Amino Acid-Permeable Anion Channels in Early Mouse Embryos and Their Possible Effects on Cleavage1

Sonoda, M.; Okamoto, Fujio; Kajiya, Hiroshi; Inoue, Yuichi; Honjo, Ko; Sumii, Yoshinari; Kawarabayashi, Tatsuhiko; Okabe, Koji

doi:10.1095/biolreprod.102.007088

Cited by 12 publications

(14 citation statements)

References 20 publications

(31 reference statements)

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“…In the 1-, 4-, and 8-cell stages, the DIDS-sensitive current constituted all the Cl K -sensitive current. The electrophysiological characteristics of this outwardly rectifying Cl K current were similar to that of a current described previously in the preimplantation mouse embryo under both isotonic and hypotonic conditions (Kolajova & Baltz 1999, Kolajova et al 2001, Sonoda et al 2003. In the blastocyst, this DIDS-sensitive current was present in both the TE and the ICM.…”

Section: Discussionsupporting

confidence: 84%

“…mRNAs encoding all the Clcn channels were detected throughout development extending a previous report of Clcn expression in zygotes (Sonoda et al 2003). Two types of ClC activities were detected using whole-cell voltage clamp methods.…”

Section: Discussionsupporting

confidence: 75%

“…In mouse embryos, VSOAC is only present in the oocyte, 1-, and 2-cell stages in which its activation by hypotonicity is cell cycle dependent (Kolajova & Baltz 1999, Kolajova et al 2001. DIDS prevents cell proliferation and development of mouse embryos (Sonoda et al 2003, Li et al 2009), suggesting that CLCN3 may play a role in regulation of the resting cell volume, and thereby cell proliferation, throughout development.…”

Section: Discussionmentioning

confidence: 99%

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Changing expression of chloride channels during preimplantation mouse development

et al. 2013

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Plasma membrane chloride channels (ClCs) play important roles in a broad range of cellular processes including cell volume regulation, proliferation, and transepithelial transport, all of which are critical during preimplantation embryonic development. In this study, the molecular and functional expression of voltage-gated ClCs was analyzed throughout preimplantation development of the mouse conceptus. mRNA transcripts for all Clcn genes were detected. Only Clcn1 mRNA showed differential expression in the blastocyst, being detected in the trophectoderm but not in the inner cell mass. CLCN3 protein was detected at low levels in the cytoplasm and plasma membrane in 4-cell embryos and was localized to the apical plasma membrane of the trophoblasts in the blastocyst. Whole-cell patchclamp recordings demonstrated the presence of a DIDS-sensitive, outwardly rectifying Cl K current throughout development, with this conductance being large at the 1-cell, morula and blastocyst stages. A second DIDS-insensitive Cl K current, which was inactivated by membrane depolarization, was present in cells differentiating into the trophoblast lineage and during blastocyst expansion. Inhibition of the DIDS-sensitive current and the DIDS-insensitive current, with 9-AC, prevented blastocyst expansion.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

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Changing expression of chloride channels during preimplantation mouse development

et al. 2013

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“…Anion channels, such as Cl -channels, play several roles, but the main role is the regulation of cell volume in somatic cells and in early preimplantation embryos. During embryo culture, the uptake of amino acids was shown to be mediated by Cl -channels in the mouse (Sonoda et al, 2003), and swelling-activated anion current in early mouse embryos was shown to be developmentally regulated and cell-cycle dependent (Kolajova et al, 2001).…”

Section: Mammalsmentioning

confidence: 99%

Ion currents in embryo development

Tosti

Boni

Gallo

2016

Birth Defects Research Pt C

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Ion channels are proteins expressed in the plasma membrane of electrogenic cells. In the zygote and blastomeres of the developing embryo, electrical modifications result from ion currents that flow through these channels. This phenomenon implies that ion current activity exerts a specific developmental function, and plays a crucial role in signal transduction and the control of embryogenesis, from the early cleavage stages and during growth and development of the embryo. This review describes the involvement of ion currents in early embryo development, from marine invertebrates to human, focusing on the occurrence, modulation, and dynamic role of ion fluxes taking place on the zygote and blastomere plasma membrane, and at the intercellular communication between embryo cell stages.Birth Defects Research (Part C) 108:6-18, 2016.V C 2016 Wiley Periodicals, Inc.

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“…Electrophysiological measurements on oocytes and preimplantation embryos have shown that, like most somatic cells, oocytes and early embryos of mouse and human exhibit swelling-activated channel (VRAC or VSOAC) activity (Kolajova and Baltz, 1999;Hammer et al, 2000;Kolajova et al, 2001). In embryos, these channels have been shown to be permeable to a number of organic compounds, including osmolytes such as glycine and taurine (Kolajova and Baltz, 1999;Sonoda et al, 2003), and 1-cell mouse embryos can recover from cell swelling by activating these channels to release intracellular osmolytes including glycine (Seguin and Baltz, 1997;. Although the anion current presumably carried by VRAC in mouse embryos was downregulated during metaphase at the 2-to 4-cell transition, and remained low through the morula stage, swelling-activated efflux of organic osmolytes recovered after metaphase (Kolajova et al, 2001).…”

Section: Recovery From Cell Volume Increase and Release Of Accumulatementioning

confidence: 99%

Cell volume regulation in mammalian oocytes and preimplantation embryos

Baltz

Zhou

2012

Molecular Reproduction Devel

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SUMMARYThe earliest stages of preimplantation embryos are particularly sensitive to increased osmolarity, even within the physiological range. This sensitivity contributed to persistent developmental arrest, even when embryos were cultured in vitro in older, conditioned culture media, and seems to arise when embryos at the 1-and 2-cell stages accumulate inorganic ions used for cell volume homeostasis at too high a level, through activation of coupled Na þ /H þ and HCO 3 À /Cl À exchange. Such accumulation of inorganic ions can be disruptive since, above a certain level, the increased ionic strength disrupts cellular biochemistry and macromolecular functions and alters membrane potential. To counter this, embryos have evolved mechanisms of cell volume regulation that are unique to early preimplantation embryogenesis. The primary role of these is glycine accumulation via the GLYT1 transporter, with a secondary contribution by betaine accumulation via the SIT1 transporter. Independent cell-volume regulation first arises in the oocyte only after ovulation is triggered, when the strong oocyte-zona pellucida adhesion present in germinal vesicle stage oocytes in the ovarian follicle is released and GLYT1 becomes activated to begin accumulating glycine. Open questions still remain regarding how these processes are regulated.Mol. Reprod. Dev. 79: 821-831, 2012. ß

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Amino Acid-Permeable Anion Channels in Early Mouse Embryos and Their Possible Effects on Cleavage1

Cited by 12 publications

References 20 publications

Changing expression of chloride channels during preimplantation mouse development

Changing expression of chloride channels during preimplantation mouse development

Ion currents in embryo development

Cell volume regulation in mammalian oocytes and preimplantation embryos

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