Amino acid profile and enhancement of the enzymatic hydrolysis of fermented shrimp carotenoproteins

Assessment of Functional Lipid Constituents of Red (Aristaeomorpha foliacea) and Pink (Parapenaeus longirostris) Shrimps

“…The last-mentioned compounds showed similar absorption spectra with trans-astaxanthin, demonstrating astaxanthinderived compounds like astaxanthin esters [12]. Crustaceans, such as shrimps, contain astaxanthin as their main pigment, which is mainly formed from beta-carotene or zeaxanthin via the oxidative conversion [27,28].…”

Optimization of enzymatic hydrolysis of shrimp waste for recovery of antioxidant activity rich protein isolate

“…In most of the studies on isolation of carotenoprotein from shrimp byproducts by enzymatic technique, focus was on increased yield of protein and maximizing its recovery (Klomklao et al 2009;Holanda and Netto 2006;Armenta and Guerrero-Legarreta 2009;Cao et al 2008;. Protein isolates as well as carotenoids are known to possess strong antioxidant activity.…”

Influence of pectin on intestinal digestion of chromogens in steers

“…The mechanisms for interaction of pectin with ␤-carotene are uncertain. However, it is likely that ␤-carotene is captured within the gel phase, stabilized by hydrophobic interactions between the free hydroxyl substituents and the methyl ␤-ionone ring (Armenta and Guerrero, 2009).…”

“…Sample extractions of ␤-carotene, chlorophyll-a and -b, were performed using a modification of the technique of Bruinsma (1963): (1) weighed samples of alfalfa hay (0.5 g), duodenal chyme (5.0 g) and feces (0.5 g) were placed in 50 mL amber flasks; (2) 20 mL of acetone and 0.01% of a mixture of BHA: BHT (1:1) was added into of each flask; (3) flasks were shaken at 200 rpm orbital shaking incubator (Lab-Line, Melrose Park, Illinois, USA) for 3 h at room temperature, avoiding contact with natural light; (4) samples were filtered on Whatman # 2 paper, washing with 20 mL of petroleum ether; (5) filtrate was stirred continuously for approximately 5 min; (6) after phase separation, the lower phase was discarded, and this procedure was repeated 2-3 times; (7) acetone was removed with 2 or 3 successive washes with distilled water; (8) 10 mL of 40% NaOH was added to acetone extract and shaken; (9) the bottom layer bottom was removed and washed 1-3 times with distilled water to remove the NaOH (confirmed with 3 drops of phenolphthalein); (10) Samples were then washed 1-3 times with 20 mL of 10% NaSO 4 ; 11) the bottom layer was extracted and placed in 50 mL volumetric flasks and gauged with acetone/petroleum ether/water (85:10:5, vol/vol/vol); 12) samples were filtered through 0.45 micron Acro-disks (Gelman Acrodisc Krackeler Scientific, Albany, USA). Filtrate was analyzed for ␤-carotene, chlorophyll-a and -b according to HPLC procedures of Armenta andGuerrero (2009) andFranco et al (2010).…”