“…Many other cytochromes c have an identical sequence (-Gly-Arg-Lys-Thr-Gly-) to Asterias in the region 37-41, but no similar anomalous cleavage has been reported [1,2], although partial hydrolysis at threonine-40 was reported in human [13]. However, the activity of TPCK-trypsin at tyrosine-97 of cytochrome c is quite common and may be due to a less than complete ethanolic denaturation coupled with the position of this residue in the three-dimensional structure of the molecule [14,15].…”