Amino-functionalized monolayers covalently grafted to silica-based substrates as a robust primer anchorage in aqueous media

Giraud, Lucie; Nadarajah, Rakini; Matar, Yasmine; Bazin, Gwénaëlle; Sun, Jing; Zhu, Jiale; Giasson, Suzanne

doi:10.1016/j.apsusc.2016.02.141

Cited by 20 publications

(36 citation statements)

References 48 publications

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“…Hydrophobic monolayers have shown to remain stable in water over several days(Fig. S2)as it was previously reported for amino monolayers [50].…”

supporting

confidence: 78%

“…suggesting that the amino groups of the AUTES monolayers are protonated in Milli-Q water, as previously reported,[50] which should give rise to electrostatic interactions. However, such interactions were not detectable because of the important contribution of long-ranged attractive interactions (ca 300 nm) most probably arising from hydrophobic interactions between the apposing alkyl chains of the two AUTES monolayers.…”

supporting

confidence: 77%

“…The positively charged aminofunctionalized mica substrate displayed also a smooth surface with a water contact angle of 78°, a surface zeta potential of +40 mV and a thickness of 2.5 nm (Table 1). The nonnull contact angle is associated with a small portion of the alkyl chain of aminoalkylsilane molecules which can be exposed to the aqueous surrounding environment, as previously demonstrated [50]. Hydrophobic monolayers have shown to remain stable in water over several days(Fig.…”

supporting

confidence: 63%

“…4.a) corresponding to twice the thickness of an AUTES monolayer previously determined. [50] The force-distance profile between two aminofunctionalized mica surfaces across the ss-siRNA solution is purely repulsive but does not exhibit long-range exponential decaying forces (Fig. 4.a) suggesting the presence of steric forces associated with the adsorbed ss-siRNA.…”

Section: Hydrophobized-mica Surfacementioning

confidence: 96%

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Single stranded siRNA complexation through non-electrostatic interactions

et al. 2017

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As double stranded, single stranded siRNA (ss-siRNA) has demonstrated gene silencing activity but still requires efficient carriers to reach its cytoplasmic target. To better understand the fundamental aspect driving the complexation of ss-siRNA with nanocarriers, the interactions between surfaces of various compositions across a ss-siRNA solution were investigated using the Surface Forces Apparatus. The results show that ss-siRNA can adsorb onto hydrophilic (positively and negatively charged) as well as on hydrophobic substrates suggesting that the complexation can occur through hydrophobic interactions and hydrogen bonding in addition to electrostatic interactions. Moreover, the binding strength and the conformation of ss-siRNA depend on the nature of the interactions between the ss-siRNA and the surfaces. The binding of ss-siRNA with nanocarriers, such as micelles or liposomes through non-electrostatic interactions was also evidenced by a SYBR Gold cyanine dye. We evidenced the presence of interactions between the dye and oligonucleotides already complexed to non-cationic nanovectors biasing the quantification of the encapsulation. These results suggest that non-electrostatic interactions could be exploited to complement electrostatic interactions in the design of nanocarriers. In particular, the different highlighted interactions can be used to complex ss-siRNA with uncharged or anionic carriers which are related to lower toxicity compared to cationic carriers.

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“…Hydrophobic monolayers have shown to remain stable in water over several days(Fig. S2)as it was previously reported for amino monolayers [50].…”

supporting

confidence: 78%

supporting

confidence: 77%

supporting

confidence: 63%

Section: Hydrophobized-mica Surfacementioning

confidence: 96%

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Single stranded siRNA complexation through non-electrostatic interactions

et al. 2017

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“…The excess Si-OH groups condense among themselves to form a siloxane network (Si-O-Si), which resists to aggressive species. However, the so-formed Al-O-Si and Si-O-Si bonds were susceptible to hydrolysis at high pH and the hydrolytic stability of these bonds was close to the molecular structures, such as the length of the alkyl chains 13 and the type of terminal groups. [14][15][16] Herein, a bis-silane, bis-(g-triethoxysilylpropyl)-tetrasulde (KH-Si69, BT), was used.…”

Section: Introductionmentioning

confidence: 99%

Dynamic corrosion behavior of superhydrophobic surfaces

Zhu

et al. 2018

RSC Adv.

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Biochemistry strategies for label-free optical sensor biofunctionalization: advances towards real applicability

Soler

Lechuga

2021

Anal Bioanal Chem

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Label-free biosensors, and especially those based on optical transducers like plasmonic or silicon photonic systems, have positioned themselves as potential alternatives for rapid and highly sensitive clinical diagnostics, on-site environmental monitoring, and for quality control in foods or other industrial applications, among others. However, most of the biosensor technology has not yet been transferred and implemented in commercial products. Among the several causes behind that, a major challenge is the lack of standardized protocols for sensor biofunctionalization. In this review, we summarize the most common methodologies for sensor surface chemical modification and bioreceptor immobilization, discussing their advantages and limitations in terms of analytical sensitivity and selectivity, reproducibility, and versatility. Special focus is placed on the suggestions of innovative strategies towards antifouling and biomimetic functional coatings to boost the applicability and reliability of optical biosensors in clinics and biomedicine. Finally, a brief overview of research directions in the area of device integration, automation, and multiplexing will give a glimpse of the future perspectives for label-free optical biosensors.

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Amino-functionalized monolayers covalently grafted to silica-based substrates as a robust primer anchorage in aqueous media

Cited by 20 publications

References 48 publications

Single stranded siRNA complexation through non-electrostatic interactions

Single stranded siRNA complexation through non-electrostatic interactions

Dynamic corrosion behavior of superhydrophobic surfaces

Biochemistry strategies for label-free optical sensor biofunctionalization: advances towards real applicability

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