AML poor prognosis factor, TPD52, is associated with the maintenance of haematopoietic stem cells through regulation of cell proliferation

Abstract

Acute myeloid leukaemia (AML) is a blood cancer where undifferentiated myeloid cells are increased in the bone marrow and peripheral blood. As AML is dangerous and shows poor prognosis, many researchers categorised the relevant cytogenetic factors according to risk and prognosis. However, the specific reasons for poor cytogenetic factors remain unknown. We analysed a large data set from AML patients and found that TPD52 expression is elevated in patient groups with poor cytogenetic factors. As the amino acid sequence of TPD52 is evolutionally conserved in vertebrates, zebrafish embryos were used to investigate the function of TPD52. Since myeloid‐biased haematopoietic stem cells (HSCs) are relevant to AML, the function of TPD52 in the development of HSCs was investigated. We determined that the zebrafish paralog, tpd52, is important for the maintenance of HSCs through regulation of cell proliferation. As tpd52 is linked to cell proliferation in zebrafish embryos, the proliferation‐related gene, CD59, was correlated to TPD52 in every AML cohort with a high correlation coefficient. We suggest that TPD52 can be a novel therapeutic target for AML patients with poor cytogenetic factors. Additionally, more studies between TPD52 and CD59 will further increase the value of TPD52 as a novel target.

SOCS3 is Related to Cell Proliferation in Neuronal Tissue: An Integrated Analysis of Bioinformatics and Experiments

“…Furthermore, SOCS3 knockdown is related to cell proliferation in GBM cell lines. In previous studies, we showed that several cancer-related genes are involved in vertebrate development ( Oh et al, 2020 ; Kang et al, 2021 ). In addition to our previous studies, many studies have suggested that signaling pathways in developmental stages and oncology are highly similar.…”

“…In this study, we showed poor prognosis in both LGG and GBM when SOCS3 expression was high. Many researchers have identified novel functions of cancer-related genes during development, and we have previously shown that several cancer-related genes are involved in vertebrate development ( Oh et al, 2020 ; Kang et al, 2021 ). In the present study, we found that SOCS3, a poor prognostic marker of GBM, is important for brain development in vertebrates.…”

“…WISH was performed in 2 days post-fertilization (dpf) embryos. The overall process for WISH was conducted according to previous studies ( Oh et al, 2020 ; Kang et al, 2020 ).…”

Neuropeptide Y: a potential theranostic biomarker for diabetic peripheral neuropathy in patients with type-2 diabetes

“…Total RNA was isolated, and cDNA was synthesized as previously described. 16 A quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed using PowerUP SYBR Green Master Mix (Thermo Fisher Scientific, Waltham, MA, USA). The expression level of the target gene was normalized to that of β-actin as an endogenous control.…”

“…Zebrafish embryos were cultured using E3 solution in incubators at 28°C. 16 In vitro transcription for mRNA injection For overexpression experiments, full length was cloned into the pcs2p + vector. mRNA was synthesized using an mMESSAGE mMACHINE SP6 (Invitrogen, Carlsbad, CA, USA).…”