Aminco-Bowman Spectrophotofluorometer Modification-II

Abstract

We have modified a standard Aminco-Bowman spectrophotofluorometer to allow collection of spectral data on punched paper tape. Using this tape, we have obtained high quality corrected spectra by postprocessing techniques on a digital computer. We have examined the errors associated with changes in the energy distribution of the excitation source.

Correction of inner filter effects in fluorescence spectrometry

“…Slits were selected to give the maximum spectral resolution (~0.75 nm) consistent with maintaining at least a 15:1 signahnoise ratio. Standard techniques were used to obtain the spectral distribution of the excitation source (5,6).…”