Amino Acid Incorporation by a Bacterial Cell-Free System

Yoshida, Etsuko; Mitsui, Hiromi; Takahashi, Hiroki; Maruo, Bunji

doi:10.1093/oxfordjournals.jbchem.a127166

Cited by 8 publications

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“…Based on data obtained from the HMBC, 1 H-1 H COSY, and NOESY data (l " Fig. 2 and 3), the structure of 3 was assigned as the 2,25-dimethoxylated analogue of 5 and subsequently named scotanamine C. HCA amides have been found in many higher plants and exhibit a broad range of structure variations from simple phenolic amides [26,27] to complex macrocyclic polyamine alkaloids [28,29]. Plants belonging to the Solanaceae family, including Lycopersicon esculentum, Petunia hybrid, and Lycium chinense [30,31], are rich in HCA amide-containing species.…”

Section: Fliprmentioning

confidence: 99%

Amide Alkaloids from Scopolia tangutica

Zhang

Guo

et al. 2014

Planta Med

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Four new hydroxycinnamic acid amides, scotanamines A?D (1?4), and seven known alkaloids, including N 1,N 10-di-dihydrocaffeoylspermidine (5), scopolamine (6), anisodamine (7), hyoscyamine (8), anisodine (9), caffeoylputrescine (10), and N 1-caffeoyl-N 3-dihydrocaffeoylspermidine (11), were obtained from the roots of Scopolia tangutica. The present study represents the first recognition of hydroxycinnamic acid amides containing putrescine or spermidine in S. tangutica. Compound 1, in particular, contains a moiety resulting from the condensation of nortropinone and putrescine. Compound 2 exhibited moderate agonist activity at the ?-opioid receptor (EC50?=?7.3??M). Compound 2 was tested in vivo and induced analgesia in mice. The analgesic effect was recorded using the tail-flick assay and was reversed by naloxone.

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Section: Fliprmentioning

confidence: 99%

Amide Alkaloids from Scopolia tangutica

Zhang

Guo

et al. 2014

Planta Med

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“…Details of experiments in Pseudomonal-P described in this article have been and to be published in J. Biochem. (Tokyo) (25)(26)(27)(28)(29)(30).…”

Section: Amino Acid Incorporations Into Specific Protein In Vitromentioning

confidence: 99%

Protein Synthesis in Pseuomonas-P

Mitsui

1961

J. Gen. Appl. Microbiol.

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“…We obtained a subcellular preparation derived from spheroplasts from a strain of Pseudomonas, later classified as Pseudomonas schuylkilliensis (23). This preparation was able to oxidize various substrates, carry out oxidative phosphorylation, and synthesize protein and nucleic acids (28,41). The synthesis of protein and nucleic acids in these preparations was found to be dependent on oxidative phosphorylation (28,41).…”

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“…This preparation was able to oxidize various substrates, carry out oxidative phosphorylation, and synthesize protein and nucleic acids (28,41). The synthesis of protein and nucleic acids in these preparations was found to be dependent on oxidative phosphorylation (28,41). We are now interested in analyzing the relationship between the production of energy and its utilization for the synthesis of macromolecules.…”

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Synthesis of Protein and Nucleic Acid by Disrupted Spheroplasts of Pseudomonas schuylkilliensis

et al. 1971

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Osmotically shocked spheroplasts obtained from Pseudomonas schuylkilliensis strain P contained about 54,32,28, and 82% of the total cellular protein, ribonucleic acid (RNA), deoxyribonucleic acid (DNA), and phospholipid, respectively. This preparation was capable of incorporating 3apP-orthophosphate into RNA and DNA, aH-adenosine or 3H-uridine into RNA, and 3H-leucine or 14C-phenylalanine into protein. These activities were not found in the cytoplasmic fraction which contained most of the glucose-6-phosphate dehydrogenase activity. The synthesis of RNA by intact and disrupted spheroplast preparations was sensitive to actinomycin D, chromomycin Aa, streptovaricin, rifampin, Lubrol W, Triton X-100, and sodium deoxycholate, whereas RNA synthesis by intact cells was insensitive to these agents. Ethylenediaminetetraacetic acid, porcine pancreatic lipase, the protoplast-bursting factor, high concentrations of salts, and washing the preparation inhibited the synthesis of RNA by disrupted spheroplasts but had little or no effect on intact spheroplasts. Most of the newly synthesized RNA made by disrupted spheroplasts had the characteristics of messenger RNA. The DNA present in this preparation functioned as a template for RNA synthesis; continued protein synthesis was dependent on concomitant RNA synthesis. An unusual feature of the preparation was the finding that the synthesis of macromolecules was completely dependent on oxidative phosphorylation.

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Amino Acid Incorporation by a Bacterial Cell-Free System

Cited by 8 publications

References 0 publications

Amide Alkaloids from Scopolia tangutica

Amide Alkaloids from Scopolia tangutica

Protein Synthesis in Pseuomonas-P

Synthesis of Protein and Nucleic Acid by Disrupted Spheroplasts of Pseudomonas schuylkilliensis

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