Amino acid digestibility of corn distillers’ dried grains with solubles with the addition of casein in pigs1

Abstract

This experiment was conducted to determine the ileal digestibility of CP and AA in casein by regression analysis and to investigate the effects of casein in experimental diets on the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of CP and AA in corn distillers' dried grains with solubles (DDGS) fed to pigs. Twenty barrows (initial BW = 50.5 ± 4.46 kg) surgically fitted with T-cannulas at the distal ileum were used. Eighteen pigs were assigned to a triplicate 6 × 3 incomplete Latin Square design with 6 diets and 3 periods. Two pigs were used as replacements. Three diets were prepared to contain 60, 100, or 140 g/kg casein to determine the ileal digestibility of CP and AA in casein by regression analysis, and 2 diets were prepared to contain either 480 g/kg DDGS or 308 g/kg DDGS and 60 g/kg casein. Casein and DDGS were the sources of nitrogen (N) in experimental diets. A N-free diet was prepared based on cornstarch and dextrose. Experimental periods consisted of 5 d of adaptation and 2 d of ileal digesta collection periods. The AID of CP and AA in casein linearly increased (P < 0.01) with increasing concentration of casein in the diets. The SID of indispensable AA in casein ranged from 94.7% (SEM = 1.16) for Ile in pigs fed the diet containing 60 g/kg casein to 103.3% (SEM = 2.21) for Arg in pigs fed the diet containing 100 g/kg casein. Except for Arg, pigs fed the diet containing DDGS and casein had greater (P < 0.001) SID of CP and indispensable AA than those fed the diet containing DDGS without casein. The ileal digestibility of indispensable AA in casein determined by regression analysis ranged from 96.8% (SE = 3.14) for Thr to 103.1% (SE = 4.40) for Arg. The AID and SID of CP and AA for DDGS in the diet containing casein were calculated by difference method using the ileal digestibility of CP and AA in casein determined by regression analysis. The AID of Lys for DDGS in the diet containing casein was greater (P = 0.035) than those without casein; however, the AID of CP and the other indispensable AA, except for Arg, Phe, and Trp, for DDGS in the diet containing casein was less (P < 0.05) than those without casein. The SID of Lys and Phe for DDGS in the diet containing casein was greater (P < 0.05) than those without casein. In conclusion, improved AA composition in semi-purified experimental diets may affect the SID of AA in low-quality protein ingredients.

Digestibility of Amino Acids in Protein-Rich Feed Ingredients Originating from Animals, Peanut Flour, and Full-Fat Soybeans Fed to Pigs

“…We also investigated the digestibility of AA in casein, which is produced from pasteurized skim milk and is highly digestible [3,27]. As observed in this study, the SID of AA in casein agrees with previous reports [3,10,18] and was greater than the AA digestibility of EA, BM, or BPM.…”

“…However, it is pertinent to state that in contrast to the current study, previous studies [16,17] used spray dried egg by-products, not exclusively from EA, which may have caused the variations observed among studies. Casein was analyzed to contain similar or slightly greater gross energy and nutrients than as provided in NRC [10], but the analyzed gross energy and nutrient concentrations were close to the values reported in another study [18]. The concentrations of gross energy and nutrients in BM and BPM were similar to the NRC [10].…”

Amino acid supplementation during the adaptation period did not affect the standardized ileal digestibility of amino acids in corn and soybean meal fed to pigs

“…The improved AID and SID of CP and Pro in the corn with AA supplementation at the adaptation period, but without increased AID and SID of CP and Pro in SBM were observed in this study. The reason for the only increase of AID and SID of CP and Pro in corn may be due to less Pro concentrations in ileal digesta in corn-based diet with AA supplementation group that are agreement with pigs fed corn distillers dried grains with solubles with casein diets [ 5 ]. In pigs fed an N-free diet, the high protein mobilization resulted in relatively greater dispensable AA especially in Pro and Gly contents in ileal digesta [ 19 ].…”

“…To determine ileal AA digestibility in a feed ingredient of interest, the ingredient is included in the experimental diet as the sole source of AA. Thus, AA compositions in experimental diets are often insufficient or imbalanced for maintaining a normal physiological condition of pigs, particularly in an N-free diet and cereal grain-based diets [ 5 ].…”

Influence of Distillers Dried Grains With Solubles on Quail Meat Productivity

“…Distillers dried grain with solubles (thereinafter -bard) is also widely used in diets for pigs, as it contains a sufficient amount of proteins (Park et al, 2018;Rho et al, 2018).…”