Ammonia regulates chicken tracheal cell necroptosis via the LncRNA-107053293/MiR-148a-3p/FAF1 axis

Wang, Wei; Shi, Qunxiang; Wang, Shengchen; Zhang, Hongfu; Xu, Shiwen

doi:10.1016/j.jhazmat.2019.121626

Cited by 81 publications

(33 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HSPs can increase the stress capacity of cells and strengthen organisms' resistance to stimuli. In our present study, we found that TCR γ dysfunction significantly increased the expression of HSP70 and HSP90, which indicated that HSPs play potential protective role in TCR γ dysfunction‐dependent damage (Wang, Shi, Wang, Zhang, & Xu, ).…”

Section: Discussionsupporting

confidence: 53%

Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ

Yang

Gong

Cai

et al. 2020

Journal of Fish Diseases

View full text Add to dashboard Cite

Chlorpyrifos is a poisonous pesticide that is highly toxic to fish and aquatic organisms. However, there are fewer reports about how chlorpyrifos influences the redox balance of immune cells. Herein, the head kidney tissue treated with chlorpyrifos to do transcriptome analysis and TCR γ was screened out. Subsequently, we established TCR γ knockdown and overexpression carp head kidney lymphocyte models, respectively, by using RNA interference and pcDNA3.1. Real‐time PCR, fluorescent staining, oxidation and antioxidant kit were used to detect the related factors. We found that TCR γ knockdown significantly increased the mRNA expression of HSP70 and HSP90 and decreased the mRNA expression of SOD and CAT. Meanwhile, TCR γ knockdown reduced the activities of GSH, GSG‐PX, T‐AOC, CAT and SOD and increased the content of MDA and H2O2 and activities of iNOS. Adverse results were obtained in TCR γ overexpression group. Additionally, TCR γ knockdown significantly increased the mRNA expression of IFN‐γ, IL‐1β, IL‐8, IL‐10, Nrf2 and NF‐κB, but relieved TCR γ overexpression, in which the process of inflammation was activated. Our results reported here indicated that chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ, and HSPs play potential protective role in entire process.

show abstract

Section: Discussionsupporting

confidence: 53%

Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ

Yang

Gong

Cai

et al. 2020

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

“…Ammonia is the most prominent toxic gas in poultry houses. It originates from the breakdown of undigested protein and uric acid in the litter [20] and adversely affects the health [21,22] and growth performance [7,8,23,24] of broilers. In this experiment, a decrease in growth performance was observed in the 25 ppm ammonia group and the 35 ppm ammonia group.…”

Section: The Effect Of Ammonia On Growth Performance Of Broiler Chickensmentioning

confidence: 99%

Effects of Ammonia on Gut Microbiota and Growth Performance of Broiler Chickens

Zhou

Liu

Wang

et al. 2021

Animals

View full text Add to dashboard Cite

In order to investigate the influence of ammonia on broiler intestinal microflora and growth performance of broiler chickens, 288 21-day-old male Arbor Acres broilers with a similar weight were randomly divided into four groups with different NH3 levels: 0 ppm, 15 ppm, 25 ppm, and 35 ppm. The growth performance of each group was recorded and analyzed. Additionally, 16s rRNA sequencing was performed on the cecal contents of the 0 ppm group and the 35 ppm group broilers. The results showed the following: a decrease in growth performance in broilers was observed after 35 ppm ammonia exposure for 7 days and 25 ppm ammonia exposure for 14 days. At phylum level, the relative abundance of Proteobacteria phylum was increased after 35 ppm ammonia exposure. At genus level, ammonia increased the relative abundance of Escherichia–Shigella and decreased the relative abundance of Butyricicoccus, Parasutterella, Lachnospiraceae_UCG-010, Ruminococcaceae_UCG-013 and Ruminococcaceae_UCG-004. Negative correlation between Escherichia–Shigella and growth performance, and positive correlation between bacteria genera (including Butyricicoccus, Parasutterella, Lachnospiraceae_UCG-010, Ruminococcaceae_UCG-013 and Ruminococcaceae_UCG-004) and growth performance was observed. In conclusion, ammonia exposure caused changes in the structure of cecal microflora, and several species were either positively or negatively correlated with growth performance. These findings will help enhance our understanding of the possible mechanism by which ammonia affect the growth of broilers.

show abstract

“…The ileum tissue sample (area, 0.25 cm 2 ) was placed in a 2.5% glutaraldehyde solution. An S-3400N (Hitachi) scanning electron microscope (SEM) was used to observe the samples after gold plating (E-1010; Hitachi) by standard methods (W. Wang, Shi, Wang, Zhang, & Xu, 2020). Transmission electron microscopy (TEM-1200ES; Japan) was used to observe the sample incubated with lead citrate (Liu et al, 2020).…”

Section: Histomorphology Observationmentioning

confidence: 99%

Selenium deficiency induced necroptosis, Th1/Th2 imbalance, and inflammatory responses in swine ileum

Zhang

Bao

et al. 2020

Journal Cellular Physiology

View full text Add to dashboard Cite

Selenium (Se) deficiency has a significant impact on the swine breeding industry by inducing digestive system damage and diarrhea. However, the molecular mechanism remains unclear. Our objectives were to investigate if different amounts of necroptosis, inflammatory responses, and T helper cell 1/T helper cell 2 (Th1/Th2) imbalances were induced by Se deficiency in intestinal porcine jejunal epithelial cells (IPEC-J2) and swine ileum tissue. Therefore, Se-deficient models were successfully established both in vitro and in vivo. In the current study, the cell morphological observation results showed that Se deficiency seriously affected the growth and differentiation of IPEC-J2 cells. Moreover, the necroptosis staining and histomorphology observation results showed that the number of necroptotic cells increased significantly, and the ileal tissue exhibited abnormal structures, including necroptotic features and inflammatory cell infiltration, in the Se-deficient group. Furthermore, Se deficiency resulted in accelerated cell necroptosis by increasing (p < .05) the expression of genes related to the tumor necrosis factor-α pathway at both the protein and messenger RNA (mRNA) levels compared to the control group. Moreover, the relative mRNA and protein expression of the inflammatory genes and their responses to dietary Se deficiency were consistent with the resultant Th1/Th2 imbalances in vitro and in vivo. Taken together, the results suggested that Se deficiency caused necroptosis, inflammatory responses, and abnormal expression of cytokines in swine ileum tissue. These findings might help us to explain the damage induced by Se deficiency to the digestive system of swine.

show abstract

Ammonia regulates chicken tracheal cell necroptosis via the LncRNA-107053293/MiR-148a-3p/FAF1 axis

Cited by 81 publications

References 46 publications

Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ

Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ

Effects of Ammonia on Gut Microbiota and Growth Performance of Broiler Chickens

Selenium deficiency induced necroptosis, Th1/Th2 imbalance, and inflammatory responses in swine ileum

Contact Info

Product

Resources

About