Amino Acid Incorporation into Protein by Cell-free Extracts of Yeast<sup>*</sup>

Bretthauer, Roger K.; Marcus, Leon; Chaloupka, J.; Halvorson, Harlyn O.; Bock, Robert M.

doi:10.1021/bi00905a029

Cited by 97 publications

(44 citation statements)

References 6 publications

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“…Several workers (Mager, 1960;Kroon, 1963) have reported that chloramphenicol inhibits amino acid incorporation by intact mitochondria from several sources. However von Ehrenstein & Lipmann (1961) ~ So & Davie (1963) and Bretthauer et al (1963) showed that chloramphenicol did not inhibit amino acid incorporation by isolated cytoplasmic ribosomes from animal and yeast cells. It would seem therefore that chloramphenicol ought to cause a specific inhibition of mitochondrial protein synthesis in vivo at low concentrations if it were able to penetrate the cells sufficiently well.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Chloramphenicol on Growth and Mitochondrial Structure of Pythium ultimum

Marchant

Smith²

1968

Journal of General Microbiology

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SUMMARYChloramphenicol was found to inhibit the growth of Pythium ultimum, showing a maximum dose-response at a concentration of IOO ,ug./ml. Mycelium grown in the presence of chloramphenicol IOO ,ug./ml., was devoid of the mitochondrial cytochromes aa, and b but contained increased cytoplasmic cytochrome c. Mitochondria in thin sections of hyphae grown in the presence of chloramphenicol resembled mitochondria in normal hyphae. In negatively stained preparations of isolated normal mitochondria stalked particles were observed on the cristae. These particles could not be demonstrated in mitochondria from hyphae grown in the presence of chloramphenicol I 00 pg. /ml.

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Section: Introductionmentioning

confidence: 99%

The Effect of Chloramphenicol on Growth and Mitochondrial Structure of Pythium ultimum

Marchant

Smith²

1968

Journal of General Microbiology

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“…Similarly, spermine stimulated a poly (U)-directed synthesis of polyphenylalanine in a system derived from L-1210 mouse leukemia cells [29]. A spermidine-stimulating synthesis of phenylalanine in the presence of poly (U) was also demonstrated in cell-free extracts of yeast [30]. Similar effects were also observed with extracts of Helianthus tuberoses [31].…”

Section: (C) Polyamines and Ribosomal Rnamentioning

confidence: 71%

Naturally Occurring Polyamines: Interaction with Macromolecules

Bachrach¹

2005

CPPS

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The naturally occurring polyamines, spermine [NH2(CH2)3NH(CH2)4NH(CH2)3NH2] and spermidine [NH2(CH2)3NH(CH2)4NH2], as well as the diamine putrescine [NH2(CH2)4NH2], are widely spread in nature. They occur in plants, micro-organisms and animal tissues and fulfil many important physiological functions. Due to their cationic nature they interact with negatively charged macromolecules such nucleic acids, phospholipids and proteins. This ionic interaction, which is reversible, leads to the stabilization of DNA, tRNA, membranes and some proteins. Early studies demonstrated that polyamines stimulate the growth of pro- and eukaryotic cells and that they play an important role in carcinogenesis and in malignant transformation processes. As a result of these studies various inhibitors of polyamine biosynthesis have been synthesized and are used to combat cancer and parasitic diseases (e.g., African sleeping sickness).

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“…were added. This procedure was adapted from Bretthauer et al (1963). Freshly harvested (zero time) spores were ground with a pre-chilled pestle for I min., spores harvested after incubation were ground 45 sec.…”

Section: Macromolecules and Germination In Fusarium 47mentioning

confidence: 99%

Synthesis of Macromolecules and Polyribosome Formation in Early Stages of Spore Germination in Fusarium solani

Cochrane

Rado

Cochrane

1971

Journal of General Microbiology

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SUMMARYNet synthesis of protein and RNA in germinating macroconidia of Fusarium solani began at the time of appearance of germ tubes, RNA being formed first; net DNA synthesis began much later. Studies on precursor incorporation indicated that the ungerminated spore, at the time of its removal from the parent mycelium, had a low but real capacity to synthesize both RNA and protein. Leucine incorporation rose very rapidly to a maximum at about 40 min. after harvest; even in the time required for conventional washing and filtration, incorporation capacity increased threefold. Ultracentrifuge profiles showed polysome peaks after I 5 min. incubation ; evidence from ribonuclease treatment was consistent with the existence of polysomes in the spore at the time of removal from the mycelium. Neither protein nor RNA synthesis required a complete medium, but DNA was synthesized only in a medium that supported germination.

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Amino Acid Incorporation into Protein by Cell-free Extracts of Yeast^*

Cited by 97 publications

References 6 publications

The Effect of Chloramphenicol on Growth and Mitochondrial Structure of Pythium ultimum

The Effect of Chloramphenicol on Growth and Mitochondrial Structure of Pythium ultimum

Naturally Occurring Polyamines: Interaction with Macromolecules

Synthesis of Macromolecules and Polyribosome Formation in Early Stages of Spore Germination in Fusarium solani

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Amino Acid Incorporation into Protein by Cell-free Extracts of Yeast*

Cited by 97 publications

References 6 publications

The Effect of Chloramphenicol on Growth and Mitochondrial Structure of Pythium ultimum

The Effect of Chloramphenicol on Growth and Mitochondrial Structure of Pythium ultimum

Naturally Occurring Polyamines: Interaction with Macromolecules

Synthesis of Macromolecules and Polyribosome Formation in Early Stages of Spore Germination in Fusarium solani

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Product

Resources

About

Amino Acid Incorporation into Protein by Cell-free Extracts of Yeast^*