Ammonia nitrogen exposure caused structural damages to gill mitochondria of clam Ruditapes philippinarum

Cong, Ming; Li, Yuanmei; Xu, Hongchao; Lv, Jiasen; Wu, Huifeng; Zhao, Ye

doi:10.1016/j.ecoenv.2021.112528

Cited by 21 publications

(6 citation statements)

References 31 publications

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“…Pulmonary fibrosis remains the primary cause of mortality due to intricate etiology and limited treatment options (Kinoshita & Goto, 2019). Ammonia, a corrosive gas widely used in animal houses, is known to actively induce various pathological diseases in humans and animals (Cong et al., 2021; Wyer et al., 2022; Zhou et al., 2022). Recent studies have documented that chronic ammonia inhalation can result in pulmonary fibrosis (Brautbar et al., 2003; Witt et al., 2017).…”

Section: Discussionmentioning

confidence: 99%

Platycodin D ameliorates ammonia‐induced pulmonary fibrosis by repressing TGF‐β1‐mediated extracellular matrix remodeling

Lian,

Ge,

Pang

2024

Chem Biol Drug Des

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Ammonia can induce pulmonary fibrosis in humans and animals. Platycodin D (PLD) possesses various bioactive activities including anti‐fibrotic properties. In this study, we aimed to explore the activity and mechanism of PLD in pulmonary fibrosis induced by ammonia. The mouse model of ammonia‐induced lung fibrosis was established, and the role of PLD was assessed by H&E and Masson's trichrome staining. The differentially expressed genes (DEGs) were identified by RNA‐seq and subjected to GO and KEGG pathway analyses. BEAS‐2B cells were treated with NH4Cl alone or along with PLD. Results showed that PLD attenuated ammonia‐induced pulmonary inflammation and fibrosis in vivo. The extracellular matrix (ECM)‐receptor interaction pathway was predicted as a prominent pathway underlying the anti‐fibrotic function of PLD. In ammonia‐induced mouse models and NH4Cl‐treated BEAS‐2B cells, PLD could repress the activation of the TGF‐β1 pathway. By incubating lung fibroblast HFL1 cells with the conditioned medium of BEAS‐2B cells treated with NH4Cl alone or along with PLD, PLD was confirmed to attenuate NH4Cl‐induced ECM deposition in HFL1 cells. Our findings demonstrate that PLD exerts a protective function in ammonia‐induced pulmonary fibrosis by repressing TGF‐β1‐mediated ECM remodeling, suggesting the potential therapeutic value of PLD in this disease.

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Section: Discussionmentioning

confidence: 99%

Platycodin D ameliorates ammonia‐induced pulmonary fibrosis by repressing TGF‐β1‐mediated extracellular matrix remodeling

Lian,

Ge,

Pang

2024

Chem Biol Drug Des

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“…Abiotic stresses such as acute salt stress and ammonia nitrogen stress cause damage to bivalves. The decrease of salinity caused by rainstorms and the increase of ammonia nitrogen caused by pollutants could lead to large-scale shell sh death [31,32]. The expanded P-selectins in S. constricta exerted functions in adapting to the damage caused by environmental factors and provided a stronger injury repair ability by participating in the in ammatory response.…”

Section: Discussionmentioning

confidence: 99%

Massive expansion of P-selectin genes in two Venerida species, Sinonovacula constricta and Mercenaria mercenaria: evidence from comparative genomics of Bivalvia

Dong

et al. 2022

Preprint

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Background: P-selectin is an adhesion molecule participating in the inflammatory response essential for wound repair. However, studies regarding P-selectin in Bivalvia are rare. This study identified 90 P-selectin genes among nine bivalve genomes and classified them into 4 subfamilies according to phylogenetic analysis. Results: Notable P-selectin gene expansion was observed in two Venerida species, Sinonovacula constricta and Mercenaria mercenaria. The synteny analysis revealed that P-selectin gene expansion was mostly caused by tandem duplication. In addition, the expression profiles of P-selectin genes in S. constricta showed that many P-selectins were specifically highly expressed in the gills, and the P-selectin expression patterns changed dramatically under low salt stress and ammonia nitrogen stress. Conclusions: The massive expansion of P-selectins may facilitate the tolerance to environmental stresses. This study sheds light on the characterizations and expression profiles of P-selectin genes in Bivalvia and provides an integrated framework for further investigation of the role of P-selectins in the environmental tolerance of bivalves.

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“…Abiotic stresses such as acute salt stress and ammonia nitrogen stress cause damage to bivalves. The decrease of salinity caused by rainstorms and the increase of ammonia nitrogen caused by pollutants could lead to large-scale shellfish death [ 31 , 32 ]. The expanded P-selectins in S. constricta could exert functions in adapting to the damage caused by environmental factors and provide a stronger injury repair ability by participating in the inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

Massive expansion of P-selectin genes in two Venerida species, Sinonovacula constricta and Mercenaria mercenaria: evidence from comparative genomics of Bivalvia

Dong

et al. 2022

BMC Genomics

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Background P-selectin is a molecule participating in the inflammatory response through mediating cellular adhesion and essential for wound repair. However, studies regarding P-selectin in Bivalvia are rare. This study identified 90 P-selectin genes among nine bivalve genomes and classified them into 4 subfamilies according to phylogenetic analysis. Results Notable P-selectin gene expansion was observed in two Venerida species, Sinonovacula constricta and Mercenaria mercenaria. The synteny analysis revealed that P-selectin gene expansion was mostly caused by tandem duplication. In addition, the expression profiles of P-selectin genes in S. constricta showed that many P-selectins were specifically highly expressed in the gills, and the P-selectin expression patterns changed dramatically under low salt stress and ammonia nitrogen stress. Conclusions The massive expansion of P-selectins may facilitate the tolerance to environmental stresses. This study sheds light on the characterizations and expression profiles of P-selectin genes in Bivalvia and provides an integrated framework for further investigation of the role of P-selectins in the environmental tolerance of bivalves.

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Ammonia nitrogen exposure caused structural damages to gill mitochondria of clam Ruditapes philippinarum

Cited by 21 publications

References 31 publications

Platycodin D ameliorates ammonia‐induced pulmonary fibrosis by repressing TGF‐β1‐mediated extracellular matrix remodeling

Platycodin D ameliorates ammonia‐induced pulmonary fibrosis by repressing TGF‐β1‐mediated extracellular matrix remodeling

Massive expansion of P-selectin genes in two Venerida species, Sinonovacula constricta and Mercenaria mercenaria: evidence from comparative genomics of Bivalvia

Massive expansion of P-selectin genes in two Venerida species, Sinonovacula constricta and Mercenaria mercenaria: evidence from comparative genomics of Bivalvia

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