Aminopropyltransferases Involved in Polyamine Biosynthesis Localize Preferentially in the Nucleus of Plant Cells

Belda-Palazón, Borja; Ruiz, Leticia; Martí, Esmeralda; Tárraga, Susana; Tiburcio, Antonio F.; Culiáñez, Francisco; Farràs, Rosa; Carrasco, Pedro; Ferrando, Alejandro

doi:10.1371/journal.pone.0046907

Cited by 117 publications

(99 citation statements)

References 34 publications

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“…The BiFC assay was carried out basically as described by Belda-Palazón et al (2012). The full-length ORFs of ATS.1/ATS.2 and GAI/RGA were cloned in pCR8 and transferred by Gateway into the YFN43 and the YFC43 plasmid, respectively.…”

Section: Bifc Assaymentioning

confidence: 99%

Gibberellins Regulate Ovule Integument Development by Interfering with the Transcription Factor ATS

et al. 2016

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Gibberellins (GAs) are plant hormones that regulate most plant life cycle aspects, including flowering and fruit development. Here, we demonstrate the implication of GAs in ovule development. DELLA proteins, negative GA response regulators, act as positive factors for ovule integument development in a mechanism that involves transcription factor ABERRANT TESTA SHAPE (ATS). The seeds of the della global mutant, a complete loss-of-function of DELLA, and the ats-1 mutant are remarkably similar, with a round shape, a disorganized testa, and viviparism. These defects are the result of an alteration in integuments that fail to fully develop and are shorter than in wild-type plants. ats-1 also shows some GA-related phenotypes, for example, higher germination rates and early flowering. In fact, ats-1 has elevated GA levels due to the activation of GA biosynthesis genes, which indicates that ATS inhibits GA biosynthesis. Moreover, DELLAs and ATS proteins interact, which suggests the formation of a transcriptional complex that regulates the expression of genes involved in integument growth. Therefore, the repression of GA biosynthesis by ATS would result in the stabilization of DELLAs to ensure correct ATS-DELLA complex formation. The requirement of both activities to coordinate proper ovule development strongly argues that the ATS-DELLA complex acts as a key molecular factor. This work provides the first evidence for a role of GAs in ovule and seed development.

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Section: Bifc Assaymentioning

confidence: 99%

Gibberellins Regulate Ovule Integument Development by Interfering with the Transcription Factor ATS

et al. 2016

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“…The cDNA clones in the pDONR207 vector were recombined by LR Gateway reaction into the final pDestTH1 (bacterial MBP fusion expression vector) for MYB11 and ZML2 antibody testing, in pYFPN43 and pYFPC43 (Belda-Palazón et al, 2012) for BiFC, and in pGBKT7 and pGADT7 Gateway vectors for yeast two-hybrid mating assays.…”

Section: Dna Constructsmentioning

confidence: 99%

A MYB/ZML Complex Regulates Wound-Induced Lignin Genes in Maize

et al. 2015

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Lignin is an essential polymer in vascular plants that plays key structural roles in vessels and fibers. Lignification is induced by external inputs such as wounding, but the molecular mechanisms that link this stress to lignification remain largely unknown. In this work, we provide evidence that three maize (Zea mays) lignin repressors, MYB11, MYB31, and MYB42, participate in wound-induced lignification by interacting with ZML2, a protein belonging to the TIFY family. We determined that the three R2R3-MYB factors and ZML2 bind in vivo to AC-rich and GAT(A/C) cis-elements, respectively, present in a set of lignin genes. In particular, we show that MYB11 and ZML2 bind simultaneously to the AC-rich and GAT(A/C) cis-elements present in the promoter of the caffeic acid O-methyl transferase (comt) gene. We show that, like the R2R3-MYB factors, ZML2 also acts as a transcriptional repressor. We found that upon wounding and methyl jasmonate treatments, MYB11 and ZML2 proteins are degraded and comt transcription is induced. Based on these results, we propose a molecular regulatory mechanism involving a MYB/ZML complex in which wound-induced lignification can be achieved by the derepression of a set of lignin genes.

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“…Constructs were done in pSPYNE-35S (Walter et al, 2004) as well as gateway vector pYFP C 43 (a derivative of pMDC43 where GFP is replaced by YFP C ; Belda-Palazón et al, 2012). The coding sequence of At2g38310 (PYL4) was cloned into the pENTR223.1-Sfi entry vector, kindly provided by the Arabidopsis Biological Resource Center (clone G12806).…”

Section: Bifc Assay In Nicotiana Benthamianamentioning

confidence: 99%

The PYL4 A194T Mutant Uncovers a Key Role of PYR1-LIKE4/PROTEIN PHOSPHATASE 2CA Interaction for Abscisic Acid Signaling and Plant Drought Resistance

et al. 2013

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Because abscisic acid (ABA) is recognized as the critical hormonal regulator of plant stress physiology, elucidating its signaling pathway has raised promise for application in agriculture, for instance through genetic engineering of ABA receptors. PYRABACTIN RESISTANCE1/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS ABA receptors interact with high affinity and inhibit clade A phosphatases type-2C (PP2Cs) in an ABA-dependent manner. We generated an allele library composed of 10,000 mutant clones of Arabidopsis (Arabidopsis thaliana) PYL4 and selected mutations that promoted ABA-independent interaction with PP2CA/ABA-HYPERSENSITIVE3. In vitro protein-protein interaction assays and size exclusion chromatography confirmed that PYL4A194T was able to form stable complexes with PP2CA in the absence of ABA, in contrast to PYL4. This interaction did not lead to significant inhibition of PP2CA in the absence of ABA; however, it improved ABA-dependent inhibition of PP2CA. As a result, 35S: PYL4 A194T plants showed enhanced sensitivity to ABA-mediated inhibition of germination and seedling establishment compared with 35S:PYL4 plants. Additionally, at basal endogenous ABA levels, whole-rosette gas exchange measurements revealed reduced stomatal conductance and enhanced water use efficiency compared with nontransformed or 35S:PYL4 plants and partial up-regulation of two ABA-responsive genes. Finally, 35S:PYL4 A194T plants showed enhanced drought and dehydration resistance compared with nontransformed or 35S:PYL4 plants. Thus, we describe a novel approach to enhance plant drought resistance through allele library generation and engineering of a PYL4 mutation that enhances interaction with PP2CA.

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Aminopropyltransferases Involved in Polyamine Biosynthesis Localize Preferentially in the Nucleus of Plant Cells

Cited by 117 publications

References 34 publications

Gibberellins Regulate Ovule Integument Development by Interfering with the Transcription Factor ATS

Gibberellins Regulate Ovule Integument Development by Interfering with the Transcription Factor ATS

A MYB/ZML Complex Regulates Wound-Induced Lignin Genes in Maize

The PYL4 A194T Mutant Uncovers a Key Role of PYR1-LIKE4/PROTEIN PHOSPHATASE 2CA Interaction for Abscisic Acid Signaling and Plant Drought Resistance

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