Amikacin Resistance in Staphylococcus pseudintermedius Isolated from Dogs

Gold, Randi M.; Cohen, Noah D.; Lawhon, Sara D.

doi:10.1128/jcm.01253-14

Cited by 22 publications

(19 citation statements)

References 22 publications

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“…The bifunctional acetyltransferase/phosphotransferase gene aac(6′)-Ie–aph(2′)-Ia was present only in one gentamicin resistant strain. Comparable results were found in a similar study performed in a veterinary medical teaching hospital in Texas, where the most common aminoglycoside resistance gene found was aph(3′)-IIIa , followed by aac(6′)/aph(2′′) and ant(4′)-Ia genes [20]. Most of the resistance genes detected in S. pseudintermedius have also been identified in other species of staphylococci.…”

Section: Discussionsupporting

confidence: 61%

Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina

et al. 2019

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Background Staphylococcus pseudintermedius is the leading cause of pyoderma in dogs and the frequent use of antimicrobial treatment is associated to the development of resistance to nearly all classes of antibiotics. Despite S. pseudintermedius significance, our understanding of the molecular mechanism of β-lactam resistance and its genetic diversity remains limited. We aimed to: i ) determine the phenotypic resistance profile of methicillin resistant Staphylococcus pseudintermedius (MRSP) isolated from infected dogs in three different veterinary hospitals in Buenos Aires, Argentina; ii ) identify the SCC mec elements and resistance genes; and iii ) analyze the clonal relationship between isolates and in regard of dominant lineages found in the world. Results In addition to the differential levels of β-lactam resistance, MRSP isolates ( n = 10) showed resistance to 5–6 families of antibiotics, and were therefore categorized as multidrug-resistant. All the isolates were variant of SCC mec V homologous to S. aureus ; additional SCC mec Finder analysis classified five of the genomes as SCC mec type V (5C2&5) with mecA (encodes for PBP2a) , mecRI and mecI and all the genes closely related to the reference SCC mec type V S. aureus TSGH17 strain. In the remaining five strains, mecA was present, although other genes associated with SCC mec V including mecR1 and mecI were missing. PBP2a was inducible in low level resistance strains (MRSP 8151), and constitutively expressed in MRSP 8150, suggesting different mecA regulatory mechanisms. MRSP isolates showed significant genetic diversity: eight PFGE clonal types and six multilocus-sequence typing (MLST) sequence types (STs) (339, 649, 919, 920, 921 and 922), including four new STs genetically distinct from STs reported in other geographic areas. Comparative genomics and phylogenetic analyses of the MRSP showed a correlation between the genetic content and the phenotypes, and established the genetic relationship between the isolates. Conclusions MRSP could be a threat to animal health due to it concerning level of antimicrobial resistance. Our study highlights genetic and epidemiological aspects of multidrug-resistant MRSP strains from Argentina showing high degree of correlation between the resistance genes and the phenotype of the isolates and, furthermore, they appeared evolutionary closer to major worldwide reported ST68 and...

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Section: Discussionsupporting

confidence: 61%

Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina

et al. 2019

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“…The high percentage of resistance of Staphylococcus species isolates to Trimethoprim-Sulfamethoxazole, Vancomycin, Doxycycline, Ciprofloxacin, Enrofloxacin, and Amoxicillin/Clavulanate agrees with previous studies reporting the resistance of Staphylococcus species to amikacin (36), lincosamides (37), clarithromycin (38), cephalexin (39), and quinolones (40,41). We also found a 36% resistance to Oxacillin, suggesting that these isolates probably have the mecA gene.…”

Section: Discussionsupporting

confidence: 91%

Molecular Detection and Characterization of the mecA and nuc Genes From Staphylococcus Species (S. aureus, S. pseudintermedius, and S. schleiferi) Isolated From Dogs Suffering Superficial Pyoderma and Their Antimicrobial Resistance Profiles

