Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory

Macchi, Frank; Shen, Felicity J.; Keck, Rodney G.; Harris, Reed J.

doi:10.1385/1-59259-047-0:009

Cited by 26 publications

(21 citation statements)

References 23 publications

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“…A factor of 6.25 was used to convert N to protein. The amino acid content was determined in duplicate based on a method adapted from Ozols (), and Macchi, Shen, Keck and Harris (). Bean flour samples (10 mg) were mixed with 200 µl 6N HCl/1% phenol and hydrolyzed at 110 °C for 24 hr to release amino acids.…”

Section: Methodsmentioning

confidence: 99%

Characterization of pinto bean high-starch fraction after air classification and extrusion

Simons

Hall²,

Biswas

2017

J Food Process Preserv

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The properties of three bean flours (whole, high‐starch fraction, and extruded) were studied to determine their potential applications. Significant differences in moisture, protein, resistant starch, total starch, lipids, ash, phytic acid, amino acid content, and fatty acid profile were observed among the three flours, i.e., whole pinto flour (WPF), non‐extruded high starch fraction (NE‐HSF), and extruded high starch fraction (E‐HSF). E‐HSF had the lowest L* value, indicating a darkening of pinto bean flour due to extrusion. Extrusion significantly increased the water solubility index (WSI) and water absorption index (WAI). Thermal properties of NE‐HSF indicated that it underwent lower transition temperatures than WPF. Air‐classification produced NE‐HSF with a significantly higher pasting viscosity compared to WPF. However, extrusion reduced pasting viscosity. The differences in the bean flour properties provide insights into potential for new product formulations from bean high starch fractions that will meet unique functional and nutritional needs. Practical applications Availability of value‐added ingredients from dry edible beans is limited. This research explored properties of the air‐classified high‐starch fraction of pinto beans and its extrudates. The purpose was to determine ways in which the high starch fraction and its extrudates can be utilized. This will help to expand bean ingredient options beyond the use of whole edible bean flours. Furthermore, value‐added ingredients from dry beans will provide suitable alternatives to soybean which is a known allergen. Other concerns with soybean are, the use of genetically modified organisms (GMO) in its production, and concerns about the effect of soybean consumption on estrogen activity in the body.

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Section: Methodsmentioning

confidence: 99%

Characterization of pinto bean high-starch fraction after air classification and extrusion

Simons

Hall²,

Biswas

2017

J Food Process Preserv

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“…Quantitative analysis of amino acids in plasma was determined by ion-exchange HPLC with post-column derivatization using ninhydrin. The amino acids were detected by UV-vis at 570 nm and data integration was performed with PeakNet software version 6.30 (Dionex, Sunnyvale, CA, USA) (Macchi et al 2000).…”

Section: Methodsmentioning

confidence: 99%

Anaplerotic diet therapy in inherited metabolic disease: Therapeutic potential

Roe

Mochel

2005

J of Inher Metab Disea

117

131

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Beginning with phenylketonuria, dietary therapy for inborn errors has focused primarily on the restriction of the precursor to an affected catabolic pathway in an attempt to limit the production of potential toxins. Anaplerotic therapy is based on the concept that there may exist an energy deficit in these diseases that might be improved by providing alternative substrate for both the citric acid cycle (CAC) and the electron transport chain for enhanced ATP production. This article focuses on this basic problem, as it may relate to most catabolic disorders, and provides our current experience involving inherited diseases of mitochondrial fat oxidation, glycogen storage, and pyruvate metabolism using the anaplerotic compound triheptanoin. The observations have led to a realization that 'inter-organ' signalling and 'nutrient sensors' such as adenylate monophosphate mediated-protein kinase (AMPK) and mTOR (mammalian target of rapamycin) appear to play a significant role in the intermediary metabolism of these diseases. Activated AMPK turns on catabolic pathways to augment ATP production while turning off synthetic pathways that consume ATP. Information is provided regarding the inter-organ requirements for more normal metabolic function during crisis and how anaplerotic therapy using triheptanoin, as a direct source of substrate to the CAC for energy production, appears to be a more successful approach to an improved quality of life for these patients.

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“…Separation and determination of amino acids are currently performed mainly by cation-exchange chromatography with post-column derivatization and spectrophotometric detection [8][9][10], reversedphase high-performance liquid chromatography with pre-column derivatization and spectrophotometric detection [11 13], gas chromatography with derivatization [14], and capillary electrophoresis [15,16]. Separations and determinations of carbohydrates are performed mainly by gas chromatography with derivatization [17], high-performance liquid chromatography with refractive index detection or derivative spectrophotometric detection [18], high-performance anion-exchange chromatography with pulsed amperometric detection [19,20], and capillary electrophoresis [21,22].…”

Section: Introductionmentioning

confidence: 99%

Direct and simultaneous determination of amino acids and sugars in rice wine by high-performance anion-exchange chromatography with integrated pulsed amperometric detection

Ding

Mou

2003

Chromatographia

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KeyWordsColumn liquid chromatography Anion exchange chromatography Integrated pulsed amperometry Amino acids and sugars Rico wne SummaryA method for dTrect simuhaneous determTnation of amTno acids and sugars, including monosaccharides, disaccharides, trisaccharides, and tetrasaccharTdes, Tn rice wine by high-performanco anion-exchange chromatography wth integrated pulsed amperometrTc detection is described.The mobile phase gradientwas prepared from aqueous solutions of sodium hydro xide and sodium acetate. The effects of mobile phase composition and column temperature on the separation of the amino adds and sugars were investigated in detail and optimized conditions were determined for analysis of amino acids and sugars in rice wne. The time ta ken to run the gradient was 90 rain. The column temperature was 30 ~ Amino acids and su gars in the rice wnes were identified by using ~o different waveforms and comparing the re suhs from each. Under the optTmTzed conditTons detectTon ITmTts for the amino acids and sugars were O. 11 -12.80 pmol. RSD of peak areas were 0.3 -3.7% (n -5). Calibration graphs of peak area for the analyies were ITnear over approximately three orders of magnTtude. Ten amTno acids and eTght sugars Tn rico wine were Tdentified and quantified. Recoveries of these components afier spiking were 95.4-105.8%. Chromatograms obtaTned from rice wne underoptTmTzed gradient conditTons were used for rough assessment of the quality of the wine.

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Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory

Cited by 26 publications

References 23 publications

Characterization of pinto bean high-starch fraction after air classification and extrusion

Characterization of pinto bean high-starch fraction after air classification and extrusion

Anaplerotic diet therapy in inherited metabolic disease: Therapeutic potential

Direct and simultaneous determination of amino acids and sugars in rice wine by high-performance anion-exchange chromatography with integrated pulsed amperometric detection

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