Aminoguanidine prevented impairment of blood antioxidant system in insulin-dependent diabetic rats

Stoppa, Graziela R.; Cesquini, Maristela; Roman, Erika; Ogo, Satie Hatsushika; Torsoni, Márcio Alberto

doi:10.1016/j.lfs.2005.07.031

Cited by 32 publications

(25 citation statements)

References 41 publications

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“…Besides, AG prevents impairment of blood antioxidant systems in insulin dependent diabetic rats. 42 A recent study shows that AG improves antioxidant status and attenuates lipid peroxidation in the brain of rats exposed to chronic stress. 43 Another recent study demonstrates that aminoguanidine can reduce the oxidative damage to proteins and hence protein carbonyl content.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of aminoguanidine against oxidative stress and bladder injury in cyclophosphamide‐induced hemorrhagic cystitis in rat

Abraham

Rabi

Selvakumar

2008

Cell Biochemistry & Function

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Cyclophosphamide (CP) is an antineoplastic agent that is used for the treatment of many neoplastic diseases. Hemorrhagic cystitis (HC) is a major dose limiting side effect of CP. Recent studies show that aminogaunidine, an inhibitor of inducible nitric oxide synthase is a potent antioxidant and prevents changes caused by oxidative stress such as depletion of antioxidant activity and tissue injury. The purpose of the study is to investigate the effect of aminoguanidine on parameters of oxidative stress, antioxidant enzymes and bladder injury caused by CP. Adult male rats were randomly divided into four groups. Control rats were administered saline; the AG control group received 200 mg/kg body wt of aminoguanidine; The CP group received a single injection of CP at the dose of 150 mg/kg body wt intraperitoneally. The CP þAG group received aminoguanidine (200 mg/kg body wt) intraperitoneally 1 h before the administration of CP. The rats were sacrificed 16 h after CP/saline administration. The bladder was used for light microscopic studies and biochemical studies. The markers of oxidative damage including protein carbonyl content, protein thiol, malondialdehyde and conjugated dienes were assayed in the homogenates along with the activities of the antioxidant enzymes, superoxide dismutase, glutathione peroxidase, catalase, and glutathione reductase and glutathione S transferase. In the bladders of CP treated rats edema of lamina propria with epithelial and sub-epithelial hemorrhage was seen. All the parameters of oxidative stress that were studied were significantly elevated in the bladders of CP treated rats. The activities of the antioxidant enzymes were significantly lowered in the bladders of CP treated rats. Aminoguanidine pretreatment prevented CP-induced oxidative stress, decrease in the activities of anti-oxidant enzymes and reduced bladder damage. The results of the present study suggest the antioxidant role for aminoguanidine in CP-induced bladder damage.

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Section: Discussionmentioning

confidence: 99%

Protective effect of aminoguanidine against oxidative stress and bladder injury in cyclophosphamide‐induced hemorrhagic cystitis in rat

Abraham

Rabi

Selvakumar

2008

Cell Biochemistry & Function

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“…Diabetes is characterized by the occurrence of multiple serious vascular complications such as atherosclerosis and hypertension (3)(4)(5). Atherosclerotic process, pathologically manifested as arterial narrowing due to medial calcification and thickening, is the major feature of diabetic macroangiopathy (6). Proliferation of vascular smooth muscle cells (SMC) plays a central role in the development of atherosclerosis (7).…”

Section: Introductionmentioning

confidence: 99%

Ameliorative effects of aminoguanidine on rat aorta in Streptozotocin-induced diabetes and evaluation of α-SMA expression

Elbe¹,

Vardı²,

Orman³

et al. 2014

Anadolu Kardiyol Derg

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Objective: Diabetes mellitus is one of the chronic metabolic diseases which is characterized by microvascular and macrovascular complications. This study was designed to investigate the improving the effects of amnioguanidine on aortic damage in a streptozotocin (STZ) induced diabetic rat model. Methods: Thirty-two male Sprague-Dawley rats divided into four groups as follows: Control, Aminoguanidine, Diabetes, and Diabetes+Aminoguanidine. Experimental diabetes was induced by single dose STZ (45 mg/kg) intraperitoneally. After administration of STZ, the DM+AMG group began to receive AMG (1 g/L) was prepared by dissolving in tap water during 10 weeks. At the end of the study, blood glucose levels were determined and rats were sacrified by ketamine anesthesia. Following routine tissue process, aortas were embedded in paraffin. Histochemical (H-E and Orcein) and immunohistochemical α-smooth muscle actin (α-SMA) stains were applied and the sections examined by light microscope. Statistical analysis was carried out using the SPSS 13.0 statistical program. Results: The rats in diabetes group had significantly higher blood glucose levels than the rats of control. The main histological alterations were detected in tunica media such as extensive thickening (414.32±9.62 μm), irregular of elastic fibers and intensive α-SMA staining in diabetic rats. The thickness of tunica media was statistically increased in DM group, when compared with the control group (p<0.001). On the other hand, AMG prevented disorganization of elastic fibers and overexpression of α-SMA. The mean thickness of tunica media was decreased significantly in DM+AMG (319.16±6.53 μm) compared with the DM group (p<0.001).Conclusion: Our results demonstrate that AMG treatment may protect the impairment of aort structure at histological level.

