Amino acid transporter expression in the endometrium and conceptus membranes during early equine pregnancy

Gibson, Charlotte; Ruijter-Villani, Marta de; Rietveld, Jolanda; Stout, T.A.E.

doi:10.1071/rd17352

Cited by 15 publications

(21 citation statements)

References 37 publications

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“…We previously reported that negatively asynchronous ET delays conceptus development at the level of the whole conceptus and for selected genes known to exhibit parent-of-origin imprinting [40] or related to nutrient uptake [41,42]; the retardation in development presumably results from differences in histotroph composition due to the reduced duration of endometrial exposure to P4 prior to embryo introduction. The transcriptome comparisons performed between conceptus membranes at the different stages (D14-syn/asyn; D19-syn/asyn) indicated distinct profiles of DEGs, but gene ontology analysis revealed similar overrepresented categories to those reported previously between day 10, 12 and 14 compared to day 8 conceptuses by Klein and Troedsson [19].…”

Section: Discussionmentioning

confidence: 99%

“…For example, the embryonic disc (day 14) and embryo proper (day 19) are smaller and less well developed in conceptuses resulting after transfer of day 8 horse embryos into a 5-day negatively asynchronous recipient compared to a synchronous recipient [40]. In addition, the pregnancy stage-related upregulation in mRNA expression for a range of imprinted genes, glucose and amino acid transporters is delayed in conceptuses that develop in a negatively asynchronous uterus [40][41][42].…”

Section: Introductionmentioning

confidence: 99%

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Asynchronous Embryo Transfer Followed by Comparative Transcriptomic Analysis of Conceptus Membranes and Endometrium Identifies Processes Important to the Establishment of Equine Pregnancy

Gibson

Ruijter-Villani

Bauersachs

et al. 2020

IJMS

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Preimplantation horse conceptuses require nutrients and signals from histotroph, the composition of which is regulated by luteal progesterone and conceptus-secreted factors. To distinguish progesterone and conceptus effects we shortened the period of endometrial progesterone-priming by asynchronous embryo transfer. Day 8 embryos were transferred to synchronous (day 8) or asynchronous (day 3) recipients, and RNA sequencing was performed on endometrium and conceptuses recovered 6 and 11 days later (embryo days 14 and 19). Asynchrony resulted in many more differentially expressed genes (DEGs) in conceptus membranes (3473) than endometrium (715). Gene ontology analysis identified upregulation in biological processes related to organogenesis and preventing apoptosis in synchronous conceptuses on day 14, and in cell adhesion and migration on day 19. Asynchrony also resulted in large numbers of DEGs related to 'extracellular exosome'. In endometrium, genes involved in immunity, the inflammatory response, and apoptosis regulation were upregulated during synchronous pregnancy and, again, many genes related to extracellular exosome were differentially expressed. Interestingly, only 14 genes were differentially expressed in endometrium recovered 6 days after synchronous versus 11 days after asynchronous transfer (day 14 recipient in both). Among these, KNG1 and IGFBP3 were consistently upregulated in synchronous endometrium. Furthermore bradykinin, an active peptide cleaved from KNG1, stimulated prostaglandin release by cultured trophectoderm cells. The horse conceptus thus responds to a negatively asynchronous uterus by extensively adjusting its transcriptome, whereas the endometrial transcriptome is modified only subtly by a more advanced conceptus.Int. J. Mol. Sci. 2020, 21, 2562 2 of 25 to be essential for MRP, since restricting migration to a single uterine horn results in failure to avert luteolysis [7]. In the absence of pregnancy, luteolysis occurs between approximately days 13 and 16 as a result of pulses of prostaglandin F2α (PGF2α) released by the endometrium following the establishment of an oxytocin-PGF2α positive feedback loop [8,9]. To prevent luteal regression, the embryo's MRP signal must prevent the cyclical increase in oxytocin sensitivity that starts at approximately day 10 after ovulation [10,11]. Although the identity of the equine embryonic MRP signal(s) remains unknown [12], it is increasingly clear that MRP involves the transient suppression of PGF2α release by a combination of inhibiting prostaglandin-endoperoxidase-synthase 2 expression (PTGS2; the rate-limiting enzyme in PGF2α synthesis) and delaying the upregulation of the endometrial oxytocin receptors critical to the oxytocin-PGF2α loop needed to generate luteolytic pulses of PGF2α [8,10,13].Recent transcriptomic and proteomic analyses have improved our understanding of embryo-maternal communication during the conceptus migration and MRP phase. Studies have compared gene expression in endometrium between cycling and pregnant mares on days 8 an...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Asynchronous Embryo Transfer Followed by Comparative Transcriptomic Analysis of Conceptus Membranes and Endometrium Identifies Processes Important to the Establishment of Equine Pregnancy

Gibson

Ruijter-Villani

Bauersachs

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…We previously reported that negatively asynchronous ET delays conceptus development at the level of the whole conceptus and for selected genes known to exhibit parent-of-origin imprinting [38] or related to nutrient uptake [39,40]; the retardation in development presumably results from differences in histotroph composition due to the reduced duration of endometrial exposure to P4 prior to embryo introduction. The transcriptome comparisons performed between conceptus membranes at the different stages (D14-syn/asyn; D19-syn/asyn) indicated distinct profiles of DEGs, but gene ontology analysis revealed similar overrepresented categories to those reported previously between day 10, 12 and 14 compared to day 8 conceptuses by Klein and Troedsson [19].…”

