1974
DOI: 10.1002/bms.1200010206
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Amino acid mixture analysis by mass spectrometry in the form of their dimethylaminomethylene methyl esters

Abstract: Abstrae-The amino acids, arginine. citrulline, cysteine, cystine, histidine, hydroxyproline, rnethionine. ornithine, proline. serine, threonine. tryptophan and tyrosine. have been analysed in the form of their N-(N',N'-dimethylaminomethylene) methyl esters. formed in a single step by reaction of the free amino acid or its hydrochloride with N,N-dimethylformamide dimethylacetal in acetonitrile/methanol (2 : 1). A reaction time of 15 minutes at 100 "C ensures complete derivatization, the derivatives being volati… Show more

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Cited by 24 publications
(12 citation statements)
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“…Isotopic enrichment in glutamate, aspartate, and alanine was monitored using ratios of ions at m/z 433/432, 419/418, and 261/260, respectively. Enrichment in [2-15 N]glutamine was determined by monitoring m/z 259/258, and [5- citrulline was determined using the N-dimethylaminomethylene methyl ester derivative (38). Briefly, an aliquot of a tissue extract was purified via an AG-50 (H ϩ ; 100 -200 mesh; 0.5 ϫ 2.5 cm) column, dried, and then converted into N-dimethylaminomethylene methyl ester derivatives (38).…”
Section: Gc-ms Methodology and Determination Of 15 N-labeled Metabolimentioning
confidence: 99%
See 1 more Smart Citation
“…Isotopic enrichment in glutamate, aspartate, and alanine was monitored using ratios of ions at m/z 433/432, 419/418, and 261/260, respectively. Enrichment in [2-15 N]glutamine was determined by monitoring m/z 259/258, and [5- citrulline was determined using the N-dimethylaminomethylene methyl ester derivative (38). Briefly, an aliquot of a tissue extract was purified via an AG-50 (H ϩ ; 100 -200 mesh; 0.5 ϫ 2.5 cm) column, dried, and then converted into N-dimethylaminomethylene methyl ester derivatives (38).…”
Section: Gc-ms Methodology and Determination Of 15 N-labeled Metabolimentioning
confidence: 99%
“…Enrichment in [2-15 N]glutamine was determined by monitoring m/z 259/258, and [5- citrulline was determined using the N-dimethylaminomethylene methyl ester derivative (38). Briefly, an aliquot of a tissue extract was purified via an AG-50 (H ϩ ; 100 -200 mesh; 0.5 ϫ 2.5 cm) column, dried, and then converted into N-dimethylaminomethylene methyl ester derivatives (38). 15 N enrichment in N-acetylglutamate in liver or mitochondrial extracts were determined using GC-MS and an isotope dilution approach (12).…”
Section: Gc-ms Methodology and Determination Of 15 N-labeled Metabolimentioning
confidence: 99%
“…The residue recovered from the Dowex 50 column was transferred to a 1-ml reaction vial with ethanol. The ethanol was removed under a stream of nitrogen and the dimethylaminomethylene ethyl ester derivative of the amino acid was prepared by heating the residue in a mixture of 100 Ml of dimethylformamide diethylacetal, 60 Ml of acetonitrile, and 30 Ml of ethanol at 1000 for 15 min (21,22).…”
Section: Methodsmentioning
confidence: 99%
“…(N, N)-Dimethylformamide dimethyl acetal (DMFDMA) derivatization (modified from Horman and Hesford, 1974;Thenot and Horning, 1972). The dried hydrolysate was dissolved in 100 +L methanol.…”
Section: Preparation Of Amino Acid Derivativesmentioning
confidence: 99%