Ammonia-Oligotrophic and Diazotrophic Heavy Metal-Resistant Serratia liquefaciens Strains from Pioneer Plants and Mine Tailings

Zelaya-Molina, Lily X.; Hernández-Soto, Luis Mario; Guerra-Camacho, Jairo E; Monterrubio-López, Ricardo; Patiño-Siciliano, Alfredo; Villa‐Tanaca, Lourdes; Hernández‐Rodríguez, César

doi:10.1007/s00248-016-0771-3

Cited by 13 publications

(8 citation statements)

References 136 publications

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“…ZoB14 has been screened for dual P and ZnO solubilization [80]. Next, we showed that S. rubidaea ED1 grew on a N-free solid medium supporting its oligonitrophilic feature (Table 1) as previously reported in Serratia liquefaciens and Serratia marcescens [81,82]. Indeed, oligonitrophilic bacteria have broad substrate specificities that allow them to maintain their structure in low N environments [83].…”

Section: Discussionsupporting

confidence: 74%

Halotolerant Endophytic Bacterium Serratia rubidaea ED1 Enhances Phosphate Solubilization and Promotes Seed Germination

et al. 2021

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Quinoa is renowned for its nutritional value and ability to withstand harsh environmental conditions such as salinity. In the present work, we isolated 34 phosphate solubilizing endophytic bacteria associated with the roots of quinoa plants. Based on phosphate solubilization efficiency and biochemical characterization, we selected one isolate named ED1. Ribotyping using partial 16S RNA gene analysis revealed that the selected isolate shares 99.7% identity with Serratia rubidaea. Plant growth promoting (PGP) studies showed that the ED1 strain solubilized complexed forms of phosphate (Ca3(PO4)2). Zinc release from ZnO, Zn3(PO4)2, or ZnCO3 revealed the efficient ZnO solubilization by the ED1 strain. Except for proteases, the strain ED1 produced siderophores, cellulase, ammonia and exhibited oligonitrophilic features. Indole acetic acid (IAA) production was detected with and without the L-tryptophan precursor. Next, we demonstrated that the ED1 strain tolerated 1.5 M NaCl final concentration and exhibited intrinsic resistance to seven antibiotics frequently prescribed for medical use. Moreover, we found that ED1 strain withstood 2 mg/L of Cadmium and 1 mg/L of either Nickel or Copper. Furthermore, we observed that S. rubidaea ED1 stimulated quinoa seeds germination and seedlings growth under salt stress conditions. Lastly, we discuss the advantages versus disadvantages of applying the S. rubidaea ED1 strain as a beneficial agent for salty and/or heavy metals contaminated soils.

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Section: Discussionsupporting

confidence: 74%

Halotolerant Endophytic Bacterium Serratia rubidaea ED1 Enhances Phosphate Solubilization and Promotes Seed Germination

et al. 2021

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“…43,44 In corroboration with our current study, Serratia liquefaciens was identified as the dominant taxon in mine tailings with the capability to heterotrophically fix N 2 , suggesting that these bacteria may thrive in the harsh environments in mine tailings and play an important role in N fixation in tailing ecosystems. 42 N fixation coupled with As(III)-oxidation, however, has not been detected in Serratia spp. previously.…”

Section: ■ Resultsmentioning

confidence: 94%

“…are found over a wide diversity of habitats due to their metabolic versatility. 41,42 Notably, members of Serratia have been frequently identified as heterotrophic diazotrophs. For example, Serratia marcescens was isolated from surface-sterilized roots and stems of four different rice varieties with the capability to fix N 2 .…”

Section: ■ Resultsmentioning

confidence: 99%

Serratia spp. Are Responsible for Nitrogen Fixation Fueled by As(III) Oxidation, a Novel Biogeochemical Process Identified in Mine Tailings

Guo

Häggblom

et al. 2022

Environ. Sci. Technol.

