Aminoaldehyde dehydrogenase activity during wound healing of mechanically injured pea seedlings

Modulation of polyamine catabolism in pea seedlings by calcium during salinity stress

“…Although pea AMADH does not oxidise betaine aldehyde at all, its N-terminal amino acid sequence surprisingly resembles those of various BADHs (Šebela et al 2000a). The participation of pea AMADH in adaptation to stress caused by mechanical damage, which involves polyamine catabolism, GABA generation and lignification, has already been demonstrated in pea plants (Petřivalský et al 2007).…”

“…GABA is involved in various physiological processes in plants including the regulation of cytosolic pH, carbon influx into the tricarboxylic acid cycle, protection against oxidative stress and cellular signalling (Kinnersley & Turano 2000;Bouché & Fromm 2004). Recently, a significant increase in the GABA level was demonstrated in pea seedlings following mechanical damage (Petřivalský et al 2007). The measured levels of DAO, AMADH, and POX activity in pea shoots and roots are in accordance with the previously published results (Angelini et al 1990;Petřivalský et al 2007).…”

“…Recently, a significant increase in the GABA level was demonstrated in pea seedlings following mechanical damage (Petřivalský et al 2007). The measured levels of DAO, AMADH, and POX activity in pea shoots and roots are in accordance with the previously published results (Angelini et al 1990;Petřivalský et al 2007). The lowest polyamine content and activities of the studied enzymes were detected in plants grown in 10mM NaCl or KCl.…”

Nutritional Evaluation of an Outstanding Apple Cultivar, 'Göbek' (<i>Malus</i><i> </i><i>communis</i> L.)

“…Free polyamine extraction and analysis were performed following the method described by Hwang et al [8] and Petřivalský et al [9], with minor modifications. Lyophilized peel and flesh parts of the apple fruit samples (150 mg) were extracted with 5% (v/v) trichloracetic acid (TCA) and derivatized with benzoyl chloride.…”

“…Lyophilized peel and flesh parts of the apple fruit samples (150 mg) were extracted with 5% (v/v) trichloracetic acid (TCA) and derivatized with benzoyl chloride. Different concentrations of polyamine standard solutions were prepared to produce calibration curves for putrescine, cadaverine, spermidine and spermine using the methodology described above [8,9]. Benzyolated derivatives of polyamines from the peel and flesh samples and standards were extracted from reaction mixtures with diethyl ether (3x15 mL), evaporated to dryness at 50°C under nitrogen gas, and dissolved in 120 µL of initial mobile phase.…”