in cellulose and hemicellulose digestions was propionic acid. At the stable stage, Clostridium was the dominant bacterial genus in all three AD systems. The AD of all the three materials proceeded mainly via aceticlastic methanogenesis, with similar components of gene functions.

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Ammonia stress on a resilient mesophilic anaerobic inoculum: Methane production, microbial community, and putative metabolic pathways

Cited by 58 publications

References 45 publications

Soil-Derived Inocula Enhance Methane Production and Counteract Common Process Failures During Anaerobic Digestion

Soil-Derived Inocula Enhance Methane Production and Counteract Common Process Failures During Anaerobic Digestion

Effect of proton pump inhibitor on microbial community, function, and kinetics in anaerobic digestion with ammonia stress

Biogas and volatile fatty acid production during anaerobic digestion of straw, cellulose, and hemicellulose with analysis of microbial communities and functions

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