Section: Discussionmentioning

confidence: 99%

“…All animal procedures were approved by Utrecht University's Animal Experimentation Committee (permit number 2012.III.02.020). Material for the transcriptomic analysis was collected from 22 warmblood mares, and used in previous studies [38][39][40]; material for the in vitro experiments was harvested from an additional 5 mares. Mares were between 4 and 15 years of age and maintained on pasture with ad libitum access to grass, hay and water.…”

Section: Animals and Tissue Collectionmentioning

confidence: 99%

“…For example, the embryonic disc (day 14) and embryo proper (day 19) are smaller and less well developed in conceptuses resulting after transfer of day 8 horse embryos into a 5-day negatively asynchronous recipient compared to a synchronous recipient [38]. In addition, the pregnancy stage-related up-regulation in mRNA expression for a range of imprinted genes, glucose and amino acid transporters is delayed in conceptuses that develop in a negatively asynchronous uterus [38][39][40].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Asynchronous Embryo Transfer Followed by Comparative Transcriptomic Analysis of Conceptus Membranes and Endometrium Identifies Processes Important to the Establishment of Equine Pregnancy

Gibson¹,

Ruijter-Villani²,

Bauersachs³

et al. 2020

Preprint

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Pre-implantation horse conceptuses require nutrients and signals from histotroph, the composition of which is regulated by luteal progesterone and conceptus-secreted factors. To distinguish progesterone and conceptus effects we shortened the period of endometrial progesterone-priming by asynchronous embryo transfer. Day 8 embryos were transferred to synchronous (day 8) or asynchronous (day 3) recipients, and RNA sequencing was performed on endometrium and conceptuses recovered 6 and 11 days later (embryo days 14 and 19). Asynchrony resulted in many more differentially expressed genes (DEGs) in conceptus membranes (3473) than endometrium (715). Gene ontology analysis identified upregulation in biological processes related to organogenesis and preventing apoptosis in synchronous conceptuses on day 14, and in cell adhesion and migration on day 19. Asynchrony also resulted in large numbers of DEGs related to 'extracellular exosome'. In endometrium, genes involved in immunity, the inflammatory response, and apoptosis regulation were upregulated during synchronous pregnancy and, again, many genes related to extracellular exosome were differentially expressed. Interestingly, only 14 genes were differentially expressed in endometrium recovered 6 days after synchronous versus 11 days after asynchronous transfer (day 14 recipient in both). Among these, KNG1 and IGFBP3were consistently up-regulated in synchronous endometrium. Furthermore bradykinin, an active peptide cleaved from KNG1, stimulated prostaglandin release by cultured trophectoderm cells. The horse conceptus thus responds to a negatively asynchronous uterus by extensively adjusting its transcriptome, whereas the endometrial transcriptome is modified only subtly by a more advanced conceptus. the donor (synchronous; n=10), or 5 days after the donor mare (asynchronous; n=10). Conceptuses were then recovered on day 14 or day 19 of development (n=5 per group). Day 14 conceptuses were recovered 6 days after ET by uterine lavage via a sterile endotracheal tube, and day 19 conceptuses were recovered 11 days after ET using an endoscopically-guided net, after puncture of the membranes and aspiration of the yolk-sac fluid using a sharpened PTFE catheter [41]. Following conceptus recovery, an endometrial biopsy was collected, using alligator forceps (141965; Jørgen Kruuse A/S, Langeskov, Denmark), from the base of one or other uterine horn for day 14 conceptuses (mobile phase), or from the site of conceptus apposition for day 19 conceptuses (fixed location; n=5 per group) under videoendoscopic guidance. For conceptus membrane culture, pregnancy status was monitored by transrectal ultrasonography from day 14 after ovulation, and conceptuses were recovered on day 19 by uterine lavage via a sterile endotracheal tube (n=5 conceptuses).Conceptuses and endometrial biopsies were washed with sterile 0.9% NaCl. Under a stereomicroscope, the blastocyst capsule was removed from the conceptuses and the embryonic disc region, or embryo proper, were dissected from the conceptus membranes...

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Leucine improves dietary protein use efficiency by regulating protein synthesis by activating amino acid transporters and the mTORC1 pathways in Chinese mitten crab (Eriocheir sinensis)

Song,

Yu,

et al. 2024

Aquaculture

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Amino acid transporter expression in the endometrium and conceptus membranes during early equine pregnancy

Cited by 15 publications

References 37 publications

Asynchronous Embryo Transfer Followed by Comparative Transcriptomic Analysis of Conceptus Membranes and Endometrium Identifies Processes Important to the Establishment of Equine Pregnancy

Asynchronous Embryo Transfer Followed by Comparative Transcriptomic Analysis of Conceptus Membranes and Endometrium Identifies Processes Important to the Establishment of Equine Pregnancy

Asynchronous Embryo Transfer Followed by Comparative Transcriptomic Analysis of Conceptus Membranes and Endometrium Identifies Processes Important to the Establishment of Equine Pregnancy

Leucine improves dietary protein use efficiency by regulating protein synthesis by activating amino acid transporters and the mTORC1 pathways in Chinese mitten crab (Eriocheir sinensis)

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