Amino acids and amino acid sensing: implication for aging and diseases

Dato, Serena; Hoxha, Eneida; Crocco, Paolina; Iannone, Francesca; Passarino, Giuseppe; Rose, Giuseppina

doi:10.1007/s10522-018-9770-8

Cited by 33 publications

(22 citation statements)

References 107 publications

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“…This established method based on the UHPLC-QTRAP R / MS 2 could determine the nucleosides, nucleobases, and amino acids in safflower quickly and accurately. Safflower is rich in amino acids and high in content, especially Pro, Asp, Arg, Ser, and His, which could meet the needs of normal human growth, then maintain metabolic balance, improve cell viability, ensure the health of the body, and delay aging [35,36]. Furthermore, nucleosides and nucleobases could also play a beneficial role in health.…”

Section: Discussionmentioning

confidence: 99%

Analysis and evaluation of nucleosides, nucleobases, and amino acids in safflower from different regions based on ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Yue

Yan

et al. 2020

J of Separation Science

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Safflower has both medicinal and edible values but research on its nutrient composition is still lacking. This study was established for the quantitative determination of 28 nucleosides, nucleobases, and amino acids based on the ultra‐performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry. Analysis of 30 batches of safflower from different producing areas indicated that the contents of l‐proline, l‐asparagine, l(+)‐arginine, l‐serine, l‐histidine, uracil, guanosine, and uridine was high in safflower. Principle component analysis and cluster analysis found that samples from different regions could be distinguished well, and samples from the same area could be clustered into one class, different geographical environments may cause the differences of nucleosides, nucleobases, and amino acids in safflower. The analysis of principal component analysis, cluster analysis, and counter propagation artificial neural network show similar results. Then the content of nucleosides, nucleobases, and essential amino acids were compared, and found that the content in safflower from Gansu was higher than those from other regions, and there was a little difference between the samples from Xinjiang, Sichuan, and Yunnan. This research revealed the composition of nucleosides, nucleobases, and amino acids in safflower, and provided a theoretical basis for utilization of safflower.

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Section: Discussionmentioning

confidence: 99%

Analysis and evaluation of nucleosides, nucleobases, and amino acids in safflower from different regions based on ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Yue

Yan

et al. 2020

J of Separation Science

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“…Independently from its enzymatic activity, through protein–protein interaction, IPMK acts as a signaling hub in regulating nutrient and energetic pathways, including mTOR and AMPK [17,56]. The inhibition of mTORC1 pathway components extends lifespan and confers protection against an increasing list of age-related diseases, while on the contrary its over-induction leads to a higher risk of age-related diseases and decreased lifespan [57,58] and references therein]. A role in the control of cell survival and death has been also highlighted.…”

Section: Discussionmentioning

confidence: 99%

Inositol Polyphosphate Multikinase (IPMK), a Gene Coding for a Potential Moonlighting Protein, Contributes to Human Female Longevity

Rango

Crocco

Iannone

et al. 2019

Genes

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Biogerontological research highlighted a complex and dynamic connection between aging, health and longevity, partially determined by genetic factors. Multifunctional proteins with moonlighting features, by integrating different cellular activities in the space and time, may explain part of this complexity. Inositol Polyphosphate Multikinase (IPMK) is a potential moonlighting protein performing multiple unrelated functions. Initially identified as a key enzyme for inositol phosphates synthesis, small messengers regulating many aspects of cell physiology, IPMK is now implicated in a number of metabolic pathways affecting the aging process. IPMK regulates basic transcription, telomere homeostasis, nutrient-sensing, metabolism and oxidative stress. Here, we tested the hypothesis that the genetic variability of IPMK may affect human longevity. Single-SNP (single nuclear polymorphism), haplotype-based association tests as well as survival analysis pointed to the relevance of six out of fourteen genotyped SNPs for female longevity. In particular, haplotype analysis refined the association highlighting two SNPs, rs2790234 and rs6481383, as major contributing variants for longevity in women. Our work, the first to investigate the association between variants of IPMK and longevity, supports IPMK as a novel gender-specific genetic determinant of human longevity, playing a role in the complex network of genetic factors involved in human survival.

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“…Amino acid uptake mainly depends on amino acid transporters, and amino acid transporters are responsible for maintaining host AA metabolism balance or defending against pathogen infection by regulating AA levels [17,[33][34][35]. To determine whether the changes of Ser and Glu uptake are mediated through amino acid transporters in bEnd.3 cells at 1-h post-APEC XM infection, we measured the expression levels of Ser, Glu transporters.…”

Section: Apec Xm Infection Increases the Expression Of Snat2 And Eaat4 In Bend3 Cellsmentioning

confidence: 99%

The Amino Acid-mTORC1 Pathway Mediates APEC TW-XM-Induced Inflammation in bEnd.3 Cells

Zhang

Shu

Wang

et al. 2021

IJMS

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The blood–brain barrier (BBB) is key to establishing and maintaining homeostasis in the central nervous system (CNS); meningitis bacterial infection can disrupt the integrity of BBB by inducing an inflammatory response. The changes in the cerebral uptake of amino acids may contribute to inflammatory response during infection and were accompanied by high expression of amino acid transporters leading to increased amino acid uptake. However, it is unclear whether amino acid uptake is changed and how to affect inflammatory responses in mouse brain microvascular endothelial (bEnd.3) cells in response to Avian Pathogenic Escherichia coli TW-XM (APEC XM) infection. Here, we firstly found that APEC XM infection could induce serine (Ser) and glutamate (Glu) transport from extracellular into intracellular in bEnd.3 cells. Meanwhile, we also shown that the expression sodium-dependent neutral amino acid transporter 2 (SNAT2) for Ser and excitatory amino acid transporter 4 (EAAT4) for Glu was also significantly elevated during infection. Then, in amino acid deficiency or supplementation medium, we found that Ser or Glu transport were involving in increasing SNAT2 or EAAT4 expression, mTORC1 (mechanistic target of rapamycin complex 1) activation and inflammation, respectively. Of note, Ser or Glu transport were inhibited after SNAT2 silencing or EAAT4 silencing, resulting in inhibition of mTORC1 pathway activation, and inflammation compared with the APEC XM infection group. Moreover, pEGFP-SNAT2 overexpression and pEGFP-EAAT4 overexpression in bEnd.3 cells all could promote amino acid uptake, activation of the mTORC1 pathway and inflammation during infection. We further found mTORC1 silencing could inhibit inflammation, the expression of SNAT2 and EAAT4, and amino acid uptake. Taken together, our results demonstrated that APEC TW-XM infection can induce Ser or Glu uptake depending on amino acid transporters transportation, and then activate amino acid-mTORC1 pathway to induce inflammation in bEnd.3 cells.

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Amino acids and amino acid sensing: implication for aging and diseases

Cited by 33 publications

References 107 publications

Analysis and evaluation of nucleosides, nucleobases, and amino acids in safflower from different regions based on ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Analysis and evaluation of nucleosides, nucleobases, and amino acids in safflower from different regions based on ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Inositol Polyphosphate Multikinase (IPMK), a Gene Coding for a Potential Moonlighting Protein, Contributes to Human Female Longevity

The Amino Acid-mTORC1 Pathway Mediates APEC TW-XM-Induced Inflammation in bEnd.3 Cells

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