Aminoacyl‐tRNA synthetase – a molecular multitasker

Gupta, Swadha; Jani, Jaykumar; Vijayasurya,; Mochi, Jigneshkumar; Tabasum, Saba; Sabarwal, Akash; Pappachan, Anju

doi:10.1096/fj.202202024rr

Cited by 7 publications

(4 citation statements)

References 212 publications

(545 reference statements)

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“…As we all know, amino acids must be activated to obtain extra energy before being incorporated into the peptide chain, and the activation reaction is proceeded under the catalysis of aminoacyl‐tRNA synthetase. [ 33 ] The activated amino acids and tRNA form aminoacyl‐tRNA ensuring the accurate translation of the genetic code and plays an important role in protein synthesis. [ 34 ] Aminoacyl‐tRNA is an important part of the cell wall that can give it strength and shape.…”

Section: Resultsmentioning

confidence: 99%

Compartmentalization into Outer and Inner Shells of Hollow Nanospheres for Antibiosis Based on Chemistry and Physical Damages

Sheng,

Ding,

Guo

2024

Adv Healthcare Materials

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There is a substantially ascending demand for nonantibiotic strategies to overcome the resistance of bacterial infections. Here, we report the discovery of a distinctive antibacterial structure. The novel structure of nanoparticle strategy is proposed for appreciable bacteria killing by the smart design of the delayed addition of crosslinkers into the reaction mixture. [2‐(methacryloyloxy)ethyl]trimethylammonium chloride solution (MTCl), a water‐soluble ionic liquid (IL), it has narrow‐size material distribution, good whiteness and high weather resistance. The quaternary ammonium salt has been utilized to efficiently permeate cell membrane through electrostatic interaction, accordingly, boasting a beneficiary of antibacterial properties. More importantly, it allows bacterium easily to attach the nanomaterials, especially the double shelled nanosphere. In the light of the introduction of 9‐amino(9‐deoxy)ep‐quinine (QNNH2) on its inner shell, it blocks the nucleic acid and glucose metabolism in bacteria, which is betterment of the antibacterial activity of double shelled structure nanoparticle compared to other structure of nanomaterials. This physical/chemical/biological triple antibacterial process eliminates the need for traditional antibiotics, and the fabrication strategies and material properties described here provide insights into the design of antibacterial nanomaterials based on chemical and physical effects.This article is protected by copyright. All rights reserved

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Section: Resultsmentioning

confidence: 99%

Compartmentalization into Outer and Inner Shells of Hollow Nanospheres for Antibiosis Based on Chemistry and Physical Damages

Sheng,

Ding,

Guo

2024

Adv Healthcare Materials

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“…Tryptophan tRNA synthetase (WARS), catalyzes the addition of the tryptophan amino acid to the cognate tRNA [102]. There are two WARS paralogues that function in the cytoplasm (WARS1) and mitochondria (WARS2), respectively.…”

Section: Towards Our Goal Of Establishing If Zikv Infection Of Cultur...mentioning

confidence: 99%

Zika virus infection in a cell culture model reflects the transcriptomic signatures in patients

Berglund,

Lennon,

Badu

et al. 2024

Preprint

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Zika virus (ZIKV), a re-emerging flavivirus, is associated with devasting developmental and neurological disease outcomes particularly in infants infected in utero. Towards understanding the molecular underpinnings of the unique ZIKV disease pathologies, numerous transcriptome-wide studies have been undertaken. Notably, these studies have overlooked the assimilation of RNA-seq analysis from ZIKV-infected patients with cell culture model systems. In this study we find that ZIKV-infection of human lung adenocarcinoma A549 cells, mirrored both the transcriptional and alternative splicing profiles from previously published RNA-seq data of peripheral blood mononuclear cells collected from pediatric patients during early acute, late acute, and convalescent phases of ZIKV infection. Our analyses show that ZIKV infection in cultured cells correlates with transcriptional changes in patients, while the overlap in alternative splicing profiles was not as extensive. Overall, our data indicate that cell culture model systems support dissection of select molecular changes detected in patients and establishes the groundwork for future studies elucidating the biological implications of alternative splicing during ZIKV infection.

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“…These newly added components broadened aaRS functionality by enabling better recognition of cognate tRNA molecules [10] and proofreading of mischarged tRNAs [11]. Beyond translation, these additional domains contributed to the non-canonical functions of aaRS in gene regulation, cell signaling, inflammation, angiogenesis, tumorigenesis, immune processes, and RNA viral infections [12][13][14][15][16][17][18].…”

Section: The Importance Of Aminoacyl Trna-synthetasesmentioning

confidence: 99%

Protein–Protein Interactions of Seryl-tRNA Synthetases with Emphasis on Human Counterparts and Their Connection to Health and Disease

Dulic,

Godinic-Mikulcic,

Kekez

et al. 2024

Life

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Seryl-tRNA synthetases (SerRSs), members of the aminoacyl-tRNA synthetase family, interact with diverse proteins, enabling SerRSs to enhance their role in the translation of the genetic message or to perform alternative functions in cellular processes beyond translation. Atypical archaeal SerRS interacts with arginyl-tRNA synthetase and proteins of the ribosomal P-stalk to optimize translation through tRNA channeling. The complex between yeast SerRS and peroxin Pex21p provides a connection between translation and peroxisome function. The partnership between Arabidopsis SerRS and BEN1 indicates a link between translation and brassinosteroid metabolism and may be relevant in plant stress response mechanisms. In Drosophila, the unusual heterodimeric mitochondrial SerRS coordinates mitochondrial translation and replication via interaction with LON protease. Evolutionarily conserved interactions of yeast and human SerRSs with m3C32 tRNA methyltransferases indicate coordination between tRNA modification and aminoacylation in the cytosol and mitochondria. Human cytosolic SerRS is a cellular hub protein connecting translation to vascular development, angiogenesis, lipogenesis, and telomere maintenance. When translocated to the nucleus, SerRS acts as a master negative regulator of VEGFA gene expression. SerRS alone or in complex with YY1 and SIRT2 competes with activating transcription factors NFκB1 and c-Myc, resulting in balanced VEGFA expression important for proper vascular development and angiogenesis. In hypoxia, SerRS phosphorylation diminishes its binding to the VEGFA promoter, while the lack of nutrients triggers SerRS glycosylation, reducing its nuclear localization. Additionally, SerRS binds telomeric DNA and cooperates with the shelterin protein POT1 to regulate telomere length and cellular senescence. As an antitumor and antiangiogenic factor, human cytosolic SerRS appears to be a promising drug target and therapeutic agent for treating cancer, cardiovascular diseases, and possibly obesity and aging.

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Aminoacyl‐tRNA synthetase – a molecular multitasker

Cited by 7 publications

References 212 publications

Compartmentalization into Outer and Inner Shells of Hollow Nanospheres for Antibiosis Based on Chemistry and Physical Damages

Compartmentalization into Outer and Inner Shells of Hollow Nanospheres for Antibiosis Based on Chemistry and Physical Damages

Zika virus infection in a cell culture model reflects the transcriptomic signatures in patients

Protein–Protein Interactions of Seryl-tRNA Synthetases with Emphasis on Human Counterparts and Their Connection to Health and Disease

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