Amino acid uptake in arterio-venous serum of normal and cancerous colon tissues

Wang, Linbo; Shen, Jianguo; Zhang, Suzhan; Ding, Kefeng; Zheng, Shaojiang

doi:10.3748/wjg.v10.i9.1297

Cited by 13 publications

(16 citation statements)

References 22 publications

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“…These findings are in accordance with previous reports where an increase in amino acid turnover in breast cancer is reported [ 33 ]. In contrast, lower concentrations of various amino acids were observed in IDC serum, which is in agreement with previous report ( Supplementary Table 2 ) [ 34 ]. The diminished concentrations of the amino acids in IDC serum may be attributed to their higher uptake by the tumour tissue to fulfil the proliferative demand [ 34 ].…”

Section: Discussionsupporting

confidence: 93%

“…Increase in fatty acids in both serum and tissue is indicative of their de-novo synthesis in serum and uptake in tissue to support proliferation [ 49 ]. As discussed above, the lower levels of the amino acids in IDC serum could be due to their increased uptake by the tumour tissue leading to their accumulation in tissue and deprival in serum [ 34 ]. Other important metabolic changes shared by both tissue and serum were involved in antioxidant machinery, energy metabolism etc.…”

Section: Discussionmentioning

confidence: 99%

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Metabolomic alterations in invasive ductal carcinoma of breast: A comprehensive metabolomic study using tissue and serum samples

RoyChoudhury

Christie

et al. 2017

Oncotarget

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Invasive ductal carcinoma (IDC) is the most common type of breast cancer and the leading cause of breast cancer related mortality. In the present study, metabolomic profiles of 72 tissue samples and 146 serum samples were analysed using targeted liquid chromatography multiple reaction monitoring mass spectrometry (LC-MRM/MS) and untargeted gas chromatography mass spectrometry (GC-MS) approaches. Combination of univariate and multivariate statistical treatment identified significant alterations of 42 and 32 metabolites in tissue and serum samples of IDC, respectively when compared to control. Some of the metabolite changes from tissue were also reflected in serum, indicating a bi-directional interaction of metabolites in IDC. Additionally, 8 tissue metabolites and 9 serum metabolites showed progressive change from control to benign to IDC suggesting their possible role in malignant transformation. We have identified a panel of three metabolites viz. tryptophan, tyrosine, and creatine in tissue and serum, which could be useful in screening of IDC subjects from both control and benign. The metabolomic alterations in IDC showed perturbations in purine and pyrimidine metabolism, amino sugar metabolism, amino acid metabolism, fatty acid biosynthesis etc. Comprehensively, this study provides valuable insights into metabolic adaptations of IDC, which can help to identify diagnostic markers as well as potential therapeutic targets.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Metabolomic alterations in invasive ductal carcinoma of breast: A comprehensive metabolomic study using tissue and serum samples

RoyChoudhury

Christie

et al. 2017

Oncotarget

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“…This is consistent with the report that cancer cells may take up more essential amino acids [8]. The 875 cm -1 Raman peak due to hydroxyproline (a compound of collagen), and the 1445 cm -1 peak (associated with the CH 2 CH 3 bending modes of collagen), shows higher signals in cancer than in normal, confirming that the amount of collagen is increased in cancer tissue due to a desmoplastic response [6,8]. The Raman peak at 1320 cm -1 , associated with CH 3 CH 2 twisting mode of collagen/lipids, as well as the 1656 cm -1 peak, associated with the protein amide I band of collagen, being higher in cancer spectra further confirms the change of molecular structures like the deposition of greater amounts of collagen in cancer tissue compared to normal tissue [6].…”

Section: Discussionsupporting

confidence: 93%

“…For instance, the Raman peak of 1002 cm -1 which is associated with phenylanaline shows a higher signal in cancer tissue, indicating an increase in the essential amino acid phenelanaline relative to the total Raman-active components in the cancer tissue. This is consistent with the report that cancer cells may take up more essential amino acids [8]. The 875 cm -1 Raman peak due to hydroxyproline (a compound of collagen), and the 1445 cm -1 peak (associated with the CH 2 CH 3 bending modes of collagen), shows higher signals in cancer than in normal, confirming that the amount of collagen is increased in cancer tissue due to a desmoplastic response [6,8].…”

