1979
DOI: 10.1016/s0003-2697(79)80133-5
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Amino acid analysis with o-phthalaldehyde detection: Effects of reaction temperature and thiol on fluorescence yields

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Cited by 64 publications
(25 citation statements)
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“…When the presence of salts resulted in ambiguous identification of amino acids, we did not report the results in the tables. Kvenvolden et al 1970;Pereira et al 1975;Cronin et al 1979a;Cronin and Pizzarello 1983;Peltzer et al 1984;Engel et al 1990;Engel and Macko 1997. b nmol/g = nanomol of amino acid per gram of meteorite. c n.d. = not determined.…”
Section: Resultsmentioning
confidence: 99%
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“…When the presence of salts resulted in ambiguous identification of amino acids, we did not report the results in the tables. Kvenvolden et al 1970;Pereira et al 1975;Cronin et al 1979a;Cronin and Pizzarello 1983;Peltzer et al 1984;Engel et al 1990;Engel and Macko 1997. b nmol/g = nanomol of amino acid per gram of meteorite. c n.d. = not determined.…”
Section: Resultsmentioning
confidence: 99%
“…AIB and isovaline, both α-dialkyl amino acids, were identified by a two-step analysis, where the post column OPA-reaction loop was kept at 25 °C in one analysis and at 100 °C in the other. Since the α-dialkyl amino acids react more slowly with the fluorescent reagent (Cronin et al 1979a), higher temperature derivatization reactions produce a marked increase in peak height, which is specific to these amino acids. This two-temperature method has frequently been used to unequivocally identify meteoritic amino acids (Cronin et al 1979a(Cronin et al , 1979bCronin and Pizzarello 1983).…”
Section: Amino Acid Analysis Methodsmentioning
confidence: 99%
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“…The solution was then added to 1,OOO ml of 0.4M potassium borate buffer, pH 10, that had been previously filtered through a membrane with 0.22-pn pore size, then deoxygenated by bubbling with chromatography-grade nitrogen through a fritted disk for 10 minutes. The OPA reagent was protected from light and oxygen at all times (19) and stored at 4°C until use. During post-column derivatization, the OPA reagent was delivered at room temperature to the mixing tee at a rate of 0.25 muminute, using a Model A-30-S Eldex single piston pump (Eldex Laboratories, Menlo Park, CA) with stroke length adjusted to 0.060".…”
Section: Methodsmentioning
confidence: 99%
“…In those applications, the reagent includes OPA and a thiol such as methanethiol, ethanethiol, or mercaptoethanol, with the amines as the limiting reagent (Cronin et al, 1979;Wong el al., 1985;Rapsomanikis et al, 1988, and references therein). An OPA-thiol-amine reaction has been used to analyze a variety of thiols in natural waters and biological samples, by providing excess OPA and ethanolamine and allowing the thiol to be limiting (Nakamura and Tamura, 1981;Mopper and Delmas, 1984).…”
Section: Principles Of Operationmentioning
confidence: 99%