Amino acid metabolism in euryhaline bivalves: Regulation of glycine accumulation in ribbed mussel gills

Ellis, Lehman L.; Burcham, James M.; Paynter, Kennedy T.; Bishop, Stephen H.

doi:10.1002/jez.1402330303

Cited by 25 publications

(10 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As amino acids are indispensable for protein biosynthesis in the host, the sparse presence of amino acid synthesis-related genes in B. azoricus (even when considering the incompleteness of the available transcriptome information) may indicate that the bivalve depends on its symbionts for supply with amino acids and cofactors. This putative deficiency in amino acid biosynthetic enzymes seems to be uncommon in non-symbiotic marine mussels as several species are capable of de novo synthesizing most amino acids from TCA pathway intermediates ( Ellis et al. , 1985 ).…”

Section: Resultsmentioning

confidence: 99%

Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis

et al. 2016

View full text Add to dashboard Cite

The hydrothermal vent mussel Bathymodiolus azoricus lives in an intimate symbiosis with two types of chemosynthetic Gammaproteobacteria in its gills: a sulfur oxidizer and a methane oxidizer. Despite numerous investigations over the last decades, the degree of interdependence between the three symbiotic partners, their individual metabolic contributions, as well as the mechanism of carbon transfer from the symbionts to the host are poorly understood. We used a combination of proteomics and genomics to investigate the physiology and metabolism of the individual symbiotic partners. Our study revealed that key metabolic functions are most likely accomplished jointly by B. azoricus and its symbionts: (1) CO2 is pre-concentrated by the host for carbon fixation by the sulfur-oxidizing symbiont, and (2) the host replenishes essential biosynthetic TCA cycle intermediates for the sulfur-oxidizing symbiont. In return (3), the sulfur oxidizer may compensate for the host's putative deficiency in amino acid and cofactor biosynthesis. We also identified numerous ‘symbiosis-specific' host proteins by comparing symbiont-containing and symbiont-free host tissues and symbiont fractions. These proteins included a large complement of host digestive enzymes in the gill that are likely involved in symbiont digestion and carbon transfer from the symbionts to the host.

show abstract

Section: Resultsmentioning

confidence: 99%

Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Under hypoosmotic stress, the intracellular concentration of FAAs decreases as a result of release, while under hyperosmotic stress, the intracellular concentration of FAAs increases as a result of uptake (Zurburg and De Zwaan 1981;Matsushima and Hayashi 1988). The roles of alanine and glycine in osmoregulation, for example, have been studied considerably (Baginski and Pierce 1977;Ellis et al 1985). Yet, none of the observed variations in the seasonal content of AAs appears to have occurred as a result of salinity changes (Fig.…”

Section: Amino Acid Profile and Seasonalitymentioning

confidence: 99%

“…Studies conducted so far provided a general view of the seasonal variations in bivalve biochemistry, but few assess the temporal variations occurring among AAs and FAs. Most studies focusing on AAs in bivalves investigate the effects of salinity change on the levels of free AAs (Allen 1961;Ellis 1985;Sokolowski et al 2003;Kube et al 2007) or the AA composition of shells (Dussart 1983;Goodfriend et al 1997;Barbour Wood et al 2006) or specific soft body parts (i.e., gills and mantle; Trytek and Allen 1980). Few studies report whole-body AA composition of bivalves (Sidwell et al 1979;Fatima 1996;Ö zden and Erkan 2011).…”

Section: Introductionmentioning

confidence: 99%

Temporal dynamics of amino and fatty acid composition in the razor clam Ensis siliqua (Mollusca: Bivalvia)

et al. 2014

View full text Add to dashboard Cite

Few studies have been conducted on the temporal dynamics of both amino acid (AA) and fatty acid (FA) profiles in marine bivalves. We investigated the seasonal variation of these compounds in the pod razor clam Ensis siliqua in relation to food availability, salinity, water temperature and reproductive cycle. AA content varied between 46.94 and 54.67 % dry weight (DW), and the AAs found in greater quantity were glutamic acid, glycine and aspartic acid. FA content varied between 34.02 and 87.94 mg g -1 DW and the FAs found in greater quantity were 16:0 and 22:6n-3. Seasonal trends were observed for AAs and FAs. FAs increased with gametogenesis and decreased with spawning while AA content increased throughout spawning. The effect of increasing temperature and high food availability during the spawning season masked the loss of AAs resulting from gamete release. Still, a comparatively greater increase in the contents of glutamic acid and leucine with spawning indicate their possible involvement in a post-spawning gonad recovery mechanism. A post-spawning decrease in 14:0, 16:0, 16:1n-7, 18:1n-7 and 18:1n-9 is indicative of the importance of these FAs in bivalve eggs. An increase in 18:3n-3, 18:4n-3, 20:1n-9 and 20:2n-6 during gametogenesis suggests their involvement in oocyte maturation. The FA 22:4n-6, while increasing with spawning, appears to play a role in post-spawning gonad recovery. Salinity did not have an effect on the AA composition. None of the environmental parameters measured had an effect on FA composition.

