Amino acid sequence of the AhR1 ligand‐binding domain predicts avian sensitivity to dioxin like compounds: In vivo verification in European starlings

Eng, Margaret L.; Elliott, John E.; Jones, Stephanie P.; Williams, Tony; Drouillard, Ken G.; Kennedy, Sean W.

doi:10.1002/etc.2743

Cited by 6 publications

(17 citation statements)

References 47 publications

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“…Dose–response curves for early life stage mortality that were predicted using EC Threshold or EC50 as input variable in the corresponding qAOPs were validated against 25 experimental dose–response curves from the published literature that reported measured concentrations and percentage of mortality for each dose (Brunstrom and Reutergardh 1986; Brunstrom and Lund 1988; Brunstrom 1989, 1990; Spitsbergen et al 1991; Walker et al 1994; Walker and Peterson 1994; Powell et al 1996, 1998; Elonen et al 1998; Hoffman et al 1998; Cohen‐Barnhouse et al 2011; Eng et al 2014, 2017; Tillitt et al 2017). These 25 scenarios represent 9 species of birds, 7 species of fish, and 5 different DLCs.…”

Section: Methodsmentioning

confidence: 99%

Predicting Early Life Stage Mortality in Birds and Fishes from Exposure to Low‐Potency Agonists of the Aryl Hydrocarbon Receptor: A Cross‐Species Quantitative Adverse Outcome Pathway Approach

Doering

Dubiel

Wiseman

2020

Enviro Toxic and Chemistry

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Dioxin-like compounds (DLCs) cause early life stage mortality of vertebrates through activation of the aryl hydrocarbon receptor (AhR). A prior study developed a cross-species quantitative adverse outcome pathway (qAOP) which can predict full dose-response curves of early life stage mortality for any species of bird or fish exposed to DLCs using the species-and chemical-specific 50% effect concentration (EC50) from an in vitro AhR transactivation assay with COS-7 cells. However, calculating a reliable EC50 for input into this qAOP requires the maximal response of the concentration-response curve to be known, which is not always possible for low-potency agonists, such as some polychlorinated biphenyls (PCBs). To enable predictions for these low-potency agonists, the present study revised this qAOP to use the effect concentration threshold (EC Threshold) from the in vitro AhR transactivation assay as input. Significant linear relationships were demonstrated between EC Threshold and the dose to cause 0, 10, 50, or 100% mortality among early life stages of 3 species of birds and 7 species of fish for 4 DLCs: 2,3,7,8-tetrachlorodibenzo-p-dioxin, PCB 126, PCB 77, and PCB 105. These 4 linear relationships were combined to form the revised qAOP. This qAOP using the EC Threshold enables prediction of experimental dose-response curves for lower-potency agonists to within an order of magnitude on average, but the prior qAOP using EC50 predicts experimental dose-response curves for higher-potency agonists with greater accuracy.

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Section: Methodsmentioning

confidence: 99%

Predicting Early Life Stage Mortality in Birds and Fishes from Exposure to Low‐Potency Agonists of the Aryl Hydrocarbon Receptor: A Cross‐Species Quantitative Adverse Outcome Pathway Approach

Doering

Dubiel

Wiseman

2020

Enviro Toxic and Chemistry

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“…Other birds classified as semisensitive were Tachycineta bicolor , Troglodytes aedon, and Phalacrocorax sp. Although experimental studies focusing on the reproductive effects of PCB residues in eggs have been performed on species classified as highly sensitive (e.g., European starling ( Sturnus vulgaris ) or the gray catbird ( Dumetella carolinensis )), , field monitoring data on these species was grossly lacking. This implies that our SSDs most likely underestimate the effects of dioxin-like PCBs toward birds.…”

Section: Discussionmentioning

confidence: 99%

Deriving Field-Based Ecological Risks for Bird Species

Hoondert

Hilbers

Hendriks

et al. 2018

Environ. Sci. Technol.