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Biological nitrogen fixation (BNF) has important environmental implications in tailings by providing bioavailable nitrogen to these habitats and sustaining ecosystem functions. Previously, chemolithotrophic diazotrophs that dominate in mine tailings were shown to use reduced sulfur (S) as the electron donor. Tailings often contain high concentrations of As(III) that might function as an alternative electron donor to fuel BNF. Here, we tested this hypothesis and report on BNF fueled by As(III) oxidation as a novel biogeochemical process in addition to BNF fueled by S. Arsenic (As)-dependent BNF was detected in cultures inoculated from As-rich tailing samples derived from the Xikuangshan mining area in China, as suggested by nitrogenase activity assays, quantitative polymerase chain reaction, and 15 N 2 enrichment incubations. As-dependent BNF was also active in eight other As-contaminated tailings and soils, suggesting that the potential for As-dependent BNF may be widespread in As-rich habitats. DNAstable isotope probing identified Serratia spp. as the bacteria responsible for Asdependent BNF. Metagenomic binning indicated that the essential genes for As-dependent BNF [i.e., nitrogen fixation, As(III) oxidation, and carbon fixation] were present in Serratia-associated metagenome-assembled genomes. Over 20 Serratia genomes obtained from NCBI also contained essential genes for both As(III) oxidation and BNF (i.e., aioA and nif H), suggesting that Asdependent BNF may be a widespread metabolic trait in Serratia spp.

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“…In this regard, it has been observed that members of the genus Acidocella are commonly found in acid tailings and can reduce different metals (Sağlam et al, 2016). Similarly, it has been observed that Burkholderia and Serratia have the ability to resist high concentrations of heavy metals, such as cadmium for Burkholderia and cadmium, cobalt, and zinc for Serratia (Zelaya-Molina et al, 2016;Wang et al, 2019).…”

Section: Microbiome Abundance and Diversitymentioning

confidence: 94%

Co-occurrence Interaction Networks of Extremophile Species Living in a Copper Mining Tailing

et al. 2022

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Copper mining tailings are characterized by high concentrations of heavy metals and an acidic pH, conditions that require an extreme adaptation for any organism. Currently, several bacterial species have been isolated and characterized from mining environments; however, very little is known about the structure of microbial communities and how their members interact with each other under the extreme conditions where they live. This work generates a co-occurrence network, representing the bacterial soil community from the Cauquenes copper tailing, which is the largest copper waste deposit worldwide. A representative sampling of six zones from the Cauquenes tailing was carried out to determine pH, heavy metal concentration, total DNA extraction, and subsequent assignment of Operational Taxonomic Units (OTUs). According to the elemental concentrations and pH, the six zones could be grouped into two sectors: (1) the “new tailing,” characterized by neutral pH and low concentration of elements, and (2) the “old tailing,” having extremely low pH (~3.5) and a high concentration of heavy metals (mainly copper). Even though the abundance and diversity of species were low in both sectors, the Pseudomonadaceae and Flavobacteriaceae families were over-represented. Additionally, the OTU identifications allowed us to identify a series of bacterial species with diverse biotechnological potentials, such as copper bioleaching and drought stress alleviation in plants. Using the OTU information as a template, we generated co-occurrence networks for the old and new tailings. The resulting models revealed a rearrangement between the interactions of members living in the old and new tailings, and highlighted conserved bacterial drivers as key nodes, with positive interactions in the network of the old tailings, compared to the new tailings. These results provide insights into the structure of the soil bacterial communities growing under extreme environmental conditions in mines.

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Ammonia-Oligotrophic and Diazotrophic Heavy Metal-Resistant Serratia liquefaciens Strains from Pioneer Plants and Mine Tailings

Cited by 13 publications

References 136 publications

Halotolerant Endophytic Bacterium Serratia rubidaea ED1 Enhances Phosphate Solubilization and Promotes Seed Germination

Halotolerant Endophytic Bacterium Serratia rubidaea ED1 Enhances Phosphate Solubilization and Promotes Seed Germination

Serratia spp. Are Responsible for Nitrogen Fixation Fueled by As(III) Oxidation, a Novel Biogeochemical Process Identified in Mine Tailings

Co-occurrence Interaction Networks of Extremophile Species Living in a Copper Mining Tailing

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