show abstract

“…Osmotic effects on mitochondrial oxidation of osmolytes may be important in cell volume regulation in osmoconforming animals . Mitochondria1 oxidation of various osmotically active amino acids is sensitive to changes in mitochondrial volume (Ballantyne and Storey 1983;Ellis et al 1985;Moyes et al 1986). As the effects of hypotonic incubation can be mimicked by acclimation to dilute seawater (with incubation in isotonic medium), it is unlikely that mitochondrial oxidation responds to mitochondrial volume per se, but rather to some mitochondrial parameter altered by both mitochondrial volume changes and acclimation to dilute seawater .…”

Section: Amino Acid Metabolism In Osmoconformersmentioning

confidence: 99%

A comparison of fuel preferences of mitochondria from vertebrates and invertebrates

Moyes¹,

Suarez²,

Hochachka³

et al. 1990

Can. J. Zool.

View full text Add to dashboard Cite

1990. A comparison of fuel preferences of mitochondria from vertebrates and invertebrates. Can. J. Zool. 68: 1337-1349.Knowledge of tissue-specific mitochondrial properties is important in understanding cellular aerobic energy metabolism. Studies employing isolated mitochondria offer the advantage of direct and controlled manipulation of extramitochondrial conditions, while minimizing disruption of interactions between mitochondrial enzymes, transporters, and membranes. In this review, we compare the oxidative properties of mitochondria isolated from liver, heart, and skeletal muscle of vertebrates and invertebrates. The observed differences between tissues and species in the capacities for mitochondrial oxidation of fatty acids, ketone bodies, pyruvate, and amino acids reflect fundamentally different adaptations for the assimilation, storage, and utilization of metabolic fuels. MOYES, C. D., SUAREZ, R. K., HOCHACHKA, P. W., et BALLANTYNE, J. S. 1990. A comparison of fuel preferences of mitochondria from vertebrates and invertebrates. Can. J. Zool. 68 : 1337-1349. La connaissance des propriCtCs mitochondriales spCcifiques A certains tissus est essentielle A la comprChension du mCtabolisme CnergCtique aCrobie des cellules. Les travaux sur des mitochondries isolCes offrent l'avantage d'une manipulation directe et contr61Ce des conditions extramitochondriales tout en affectant le moins possible les interactions entre les enzymes, les ClCments de transport et les membranes des mitochondries. Dans cette synthkse, nous comparons les propriCtCs oxydatives de mitochondries isolCes du foie, du coeur et du muscle squelettjque de vertCbrCs et d'invertCbrCs. Les diffkrences observCes entre divers tissus et entre diffkrentes especes quant A la capacitC mitochondriale d'oxydation des acides gras, des corps cktoniques, des pyruvates et des acides aminCs sont le reflet des adaptations fondamentalement diffkrentes qui rCgissent l'assimilation, le stockage et l'utilisation des comburants mCtaboliques. [Traduit par la revue]Introduction may provide models that are better suited for the study of Cellular energy production via the oxidation of glucose, fatty acids, ketone bodies, and amino acids requires participation of rnitachondrial transporters, rnitochondrial matrix and membranebound enzymes, and oxidative phosphorylation. Consequently, studies of metabolite oxidation by isolated mitochondria provide insights into the fuel preferences of various tissues. Because studies that make use of isolated mitochondria minimize disruption of enzyme-enzyme, enzyme-membrane, and enzyme-transporter interactions, they are useful in assessing transport capacities, metabolic flux rates, enzyme activities in situ, and regulation of mitochondrial pathways. Such studies may also yield important information about anabolic capacities,

show abstract

Amino acid metabolism in euryhaline bivalves: Regulation of glycine accumulation in ribbed mussel gills

Cited by 25 publications

References 68 publications

Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis

Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis

Temporal dynamics of amino and fatty acid composition in the razor clam Ensis siliqua (Mollusca: Bivalvia)

A comparison of fuel preferences of mitochondria from vertebrates and invertebrates

Contact Info

Product

Resources

About