Section: Discussionsupporting

confidence: 90%

Near-infrared Raman spectroscopy for colonic cancer diagnosis

2005

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This study evaluated the diagnostic ability of near-infrared (NIR) Raman spectroscopy for identifying the malignant tumors from normal and benign tissues in the colon. In this work, a rapid NIR Raman system was utilized for tissue Raman studies. A total of 105 colonic specimens were used for Raman studies, including 41normals, 18 polyps (benign), and 46 malignant tumors (22 of moderately differentiated adenocarcinomas and 24 of poorly differentiated adenocarcinomas). The results showed that high-quality Raman spectra in the 800-1800 cm -1 range can be acquired from human colonic tissues in vitro, and Raman spectra differed significantly between normal and malignant tumor tissue. The diagnostic algorithm using the Raman intensity ratios of I 1085/1445 vs. I 1002/1445 can yield a diagnostic sensitivity of 100%and specificity of 96.6% for differentiation between between normal, benign and malignant colonic tissue. This work demonstrates that NIR Raman spectroscopy technique has a significant potential for the noninvasive diagnosis of colon cancer in vivo based on the evaluation of changes of molecular vibrations of biomolecules in tissue.

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“…For instance, Ala and Pro are biosynthesized from Glu via aminotransferase [ 53 ]. Tumor growth requires Glu, Gly, Asp and ammonia for purine and pyrimidine synthesis, which brings a selective demand on these metabolites [ 54 ]. Actually, our data showed that the levels of these amino acids in patients with different cancers were lower than those of healthy individuals.…”

Section: Discussionmentioning

confidence: 99%

Perioperative dynamics and significance of amino acid profiles in patients with cancer

Chen

et al. 2015

J Transl Med

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BackgroundMetabolome analysis including amino acid profile is under investigation as an approach in cancer screening. The present study aims to analyze plasma free amino acid (PFAA) profiles in cancer patients and investigate their potential as biomarkers of malignancy.MethodsPlasma samples from 56 gastric cancer patients, 28 breast cancer patients, 33 thyroid cancer patients, and 137 age-matched healthy controls were collected in the study. PFAA levels were measured and their perioperative alterations were analyzed. Biological effects of ten cancer-related amino acids were further validated in gastric and breast cancer cells.ResultsWe found that PFAA profiles of cancer patients differed significantly from those of healthy controls. Decreased concentrations of PFAAs were associated with lymph node metastases in gastric cancer. Levels of PFAAs such as aspartate and alanine increased after tumor resection. PFAA levels correlated with clinical tumor markers in gastric cancer patients and pathological immunohistochemistry markers in breast cancer patients. Specifically, alanine, arginine, aspartate and cysteine had proliferative effects on breast cancer cells. Proliferation of gastric cancer cells was promoted by cysteine, but inhibited by alanine and glutamic acid. Furthermore, alanine treatment decreased total and stable fraction of gastric cancer cells, and alanine and glutamic acid induced apoptosis of gastric cancer cells.ConclusionsPFAA patterns in cancer patients are altered perioperatively. Tumor-related amino acids identified by dynamic study of PFAA patterns may have the potential to be developed as novel biomarkers for diagnosis and prognosis of cancer patients.Electronic supplementary materialThe online version of this article (doi:10.1186/s12967-015-0408-1) contains supplementary material, which is available to authorized users.

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Amino acid uptake in arterio-venous serum of normal and cancerous colon tissues

Abstract: Abnormal synthetic metabolism of colon cancer may contribute to its higher amino acid uptake rate than that of normal colon.

Cited by 13 publications

References 22 publications

Metabolomic alterations in invasive ductal carcinoma of breast: A comprehensive metabolomic study using tissue and serum samples

Metabolomic alterations in invasive ductal carcinoma of breast: A comprehensive metabolomic study using tissue and serum samples

Near-infrared Raman spectroscopy for colonic cancer diagnosis

Perioperative dynamics and significance of amino acid profiles in patients with cancer

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