Amine-modified hyperbranched polyesters as non-toxic, biodegradable gene delivery systems

Hyperbranched Polyamines for Transfection

“…It has been reported that biodegradable cationic polymers are nontoxic and condense DNA into compact particles that can transfect mammalian cells. Furthermore, the degradation of drug carriers can also be used to control the release of the DNA inside the cell [109][110][111][112]. Pack et al developed a flexible, biodegradable, hyperbranched system based on PEI [113].…”

“…The degradation of gene delivery polymers in vivo is of high significance for efficient therapeutic delivery because the appropriate degradation of the polymer into low molecular weight breakdown products enables the reduction of cytotoxicity by reducing cumulative cellular exposure time and an easy elimination by excretion pathways in vivo [23,109,110]. It has been reported that biodegradable cationic polymers are nontoxic and condense DNA into compact particles that can transfect mammalian cells.…”

“…Kissel et al investigated amine-modified hyperbranched polyesters by introducing tertiary amines on polymers based on 2,2-bis-(methylol)propionic acid (bis-MPA) (Boltorn H 1 , a commercially available hyperbranched polymer (HBP)) [110]. By using carbonyldiimidazole chemistry the terminal hydroxyl (-OH) groups from Boltorn were esterified with increasing equivalents (9,19,35,48, and 60) of diethylaminopropylamine (DEAPA) (Scheme 6).…”

Disulfide‐Containing Hyperbranched Polyethylenimine Derivatives via Click Chemistry for Nonviral Gene Delivery

“…[27][28][29] Kissel and coworkers [30] described amine-modified hyperbranched polyesters as non-toxic, Reducible disulfide-containing hyperbranched PEI-SS-HP was synthesized via click chemistry and evaluated as nonviral gene carrier. The structure of the polymers was confirmed by 1 H NMR and FTIR.…”

siRNA transfection by dendritic core–shell nanocarriers

“…[298] Many research groups generate biodegradable polycations, e.g., by cross-linking nontoxic low molecular weight monomers or oligomers with linkers containing labile bonds. Green et al [117,300] reported, e.g., poly(β-amino ester)s as promising materials because they bind and self-assemble with DNA to form stable nanoparticles that effectively enter cells, escape the endosomal compartments, and degrade via hydrolytic cleavage of backbone ester groups. [117] Another approach regarding pH-degradable dendritic architectures has been presented by Wu et al [301] They have set up an approach with linear poly(amino ester)s for DNA delivery based on the Michael addition polymerization of trifunctional amines and diacrylates which can release the DNA through hydrolyzable ester groups.…”