Aminoguanidine treatment increased NOX2 response in diabetic rats: Improved phagocytosis and killing of Candida albicans by neutrophils

Ferreira, Cláudia de Souza; Pennacchi, Paula Comune; Araújo, Tomaz Henrique; Taniwaki, Noemi Nosomi; Paula, Fernanda Borges de Araújo; Duarte, Stella Maris da Silveira; Rodrigues, Maria Rita

doi:10.1016/j.ejphar.2015.12.044

Cited by 10 publications

(23 citation statements)

References 55 publications

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“…There are no studies investigating the downstream effects of hyperglycemia that are involved in the well-known complications of DM (polyol, AGE/RAGE, PKC, and hexosamine pathways). Interestingly, one study showed that inhibition of AGE formation in diabetic rats improved the microbicidal activity of PMNs against C. albicans (295). However, it should be noted that this study concerned peritoneal PMNs and Candida infection, but AGEs might be involved in oral candidal lesions as well.…”

Section: Potential Oral Complications Of Diabetes Mellitusmentioning

confidence: 99%

Evaluating All Potential Oral Complications of Diabetes Mellitus

et al. 2019

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Diabetes mellitus (DM) is associated with several microvascular and macrovascular complications, such as retinopathy, nephropathy, neuropathy, and cardiovascular diseases. The pathogenesis of these complications is complex, and involves metabolic and hemodynamic disturbances, including hyperglycemia, insulin resistance, dyslipidemia, hypertension, and immune dysfunction. These disturbances initiate several damaging processes, such as increased reactive oxygen species (ROS) production, inflammation, and ischemia. These processes mainly exert their damaging effect on endothelial and nerve cells, hence the susceptibility of densely vascularized and innervated sites, such as the eyes, kidneys, and nerves. Since the oral cavity is also highly vascularized and innervated, oral complications can be expected as well. The relationship between DM and oral diseases has received considerable attention in the past few decades. However, most studies only focus on periodontitis, and still approach DM from the limited perspective of elevated blood glucose levels only. In this review, we will assess other potential oral complications as well, including: dental caries, dry mouth, oral mucosal lesions, oral cancer, taste disturbances, temporomandibular disorders, burning mouth syndrome, apical periodontitis, and peri-implant diseases. Each oral complication will be briefly introduced, followed by an assessment of the literature studying epidemiological associations with DM. We will also elaborate on pathogenic mechanisms that might explain associations between DM and oral complications. To do so, we aim to expand our perspective of DM by not only considering elevated blood glucose levels, but also including literature about the other important pathogenic mechanisms, such as insulin resistance, dyslipidemia, hypertension, and immune dysfunction.

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Section: Potential Oral Complications Of Diabetes Mellitusmentioning

confidence: 99%

Evaluating All Potential Oral Complications of Diabetes Mellitus

et al. 2019

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“…Adherence to the vascular endothelium, neutrophil chemotaxis, phagocytosis and opsonization, intracellular bactericidal activity, and cell-mediated immunity are all decreased in DM patients with hyperglycemia [344,345]. Regarding this matter, Souza et al [346] treated diabetic rats with aminoguanidine (AMG, an inhibitor of protein glycation) and evaluated neutrophil reactive oxygen species (ROS) generation and C. albicans killing ability in order to evaluate the effects of hyperglycemia and the glycation of proteins on the NOX2 (phagocyte NADPH oxidase) activity of neutrophils and its implications for cellular physiology. The authors indicated that AMG increased the NOX2 response and microbicidal activity by neutrophils of the diabetic status.…”

Section: Candidiasis and Dmmentioning

confidence: 99%

“…The authors indicated that AMG increased the NOX2 response and microbicidal activity by neutrophils of the diabetic status. AMG seems to be a promising therapeutic answer for these patients [346].…”

Section: Candidiasis and Dmmentioning

confidence: 99%

Candida sp. Infections in Patients with Diabetes Mellitus

Rodrigues

Henriques

2019

JCM

201

161

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Candidiasis has increased substantially worldwide over recent decades and is a significant cause of morbidity and mortality, especially among critically ill patients. Diabetes mellitus (DM) is a metabolic disorder that predisposes individuals to fungal infections, including those related to Candida sp., due to a immunosuppressive effect on the patient. This review aims to discuss the latest studies regarding the occurrence of candidiasis on DM patients and the pathophysiology and etiology associated with these co-morbidities. A comprehensive review of the literature was undertaken. PubMed, Scopus, Elsevier’s ScienceDirect, and Springer’s SpringerLink databases were searched using well-defined search terms. Predefined inclusion and exclusion criteria were applied to classify relevant manuscripts. Results of the review show that DM patients have an increased susceptibility to Candida sp. infections which aggravates in the cases of uncontrolled hyperglycemia. The conclusion is that, for these patients, the hospitalization periods have increased and are commonly associated with the prolonged use of indwelling medical devices, which also increase the costs associated with disease management.

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“…It is well known that the phagocytic capacity of neutrophils is not a “fixed” cellular characteristic. Phagocytosis can be influenced by both external, e.g., temperature, medication, injuries ( 22 – 24 ), and internal factors, e.g., age, sepsis, auto-immune disease ( 25 – 27 ). Also, ex vivo manipulation can alter the function of a single sample of neutrophils with regard to phagocytosis ( 14 , 28 ).…”

Section: Discussionmentioning

confidence: 99%

Neutrophil Functional Heterogeneity: Identification of Competitive Phagocytosis

et al. 2017

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IntroductionPhagocytosis by neutrophils is a key process in the innate immune response against invading microorganisms. Despite reported heterogeneity in other neutrophils functions, little is known regarding differences in phagocytosis by individual cells. Therefore, we tested the hypothesis that heterogeneity is present in the neutrophil compartment in its potency to phagocytize bacteria.MethodsPhagocytosis assays were performed in suspension with isolated neutrophils and Staphylococcus aureus expressing different fluorescent proteins at MOIs between 1 and 10. Repetitive addition of bacteria with different fluorescent proteins and MOIs was used to compare the phagocytic capacity of S. aureus-green fluorescent protein (GFP)-positive and negative neutrophils and exclude randomness.ResultsThe percentage and mean fluorescence intensity (MFI) of S. aureus-GFP-positive neutrophils increased with higher MOIs. The increase in MFI was due to phagocytosis of multiple bacteria per neutrophil as was confirmed by confocal imaging. Sequential phagocytosis of GFP- and mCherry-expressing S. aureus showed a non-random process, as S. aureus-GFP-positive neutrophils preferentially phagocytized S. aureus-mCherry.ConclusionAll neutrophils were able to phagocytize S. aureus, but some were much more potent than others. Therefore, at physiologically relevant MOIs these potent phagocytizing neutrophils will outcompete the uptake of bacteria by less competent cells in a process we propose to name “competitive phagocytosis.”

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Aminoguanidine treatment increased NOX2 response in diabetic rats: Improved phagocytosis and killing of Candida albicans by neutrophils

Cited by 10 publications

References 55 publications

Evaluating All Potential Oral Complications of Diabetes Mellitus

Evaluating All Potential Oral Complications of Diabetes Mellitus

Candida sp. Infections in Patients with Diabetes Mellitus

Neutrophil Functional Heterogeneity: Identification of Competitive Phagocytosis

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