1976
DOI: 10.1021/bi00652a005
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Amino acid sequence of rabbit light chains: variable region of a light chain from a homogeneous immunoglobulin raised by streptococcal immunization

Abstract: The variable region sequence has been determined for the light chain (L) from a rabbit homogeneous immunoglobulin (3547) produced by immunization with group A streptococcal vaccine. Unlike most immunoglobulins produced by these vaccines, this immunoglobulin had no binding activity for the group A polysaccharide nor for any of a wide range of streptococcal cell components tested, nor did it have binding activity for rabbit IgG. Tryptic digestion of the citraconylated L chain and acid hydrolysis of the aspartyl-… Show more

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Cited by 13 publications
(6 citation statements)
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“…The only exception was antiserum BS-4432, where the major clonotype (isoelectric point 6.1) did not bind Av-CHO. Similar observations were previously reported showing that dominant clonotypes elicited by immunization with streptococcal vaccines are not always group polysaccharide-specific antibodies (24,25). It ap- pears that a substantial number of clonotypes expressed at a low quantitative level exhibited a high antigen binding capacity, for example, the dominant clonotype of BS-4433 (pH 6.7-7.0) was compared with the discrete clonotype focusing at pH 7.5-7.9.…”
Section: Response Patterns Of Basilea Rabbits To the Streptococcal Avsupporting
confidence: 83%
“…The only exception was antiserum BS-4432, where the major clonotype (isoelectric point 6.1) did not bind Av-CHO. Similar observations were previously reported showing that dominant clonotypes elicited by immunization with streptococcal vaccines are not always group polysaccharide-specific antibodies (24,25). It ap- pears that a substantial number of clonotypes expressed at a low quantitative level exhibited a high antigen binding capacity, for example, the dominant clonotype of BS-4433 (pH 6.7-7.0) was compared with the discrete clonotype focusing at pH 7.5-7.9.…”
Section: Response Patterns Of Basilea Rabbits To the Streptococcal Avsupporting
confidence: 83%
“…A homogeneous antibody with allotype a3b4 (4539 Ab) was isolated from the transfusion plasma by DEAEcellulose chromatography and chromatography on a Group C carbohydrate immunoadsorbent column (7). Amino acid sequence studies on the light chain of 4539 Ab have been carried out using a strategy described elsewhere (8). Constant-region sequence variation was first observed upon sequence determination of a peptide (residues 110-207) isolated after acid cleavage (9) of the tryptic fragment encompassing residues 62-207.…”
Section: Methodsmentioning
confidence: 99%
“…Except for a threonine residue at position 50, which is also found in 2717, positions 50-52 in the Xp-1 light chain are different from all other published rabbit light-chain sequences (Jaton, 1974a,b;Chen et a]., 1975;Margolies et al, 1975;Thunberg and Kindt, 1976). Positions 50-56 are hypervariable in the light chains of other species (Wu and Kabat, 1970).…”
Section: Alaasp~evalmetthrglnthrproalaservalsergluprovalglyglythrvaltmentioning
confidence: 79%