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“…Moreover, there is increased carbonyl stress (Kume et al, 2005), polyol pathway (Oyama et al, 2006) and protein kinase C-dependent activation (Kume et al, 2005). In this sense, increased malondialdehyde concentration (MDA), both in patients (Martìn-Gallán et al, 2003;Tan et al, 2004;Valko et al, 2007) and diabetic animals (Stoppa et al, 2006), has been reported in plasma, erythrocytes, total blood and diverse tissues such as brain, liver, kidney and heart.…”

Section: Introductionmentioning

confidence: 99%

“…There is also evidence that hyperglycemia may reduce antioxidant defense mechanisms by altering antioxidant enzymes, impairing glutathione metabolism, and lowering concentrations of glutathione and vitamins C, E, A and D (Rahimi et al, 2005;Stoppa et al, 2006). Persistent hyperglycemia increases polyol pathway flux, as well as ROS generation rates and AGEs formation, leading to increased GSH oxidation (Oyama et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Relationship between oxidative stress and diabetic osteopenia in premenopausal rats

Duarte

Rodrigues

Rezende

et al. 2010

Braz. J. Pharm. Sci.

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The relationship between lipid peroxidation, antioxidant defense and diabetic osteopenia remains unclear. This study evaluated the relationship among lipid peroxidation index, antioxidant defense parameters and bone metabolism in a premenopausal diabetic model using measures including thiobarbituric acidreactive substances concentration (TBARS) and reduced glutathione (GSH) content in brain homogenates, histomorphometric analysis, biomechanical testing and bone mineral density (BMD). Female Wistar rats with regular estrous cycle were divided into two groups: Group 1: control rats (n = 15) and Group 2: diabetic rats (n = 15). Diabetes was induced by alloxan and confirmed by glycemia ≥250 mg/dL. The lipid peroxidation index, measured by TBARS concentration, showed a significant increase (p<0.05) in diabetic animals in comparison to control animals. However, the antioxidant parameter measured by GSH content, was significantly lower (p<0.05) in diabetic animals. Histomorphometric analysis showed a significant increase (p<0.05) in femoral trabecular separation together with a significant decrease (p<0.05) in trabecular thickness, and reduced trabecular bone volume in diabetic rats. Moreover, biomechanical testing and BMD values were significantly lower (p<0.05) in the diabetic group. Thus, our results demonstrated that increased lipid peroxidation and altered antioxidant defense could be related to the development of oxidative stress and diabetic osteopenia in premenopausal rats.Uniterms: Oxidative stress. Lipid peroxidation. Diabetes mellitus. Diabetic osteopenia/experimental study.A relação entre peroxidação lipídica, defesa antioxidante e osteopenia diabética permanece obscura. Este estudo avaliou a associação entre índice de peroxidação lipídica, parâmetro de defesa antioxidante e metabolismo ósseo em um modelo diabético pré-menopausa através de medidas como a concentração de substâncias reativas ao ácido tiobarbitúrico (SRAT) e conteúdo de glutationa reduzida (GSH) no homogenato cerebral, análises histomorfométricas, teste biomecânico e densidade mineral óssea (DMO). Ratos Wistar fêmeas com ciclo estral regular foram distribuídos em dois grupos: Grupo 1 -ratas controle (n = 15) e Grupo 2 -ratas diabéticas (n = 15). O diabetes foi induzido pela aloxana e confirmado pela glicemia ≥250 mg/dL. O índice de peroxidação lipídica, medido pela concentração de SRAT, demonstrou um aumento significativo (p<0.05) nos animais diabéticos, em relação aos animais controle. Entretanto, o parâmetro de defesa antioxidante, mensurado pelo conteúdo de GSH, foi reduzido significativamente (p<0.05) nos animais diabéticos. As análises histomorfométricas mostraram um aumento significativo (p<0.05) da separação trabecular do fêmur, associado à diminuição significativa da espessura trabecular (p<0.05) e volume ósseo trabecular reduzido nas ratas diabéticas. Além disso, o teste biomecânico, V.M.G. Duarte, A. de S. Rodrigues, L.A. de Rezende, A.Ma. de O. Ramos, R.M. de Souza, F.P.F. Neto, A. da C. Medeiros 540 medido pela força máxima, e val...

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Aminoguanidine prevented impairment of blood antioxidant system in insulin-dependent diabetic rats

Cited by 32 publications

References 41 publications

Protective effect of aminoguanidine against oxidative stress and bladder injury in cyclophosphamide‐induced hemorrhagic cystitis in rat

Protective effect of aminoguanidine against oxidative stress and bladder injury in cyclophosphamide‐induced hemorrhagic cystitis in rat

Ameliorative effects of aminoguanidine on rat aorta in Streptozotocin-induced diabetes and evaluation of α-SMA expression

Relationship between oxidative stress and diabetic osteopenia in premenopausal rats

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