Amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro

Koch, Franziska; Wolff, Anne; Mathes, Stephanie; Pieles, Uwe; Saxer, Sina; Kreikemeyer, Bernd; Peters, Kirsten

doi:10.2147/ijn.s173702

Cited by 13 publications

(17 citation statements)

References 44 publications

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“… Koch et al (2018) produced the 11-amino acid self-assembling peptides P11-8 and P11-28/29 hydrogels exhibiting ζ-potentials of ∼ + 20 mV and ∼ + 60 mV at pH 7.0, respectively. HPDLF and HCO cells on highly positive P11-28/29 surfaces showed reduced cell area after 24 h, while cells on P11-8 displayed also enhanced osteogenic differentiation (ALP activity) compared with hydrogels containing negatively charged glutamic acids.…”

Section: Discussionmentioning

confidence: 99%

“…On PPI-G4 and PDADMA surfaces with highly positive ζ-potentials we discovered a restricted cell viability, reflected in an impaired Ca 2+ mobilization and a disturbed cell morphology and proliferation after 24 h, indicating that cells cannot tolerate such ζ-potentials. Koch et al (2018) produced the 11-amino acid self-assembling peptides P11-8 and P11-28/29 hydrogels exhibiting ζ-potentials of ∼ + 20 mV and ∼ + 60 mV at pH 7.0, respectively. HPDLF and HCO cells on highly positive P11-28/29 surfaces showed reduced cell area after 24 h, while cells on P11-8 displayed also enhanced osteogenic differentiation (ALP activity) compared with hydrogels containing negatively charged glutamic acids.…”

Section: Moderately Positive ζ-Potential (+1 To +10 Mv)mentioning

confidence: 99%

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Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Gruening

Neuber

Nestler

et al. 2020

Front. Bioeng. Biotechnol.

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Electrostatic forces at the cell interface affect the nature of cell adhesion and function; but there is still limited knowledge about the impact of positive or negative surface charges on cell-material interactions in regenerative medicine. Titanium surfaces with a variety of zeta potentials between −90 mV and +50 mV were generated by functionalizing them with amino polymers, extracellular matrix proteins/peptide motifs and polyelectrolyte multilayers. A significant enhancement of intracellular calcium mobilization was achieved on surfaces with a moderately positive (+1 to +10 mV) compared with a negative zeta potential (−90 to −3 mV). Dramatic losses of cell activity (membrane integrity, viability, proliferation, calcium mobilization) were observed on surfaces with a highly positive zeta potential (+50 mV). This systematic study indicates that cells do not prefer positive charges in general, merely moderately positive ones. The cell behavior of MG-63s could be correlated with the materials' zeta potential; but not with water contact angle or surface free energy. Our findings present new insights and provide an essential knowledge for future applications in dental and orthopedic surgery.

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Section: Discussionmentioning

confidence: 99%

Section: Moderately Positive ζ-Potential (+1 To +10 Mv)mentioning

confidence: 99%

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Gruening

Neuber

Nestler

et al. 2020

Front. Bioeng. Biotechnol.

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“…Another valuable use of the terahertz FEL is the application as fabrication tool for biomaterial. Peptide fibril can be used as fiber-type scaffold for biocompatible materials such as cell sheets in regenerative medicine and nano-carrier in drug-delivery system 30,31 . Intense terahertz laser would be expected to become a novel processing tool of those biomaterials both in vivo and in vitro .…”

Section: Discussionmentioning

confidence: 99%

Dissolution of a fibrous peptide by terahertz free electron laser

Kawasaki

Tsukiyama

Irizawa

2019

Sci Rep

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Fibrous peptides such as amyloid fibrils have various roles in biological system, e.g., as causal factor of serious amyloidosis in human and as functional regulator of cell formation in bacteria and eukaryotes. In addition, the fiber-type format is promising as biocompatible scaffold. Therefore, the dissolution method of peptide fibril is potentially useful at many scenes in medical and material fields: as reductive way of pathogenic amyloid, as modification technique of cell structure, and as fabrication tool of biomaterials. However, the fibril structure is generally difficult to be dissociated due to its rigid stacked conformation. Here, we propose a physical engineering technology using terahertz free electron laser (FEL) at far-infrared wavelengths from 70 to 80 μm. Infrared microscopy analysis of the irradiated fibril of calcitonin peptide as a model showed that β-sheet was decreased, and α-helix, turn, and others were increased, compared to those of the fibril before the FEL irradiation. Interestingly, the dissociative effect by the far-infrared laser was remarkable than that by the mid-infrared laser tuned to 6.1 μm that corresponds to amide I. In addition, simple heating at 363 K deformed the fibril state but increased the amount of β-sheet, which was contrast with the action by the FEL, and scanning-electron microscopy and Congo-red staining revealed that the fibril was collapsed power-dependently within a range from 25 to 900 mJ energies supplied with the FEL at 74 μm. It can be considered that irradiation of intense terahertz wave can dissociate fibrous conformation of peptide with little influence of thermal effect.

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“…15A-C) -can be exploited to promote cell adhesion, migration, proliferation and differentiation and thereby eventually support restoration of damaged soft tissue. [38][39][40] Human Periodontal Ligament Fibroblasts (HPDLF) expressed a stretched cell phenotype with actin stress fiber formation, after 24 h of culture on P11 hydrogels (Fig. 15D).…”

Section: Biomaterials For Dental Therapymentioning

confidence: 99%

“…Noticeably, P11-4 hydrogels strengthen the osteogenic differentiation of DFSCs compared with TCPS. [39] 5.2 Nano-structured Mixed Mode Metal Catalyst Immobilized on Self-supporting Carrier…”

Section: Biomaterials For Dental Therapymentioning

confidence: 99%