Amino Acids as Regulators of Cell Metabolism

Nesterov, Semen V.; Yaguzhinsky, L. S.; Podoprigora, G. I.; Nartsissov, Yaroslav R.

doi:10.1134/s000629792004001x

Cited by 16 publications

(11 citation statements)

References 102 publications

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“…Amino acids (AAs) are important as substrates for signaling molecules regulating metabolism or protein synthesis, as well as a source of energy [31]. Alterations in the circulating levels of AAs could be indicative of alterations in protein synthesis and degradation, as well as altered amino acid catabolism.…”

Section: Discussionmentioning

confidence: 99%

Serum Metabolic Profiling Identifies Key Differences between Patients with Single-Ventricle Heart Disease and Healthy Controls

Silva

Pietra

Baybayon-Grandgeorge

et al. 2022

IJTM

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There are growing numbers of infants and children living with single-ventricle congenital heart disease (SV). However, cardiac dysfunction and, ultimately, heart failure (HF) are common in the SV population and the ability to predict the progression to HF in SV patients has been limited, primarily due to an incomplete understanding of the disease pathogenesis. Here, we tested the hypothesis that non-invasive circulating metabolomic profiles can serve as novel biomarkers in the SV population. We performed systematic metabolomic and pathway analyses on a subset of pediatric SV non-failing (SVNF) and failing (SVHF) serum samples, compared with samples from biventricular non-failing (BVNF) controls. We determined that serum metabolite panels were sufficient to discriminate SVHF subjects from BVNF subjects, as well as SVHF subjects from SVNF subjects. Many of the identified significantly dysregulated metabolites were amino acids, energetic intermediates and nucleotides. Specifically, we identified pyruvate, palmitoylcarnitine, 2-oxoglutarate and GTP as promising circulating biomarkers that could be used for SV risk stratification, monitoring response to therapy and even as novel targets of therapeutic intervention in a population with few other options.

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Section: Discussionmentioning

confidence: 99%

Serum Metabolic Profiling Identifies Key Differences between Patients with Single-Ventricle Heart Disease and Healthy Controls

Silva

Pietra

Baybayon-Grandgeorge

et al. 2022

IJTM

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“…Optimal nutritional strategies after weaning are necessary to guarantee intestinal development and growth performance ( Mou et al., 2019 ). Some functional AA and their metabolites are key regulators of cell signaling and gene expression ( Wu, 2009 ; Nesterov et al., 2020 ). A thorough understanding of the process of AA metabolism in suckling and weanling period is therefore very important will help optimize the weaning transition of piglets.…”

Section: Discussionmentioning

confidence: 99%

Serum biochemical parameters and amino acids metabolism are altered in piglets by early-weaning and proline and putrescine supplementations

Wang

Xiao

et al. 2021

Animal Nutrition

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The study was to investigate the effect of early-weaning stress and proline (Pro) and putrescine (Put) supplementations on serum biochemical parameters and amino acids (AA) metabolism in suckling and post-weaning pigs. Blood and small intestinal mucosa were harvested from suckling piglets at 1, 7, 14, and 21 d of age and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age, as well as from piglets received oral administration of Pro and Put from 1 to 14 d old. In suckling piglets, the serum glucose, albumin and total cholesterol levels were increased ( P < 0.05) with increasing age, whereas the serum globulin, urea nitrogen (BUN), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) levels were lowered ( P < 0.05). The concentrations of most serum AA and the AA transporters related gene expressions were highest in 7-d-old piglets ( P < 0.05), whereas the phosphorylation status of the mammalian target of the rapamycin (mTOR) signaling pathway in the small intestine increased in piglets from 1 to 21 d old ( P < 0.05). Weaning at 14 d old increased ( P < 0.05) the BUN and triglycerides levels in serum, as well as jejunal solute carrier family 7 member 6 ( SLC7A6 ), ileal SLC36A1 and SLC1A1 mRNA abundances at d 1 or 3 post-weaning. Weaning also inhibited ( P < 0.05) the phosphorylation levels of mTOR and its downstream ribosomal protein S6 kinase 1 (S6K1) and 4E-binding protein-1 (4EBP1) in the small intestine of weanling pigs. Oral administration of Put and Pro decreased ( P < 0.05) serum ALP levels and increased ( P < 0.05) intestinal SLC36A1 and SLC1A1 mRNA abundances and mTOR pathway phosphorylation levels in post-weaning pigs. Pro but not Put treatment enhanced ( P < 0.05) serum Pro, arginine (Arg) and glutamine (Gln) concentrations of weaning-pigs. These findings indicated that early-weaning dramatically altered the biochemical blood metabolites, AA profile and intestinal mTOR pathway activity, and Pro and Put supplementations improved the AA metabolism and transportation as well as activated the intestinal mTOR pathway in weanling-pigs. Our study has an important implication for the broad application of Pro and Put in the weaning transition of piglets.

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“…Different cell states need specific metabolic programs to sustain the distinct bioenergetic demands underlying their specialized functions. Amino acids (AAs) are also essential as regulators of fluxes through major metabolic pathways [ 110 , 111 ]. The amino acid consumption profiles we recorded by analyzing the supernatants fell qualitatively in with measurements of Schop et al [ 56 ] and Higuera et al [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status

Muoio

Panella

Jossen

et al. 2021

JFB

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Human adipose stem cells (hASCs) are promising candidates for cell-based therapies, but they need to be efficiently expanded in vitro as they cannot be harvested in sufficient quantities. Recently, dynamic bioreactor systems operated with microcarriers achieved considerable high cell densities. Thus, they are a viable alternative to static planar cultivation systems to obtain high numbers of clinical-grade hASCs. Nevertheless, the production of considerable biomass in a short time must not be achieved to the detriment of the cells’ quality. To facilitate the scalable expansion of hASC, we have developed a new serum- and xeno-free medium (UrSuppe) and a biodegradable microcarrier (BR44). In this study, we investigated whether the culture of hASCs in defined serum-free conditions on microcarriers (3D) or on planar (2D) cell culture vessels may influence the expression of some marker genes linked with the immature degree or the differentiated status of the cells. Furthermore, we investigated whether the biomaterials, which form our biodegradable MCs, may affect cell behavior and differentiation. The results confirmed that the quality and the undifferentiated status of the hASCs are very well preserved when they grow on BR44 MCs in defined serum-free conditions. Indeed, the ASCs showed a gene expression profile more compatible with an undifferentiated status than the same cells grown under standard planar conditions.

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Amino Acids as Regulators of Cell Metabolism

Cited by 16 publications

References 102 publications

Serum Metabolic Profiling Identifies Key Differences between Patients with Single-Ventricle Heart Disease and Healthy Controls

Serum Metabolic Profiling Identifies Key Differences between Patients with Single-Ventricle Heart Disease and Healthy Controls

Serum biochemical parameters and amino acids metabolism are altered in piglets by early-weaning and proline and putrescine supplementations

Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status

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