Aminated Single-walled Carbon Nanotubes as Carrier for Covalent Immobilization of Phenylalanine Ammonia-lyase

Abstract

Abstract

“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media

“…The reusability of these biocatalysts were tested also under more demanding conditions in the opposite reaction direction to catalyze ammonia addition onto trans -cinnamic acid ( trans -CA) producing l -phenylalanine. The activity of the Pc PAL-MNPs-THPMTGE/EDa-10 was barely diminished during the recycling even of the negative effect of the long-term presence of high ammonia concentration [123]. Both biocatalysts produced l -phenylalanine at high conversion rate with an excellent enantiomeric excess even after five repeated batch reactions (Table 5).…”

“…Also the pH during the immobilization has a great impact on the formation of stabile multipoint covalent attachment between the enzyme and the support [114,120]. Nowadays, a great variety of supports are used: macroporous polymers [107,109], silica particles in different size and shape [21], magnetic nanoparticles [121] and several type of nanofibers [122] or nanotubes [123] are just some of the most popular ones. Also modification of the surface with inert groups besides the reactive functions has an effect on the biocatalytic activity [103,109].…”

Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens

“…The functionalization of carbon nanotubes was carried out as earlier described [46]. SwCNT (400 mg) and urea (400 mg) were suspended in dimethyl formamide (DMF, 5 mL).…”

Conservation of the Biocatalytic Activity of Whole Yeast Cells by Supported Sol – Gel Entrapment for Efficient Acyloin Condensation

“…Even today -due to the extremely expanding requirements of biotechnologyenzyme and cell immobilization is a rapidly developing area. The main aims of immobilization strategies are the isolation, compartmentalization and stabilization of the valuable cells, cell organelles or enzymes with high biocatalytic activity [14][15][16][17][18]. Although several immobilization methods and carrier systems for cells or enzymes are based on similar methods (binding reactions and media, carrier materials etc.…”

“…Among cell immobilization techniques, adsorption on tissue-like or macroporous solid supports and entrapment in gel matrices such as alginate, polyacrylamide, carrageenan, chitosan or agar based matrices are used commonly [18][19][20][21][22][23]. In most of these cases, traditional organic materials are applied which are natural, biocompatible, commercially available and easy-to-handle.…”