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Ecological risks (ERs) of pollutants are typically assessed using species sensitivity distributions (SSDs), based on effect concentrations obtained from bioassays with unknown representativeness for field conditions. Alternatively, monitoring data relating breeding success in bird populations to egg concentrations may be used. In this study, we developed a procedure to derive SSDs for birds based on field data of egg concentrations and reproductive success. As an example, we derived field-based SSDs for p,p′-DDE and polychlorinated biphenyls (PCBs) exposure to birds. These SSDs were used to calculate ERs for these two chemicals in the American Great Lakes and the Arctic. First, we obtained field data of p,p′-DDE and PCBs egg concentrations and reproductive success from the literature. Second, these field data were used to fit exposure-response curves along the upper boundary (right margin) of the response’s distribution (95th quantile), also called quantile regression analysis. The upper boundary is used to account for heterogeneity in reproductive success induced by other external factors. Third, the species-specific EC10/50s obtained from the field-based exposure-response curves were used to derive SSDs per chemical. Finally, the SSDs were combined with specific exposure data for both compounds in the two areas to calculate the ER. We found that the ERs of combined exposure to these two chemicals were a factor of 5–35 higher in the Great Lakes compared to Arctic regions. Uncertainty in the species-specific exposure-response curves and related SSDs was mainly caused by the limited number of field exposure-response data for bird species. With sufficient monitoring data, our method can be used to quantify field-based ecological risks for other chemicals, species groups, and regions of interest.

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“…European starlings are particularly useful as a study species because their ecology and physiology are well studied, they can be attracted to and reared in nest boxes, and they are amenable to captivity. Starlings have also been used widely in ecotoxicology studies and have recently been identified as a highly sensitive species given the structural affinity of their aryl hydrocarbon receptor to bind strongly to dioxin‐like compounds, including certain hydroxylated PCBs .…”

Section: Discussionmentioning

confidence: 99%

Latent cognitive effects from low‐level polychlorinated biphenyl exposure in juvenile European starlings (Sturnus vulgaris)

Zahara

Michel

Flahr

et al. 2015

Enviro Toxic and Chemistry

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Ecotoxicology research on polychlorinated biphenyl (PCB) mixtures has focused principally on short-term effects on reproduction, growth, and other physiological endpoints. Latent cognitive effects from early life exposure to low-level PCBs were examined in an avian model, the European starling (Sturnus vulgaris). Thirty-six birds, divided equally among 4 treatment groups (control = 0 µg, low = 0.35 µg, intermediate = 0.70 µg, and high = 1.05 µg Aroclor 1254/g body weight), were dosed 1 d through 18 d posthatch, then tested 8 mo to 9 mo later in captivity in an analog to an open radial arm maze. Birds were subject to 4 sequential experiments: habituation, learning, cue selection, and memory. One-half of the birds did not habituate to the test cage; however, this was not linked to a treatment group. Although 11 of the remaining 18 birds successfully learned, only 1 was from the high-dosed group. Control and low-dosed birds were among the only treatment groups to improve trial times throughout the learning experiment. High-dosed birds were slower and more error-prone than controls. Cue selection (spatial or color cues) and memory retention were not affected by prior PCB exposure. The results indicate that a reduction in spatial learning ability persists among birds exposed to Aroclor 1254 during development. This may have implications for migration ability, resource acquisition, and other behaviors relevant for fitness.

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Amino acid sequence of the AhR1 ligand‐binding domain predicts avian sensitivity to dioxin like compounds: In vivo verification in European starlings

Cited by 6 publications

References 47 publications

Predicting Early Life Stage Mortality in Birds and Fishes from Exposure to Low‐Potency Agonists of the Aryl Hydrocarbon Receptor: A Cross‐Species Quantitative Adverse Outcome Pathway Approach

Predicting Early Life Stage Mortality in Birds and Fishes from Exposure to Low‐Potency Agonists of the Aryl Hydrocarbon Receptor: A Cross‐Species Quantitative Adverse Outcome Pathway Approach

Deriving Field-Based Ecological Risks for Bird Species

Latent cognitive effects from low‐level polychlorinated biphenyl exposure in juvenile European starlings (Sturnus vulgaris)

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