Amino acid sequence of EC2 domain in CD81 is highly conserved in Japanese subjects

Ito, Nobuyuki; Kawata, Sumio; Tamura, Shinji; Nakajima, Hitoshi; Kiso, Shinichi; Saeki, Ayuko; Oue, Takanori; Hanafusa, Toshiaki; Matsuzawa, Yūji

doi:10.1002/hep.510310245

Cited by 4 publications

(1 citation statement)

References 5 publications

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“…Notably, our study showed that CD81-RNA expression levels were similar for HCC and noncancerous portions of the liver, indicating that low HCV level in HCC is not attributable to quantity differences of CD81's cytoplasmic RNA. Our data together with the lack of CD81 polymorphism in HCC patients [Ito et al, 2000;Itakura et al, 2001] argue against the hypothesis that HCV infection resistance is linked to modified CD81 expression. According to our current data, CD81 gene expression is not modulated by persistent HCV infection and vice versa.…”

Section: Discussioncontrasting

confidence: 61%

Variation of hepatitis C virus load, hypervariable region 1 quasispecies and CD81 hepatocyte expression in hepatocellular carcinoma and adjacent non‐cancerous liver

Young¹,

Lin²,

Hsiao³

et al. 2002

Journal of Medical Virology

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Hepatitis C virus (HCV) infection is etiologically associated with the development of hepatocellular carcinoma (HCC) worldwide. HCV has been reported to exist and replicate in both HCC and adjacent non-cancerous liver tissue, but limited information was available on HCV viral load and quasispecies composition in HCC relative to adjacent non-cancerous hepatocytes. Previous study has also suggested CD81, a surface hepatocyte protein, as a receptor for HCV. To clarify the above, HCV-RNA and CD81-RNA titers in 20 paired hepatectomized liver and serum were quantitatively measured by chemiluminescent RT-cPCR. Hypervariable region 1 (HVR-1) variations of parallel specimens were analyzed after subcloning in 6 patients. HCV-RNA levels in serum and non-cancerous liver were markedly higher for HCV genotype 1 than genotype non-1. HCV levels were markedly higher in non-cancerous liver than in HCC (P = 0.001) in a genotype-independent manner, with a mean ratio of 56:1 for non-cancerous tissue to HCC. Both non-cancerous and HCC tissues had the same level of CD81-RNA expression, which was not linked to HCV load. HCV-RNA quantity in both HCC and non-cancerous liver correlated with the number of HVR-1 quasispecies in the tissue, and distinct HVR-1 subclones existed.

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Section: Discussioncontrasting

confidence: 61%

Variation of hepatitis C virus load, hypervariable region 1 quasispecies and CD81 hepatocyte expression in hepatocellular carcinoma and adjacent non‐cancerous liver

Young¹,

Lin²,

Hsiao³

et al. 2002

Journal of Medical Virology

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CD81 nucleotide mutation in hepatocellular carcinoma and lack of CD81 polymorphism in patients at stages of hepatitis C virus infection

et al. 2000

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Mechanisms determining the chronicity or the pattern of clinical course of hepatitis C virus (HCV) infections have not been clarified. Recently, CD81 was reported to bind the E2 protein of HCV and was suggested to function as a cellular receptor for HCV. Accordingly, the hypothesis was examined that CD81 polymorphism, if it exists, might correlate with certain clinical courses of HCV infection. CD81 cDNA sequences were determined from peripheral blood mononuclear cells (PBMCs). Twenty-four Japanese subjects were enrolled initially as follows: patients with chronic hepatitis C without cirrhosis (n = 3), patients with cirrhosis (n = 3), patients with cirrhosis complicated by hepatocellular carcinoma (HCC) (n = 3), patients with persistent HCV viremia without ALT elevation (n = 3), those with positive anti-HCV antibodies without evidence of HCV viremia (n = 3), and healthy volunteers (n = 9). In all PBMCs samples analyzed, no polymorphism was found in the CD81 cDNA sequence. The sequence was different, however, from the one reported previously at three nucleotide positions: a transversion to thymine instead of cytosine at nt 1130, a deletion at nt 1206, and a guanine insertion at nt 71. Subsequently, CD81 cDNA sequences from PBMCs and HCC tissue were compared among the other 6 patients with chronic hepatitis C bearing HCC. A comparative study of the CD81 sequences from HCC and PBMCs revealed that various nucleotide mutations existed only in the HCC samples in 3 out of 6 patients. Several mutations in the 3' non-coding region of CD81 cDNA were observed exclusively in HCC tissue suggesting its possible role in hepatocarcinogenesis. Because of the absence of polymorphisms, however, CD81 is unlikely to affect the progression of chronic hepatitis C in terms of chronicity, hepatitis activity, or disease stage.

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Genetic variations in humans associated with differences in the course of hepatitis C

Saito

Ji²,

Shinzawa

et al. 2004

Biochemical and Biophysical Research Communications

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Amino acid sequence of EC2 domain in CD81 is highly conserved in Japanese subjects

Cited by 4 publications

References 5 publications

Variation of hepatitis C virus load, hypervariable region 1 quasispecies and CD81 hepatocyte expression in hepatocellular carcinoma and adjacent non‐cancerous liver

Variation of hepatitis C virus load, hypervariable region 1 quasispecies and CD81 hepatocyte expression in hepatocellular carcinoma and adjacent non‐cancerous liver

CD81 nucleotide mutation in hepatocellular carcinoma and lack of CD81 polymorphism in patients at stages of hepatitis C virus infection

Genetic variations in humans associated with differences in the course of hepatitis C

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