Amino acid substitutions of acetylcholinesterase associated with carbofuran resistance in <i>Chilo suppressalis</i>

Chang, Cheng‐Nan; Cheng, Xuan; Huang, Xinyi; Dai, Shu‐Mei

doi:10.1002/ps.3770

Cited by 13 publications

(13 citation statements)

References 34 publications

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“…The G227A mutation of ace2, which encodes the AChE targeted by OP and carbamate insecticides in the Drosophilidae and Muscidae (Weill et al 2002), confers resistance to OP in Drosophila melanogaster and Musca domestica (Walsh et al 2001). The A201S mutation of ace1 has been identified in OP-and carbamate-resistant strains of A. gossypii (Andrews et al 2004;Li & Han 2004), C. suppressalis (Jiang et al 2009;Chang et al 2014) and T. urticae (Khajehali et al 2010).…”

Section: Mutations At the Ace1 Locus Confer Resistance To Chlorpyrifosmentioning

confidence: 99%

Temperature‐sensitive fitness cost of insecticide resistance in Chinese populations of the diamondback moth Plutella xylostella

Zhang

Jing

et al. 2015

Molecular Ecology

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Alleles conferring a higher adaptive value in one environment may have a detrimental impact on fitness in another environment. Alleles conferring resistance to pesticides and drugs provide textbook examples of this trade-off as, in addition to conferring resistance to these molecules, they frequently decrease fitness in pesticide/drug-free environments. We show here that resistance to chlorpyrifos, an organophosphate (OP), in Chinese populations of the diamondback moth, Plutella xylostella, is conferred by two mutations of ace1 - the gene encoding the acetylcholinesterase enzyme targeted by OPs - affecting the amino acid sequence of the corresponding protein. These mutations were always linked, consistent with the segregation of a single resistance allele, ace1R, carrying both mutations, in the populations studied. We monitored the frequency of ace1R (by genotyping more than 20 000 adults) and the level of resistance (through bioassays on more than 50 000 individuals) over several generations. We found that the ace1R resistance allele was costly in the absence of insecticide and that this cost was likely recessive. This fitness costs involved a decrease in fecundity: females from resistant strains laid 20% fewer eggs, on average, than females from susceptible strains. Finally, we found that the fitness costs associated with the ace1R allele were greater at high temperatures. At least two life history traits were involved: longevity and fecundity. The relative longevity of resistant individuals was affected only at high temperatures and the relative fecundity of resistant females - which was already affected at temperatures optimal for development - decreased further at high temperatures. The implications of these findings for resistance management are discussed.

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Section: Mutations At the Ace1 Locus Confer Resistance To Chlorpyrifosmentioning

confidence: 99%

Temperature‐sensitive fitness cost of insecticide resistance in Chinese populations of the diamondback moth Plutella xylostella

Zhang

Jing

et al. 2015

Molecular Ecology

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“…Chemical control is still the major approach used to protect rice from damage by the rice stem borer. Unfortunately, C. suppressalis has developed high resistance to organophosphate and nereistoxin insecticides due to their sustained application in the field, and the estimated cost of controlling this pest is approximately US$160 million annually (Wu et al, 2013a;Chang et al, 2014;Su et al, 2014). However, insecticides are not always effective due to the narrow window for damage control between hatching and penetration into the plant stem of rice stem larvae (Wang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression profiles of nicotinic acetylcholine receptors in the rice striped stem borer, Chilo suppressalis (Lepidoptera: Crambidae)

Teng

et al. 2016

Insect Science

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Nicotinic acetylcholine receptors (nAChRs) are members of the cys-loop ligandgated ion channel (cysLGIC) superfamily, mediating fast synaptic cholinergic transmission in the central nervous system in insects. Insect nAChRs are the molecular targets of economically important insecticides, such as neonicotinoids and spinosad. Identification and characterization of the nAChR gene family in the rice striped stem borer, Chilo suppressalis, could provide beneficial information about this important receptor gene family and contribute to the investigation of the molecular modes of insecticide action and resistance for current and future chemical control strategies. We searched our C. suppressalis transcriptome database using Bombyx mori nAChR sequences in local BLAST searches and obtained the putative nAChR subunit complementary DNAs (cDNAs) via reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. Similar to B. mori, C. suppressalis possesses 12 nAChR subunits, including nine α-type and three β-type subunits. Quantitative RT-PCR analysis revealed the expression profiles of the nAChR subunits in various tissues, including the brain, subesophageal ganglion, thoracic ganglion, abdominal ganglion, hemocytes, fat body, foregut, midgut, hindgut and Malpighian tubules. Developmental expression analyses showed clear differential expression of nAChR subunits throughout the C. suppressalis life cycle. The identification of nAChR subunits in this study will provide a foundation for investigating the diverse roles played by nAChRs in C. suppressalis and for exploring specific target sites for chemicals that control agricultural pests while sparing beneficial species.

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“…Diverse works correlate the resistance to pesticides with the increase of enzymatic activity, attributing it to punctual mutations, genic duplications and mutations in the target molecule (Mutero et al, 1994;Villatte et al, 2000;Vontas et al, 2001;Vontas et al, 2002;Ortelli et al, 2003;Menozzi et al, 2004;Revuelta et al, 2009;Ying et al, 2013;Chang et al, 2014;Zhao et al, 2014;Xi et al, 2015).…”

Section: Metabolic Resistancementioning

confidence: 99%

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Rojas

Bermudez

Cambero-Campos

et al. 2017

rbio

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Amino acid substitutions of acetylcholinesterase associated with carbofuran resistance in Chilo suppressalis

Abstract: Amino acid substitutions identified in the AChE of C. suppressalis are associated with changes in AChE kinetics and its insensitivity to carbofuran. These observations are helpful for rapid monitoring, prediction and management of OP and CB resistance in the field.

Cited by 13 publications

References 34 publications

Temperature‐sensitive fitness cost of insecticide resistance in Chinese populations of the diamondback moth Plutella xylostella

Temperature‐sensitive fitness cost of insecticide resistance in Chinese populations of the diamondback moth Plutella xylostella

Molecular characterization and expression profiles of nicotinic acetylcholine receptors in the rice striped stem borer, Chilo suppressalis (Lepidoptera: Crambidae)

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