Amino Acid Analysis of Peptides Using HPLC With Evaporative Light Scattering Detection

Peterson, Jeffery A.; Lorenz, Leslie J.; Risley, Donald S.; Sandmann, Beverly J.

doi:10.1081/jlc-100101714

Cited by 25 publications

(9 citation statements)

References 43 publications

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“…Recently, some excellent work has been carried out by Peterson et al [19], who have used E L S D to detect underivatised amino acids at high sensitivity. The same amino acids have been used for a comparison with the current detector (Table II).…”

Section: Comparison Of the Unimas Tm 201 With Evaporative Light Scamentioning

confidence: 98%

The UnimasTM detector: A near-universal interface between HPLC and GC

Scott¹,

Little²,

Peña³

2001

Chromatographia

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SummaryA device is described wherein the solvent is removed from the liquid chromatographic eJuent, prior to immobilising the solute onto a moving, oxidised, titanium tape. The latter is wetted by all solvents, including water. The tape then moves the solute remaining into a pyrolysis zone, integral with a GC detector. For high sensitivily universal operation, a modified Argon Ionisation detector has been constructed. Gaseous pyrolysis products are swept into the detector. An alternative design has also been developed comprising a dual Argon Ionisation / Electron capture detector for high sensitivily in both universal and selective modes.

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Section: Comparison Of the Unimas Tm 201 With Evaporative Light Scamentioning

confidence: 98%

The UnimasTM detector: A near-universal interface between HPLC and GC

Scott¹,

Little²,

Peña³

2001

Chromatographia

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“…Two substance mixtures consisting of 24 pesticides and 18 amino acids are used for the characterization of the IM detector. The following compounds are contained in the pesticide mixture (Supporting Information: Table S1): diquatdibromide(1), aldicarbsulfoxide(2), imidacloprid(3), aldicarbsulfone(4), methomyl(5), oxamyl(6), chloridazone (7), metoxurone(8), 3-hydroxycarbofurane (9), aldicarb(10), monurone (11), propoxur (12), diurone (13), chlortolurone (14), carbofuran (15), carbaryl (16), methabenzthiazurone(17), monolinurone (18), isoproturone (19), metobromurone (20), metazachlor (21), methiocarb (22), linurone(23) and metolachlor (24). The amino acid mixture is comprised of the following compounds: DL-methionine (Met), DL-valine (Val), L-glutamine (Gln), glutamic acid (Glu), glycine (Gly), L-histidine (His), L-alanine (Ala), L-arginine (Arg), L-aspartic acid (Asp), L-asparagine (Asn), L-cysteine (Cys), L-isoleucine (Ile), L-leucine (Leu), L-phenylalanine (Phe), L-proline (Pro), L-serine (Ser), L-threonine (Thr), L-tyrosine (Tyr) and L-tryptophan (Trp).…”

Section: Chemicalsmentioning

confidence: 99%

High‐performance liquid chromatography with electrospray ionization ion mobility spectrometry: Characterization, data management, and applications

Zühlke

Riebe

Beitz

et al. 2016

J of Separation Science

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The combination of high-performance liquid chromatography and electrospray ionization ion mobility spectrometry facilitates the two-dimensional separation of complex mixtures in the retention and drift time plane. The ion mobility spectrometer presented here was optimized for flow rates customarily used in high-performance liquid chromatography between 100 and 1500 μL/min. The characterization of the system with respect to such parameters as the peak capacity of each time dimension and of the 2D spectrum was carried out based on a separation of a pesticide mixture containing 24 substances. While the total ion current chromatogram is coarsely resolved, exhibiting coelutions for a number of compounds, all substances can be separately detected in the 2D plane due to the orthogonality of the separations in retention and drift dimensions. Another major advantage of the ion mobility detector is the identification of substances based on their characteristic mobilities. Electrospray ionization allows the detection of substances lacking a chromophore. As an example, the separation of a mixture of 18 amino acids is presented. A software built upon the free mass spectrometry package OpenMS was developed for processing the extensive 2D data. The different processing steps are implemented as separate modules which can be arranged in a graphic workflow facilitating automated processing of data.

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“…The concept and operation of commercially available ELSDs as sensitive universal detectors has been previously discussed in the literature [17]. The ELSD has been shown to successfully detect many substances such as phospholipids [18 -21], triglycerides, fats and fatty acid esters [22,23], carbohydrates [24 -26], synthetic polymers [27], steroids [28], and amino acids [29,30]. The HPLC-ELSD system has also been extremely useful for the determination of pharmaceutical impurities, raw materials, cleaning verification, and small organic compounds [31 -34].…”

Section: Introductionmentioning

confidence: 99%

Analysis of mannitol in pharmaceutical formulations using hydrophilic interaction liquid chromatography with evaporative light-scattering detection

Risley¹,

Yang

Peterson

2006

J. Sep. Science

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This study demonstrates the use of hydrophilic interaction liquid chromatography (HILIC) for the separation of both active and inactive ingredients in pharmaceuticals from a single injection. Excipients commonly used in parenteral formulations were separated using a gradient method employing increasing aqueous composition. An evaporative light-scattering detector (ELSD) provided direct detection of inactive excipients and inorganic salts lacking UV chromophores. Analyses of Gemzar parenteral formulations using optimized isocratic HILIC-ELSD method conditions were performed based on retention time screening from the gradient assay. All of the components were efficiently separated using a TSK-Gel Amide 80 column including gemcitabine, mannitol, and sodium cation demonstrating the qualitative capability of the technique. The method was thoroughly validated for mannitol content to access the quantitative potential of the technique. Validation parameters included linearity, accuracy, specificity, solution stability, repeatability, and intermediate precision. Overall, the method described in this report proved to be very robust and represents a novel technique to conveniently separate and detect the active and inactive components in pharmaceuticals both quickly and accurately.

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Amino Acid Analysis of Peptides Using HPLC With Evaporative Light Scattering Detection

Cited by 25 publications

References 43 publications

The UnimasTM detector: A near-universal interface between HPLC and GC

The UnimasTM detector: A near-universal interface between HPLC and GC

High‐performance liquid chromatography with electrospray ionization ion mobility spectrometry: Characterization, data management, and applications

Analysis of mannitol in pharmaceutical formulations using hydrophilic interaction liquid chromatography with evaporative light-scattering detection

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