et al. 2020

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Canine superficial pyoderma (CSP) is a bacterial infection secondary to several skin diseases of the dog. Staphylococcus pseudintermedius, which is a commensal bacterium of the dog's skin, is the leading agent found in dogs affected by CSP, which can progress to deep pyoderma. It is also of clinical significance because S. pseudintermedius strains carry antimicrobial resistance genes, mainly the mecA gene. In this descriptive longitudinal study, molecular characterization of bacterial isolates from dogs affected by CSP was performed in addition to phenotyping, antimicrobial profiling, and assessment of resistance carriage status. Fifty dogs (24 females and 26 males) attending the CES University Veterinary Teaching Hospital were included in the study. CSP was confirmed according to clinical signs and cytological examination. Swabs were taken from active skin lesions for bacterial culture, and phenotyping and antimicrobial resistance profiles were assessed using API-Staph phenotyping and the Kirby-Bauer method, respectively. We also performed molecular detection and characterization of the mecA and nuc encoding gene of coagulase-positive Staphylococci. The mecA gene frequency was established by qPCR amplification of a 131bp gene fragment. Data were evaluated by descriptive statistics. Erythema, peeling, pruritus, and alopecia were the predominant symptoms (72, 56, and 46%, respectively). We isolated bacteria compatible with Staphylococcus species from all samples tested. González-Domínguez et al. mecA/nuc Genes From Staphylococcus Species API phenotyping showed 83.1 to 97.8% compatibility with S. pseudintermedius. PCR-genotyping resulted in 15, 3, and 1 isolates positive for S. pseudintermedius, S. aureus, and S. schleiferi, respectively. Isolated strains showed high susceptibility to Imipenem, Ampicillin/Sulbactam, and Rifampicin (100, 94, and 92%, respectively). The highest resistance was against Vancomycin and Trimethoprim/Sulfamethoxazole (98 and 74%, respectively). S. pseudintermedius, S. aureus, and S. schleiferi isolates were cloned and shared 96% sequence homology. Finally, we found 62% carriage status of the mecA gene in isolates of CSP patients, although only 36% of the isolates were methicillin-resistant. Identification of three Staphylococcus species causing CSP, high-level resistance against conventional antimicrobials, and carriage of the mecA gene highlight the importance of performing molecular characterization of bacteria causing dermatological conditions in dogs.

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“…MRSP isolates are commonly multi-drug resistant, but resistance to amikacin and rifampicin is an emerging trait. Amikacin-resistant strains have been isolated with increasing frequency from canine patients [ 26 ] but not from healthy dogs. In S. pseudintermedius , the most common amikacin resistance gene was found to be aph(3′)-IIIa [ 26 ], which is present in the isolates detected here.…”

Section: Discussionmentioning

confidence: 99%

“…Amikacin-resistant strains have been isolated with increasing frequency from canine patients [ 26 ] but not from healthy dogs. In S. pseudintermedius , the most common amikacin resistance gene was found to be aph(3′)-IIIa [ 26 ], which is present in the isolates detected here. Aminoglycosides such as amikacin are among the last choices of treatment for staphylococcal infections and have not been used on the bitches included in this study.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and characterization of methicillin-resistant Staphylococcus pseudintermedius in successive parturitions of bitches and their puppies in two kennels in Italy

et al. 2018

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BackgroundMulti-drug methicillin-resistant Staphylococcus pseudintermedius (MRSP) detection is rapidly increasing in microbial specimens from pets across Europe. MRSP has also been isolated from bitches and newborns in dog breeding kennels. This study assessed whether MRSP lineage differs between breeding kennels and is maintained over time. Post-partum bitches (at day 3 vaginal and day 3, 9 and 35 milk samples) and their litters (at day 3, 9 and 35 oral and abdominal skin samples) from two Italian breeding kennels (A and B) were sampled and MRSP was subsequently characterized via whole-genome sequencing and antibiotic susceptibility testing. The study was carried out from October 2014 to March 2016 and included successive parturitions from the same animals.ResultsThe analysis revealed different situations in both investigated kennels. In kennel A, circulating strains were from 7-locus sequence types ST688, ST258 and closely related isolates of ST71, which included most isolates. In kennel B, only a new isolate, ST772, was detected. In addition, most isolates from both kennels had multi-resistant antibiotic profiles. MRSP was only isolated from litters of MRSP-positive bitches, thus suggesting that bitch-litter transmission is likely.ConclusionsOur data show that MRSP circulation can differ in different settings, that several clonal lineages can circulate together, and that vertical transmission appears common. MRSP colonization did not affect the health conditions of the bitches or of their litters.Electronic supplementary materialThe online version of this article (10.1186/s12917-018-1612-z) contains supplementary material, which is available to authorized users.

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Amikacin Resistance in Staphylococcus pseudintermedius Isolated from Dogs

Cited by 22 publications

References 22 publications

Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina

Identification and molecular epidemiology of methicillin resistant Staphylococcus pseudintermedius strains isolated from canine clinical samples in Argentina

Molecular Detection and Characterization of the mecA and nuc Genes From Staphylococcus Species (S. aureus, S. pseudintermedius, and S. schleiferi) Isolated From Dogs Suffering Superficial Pyoderma and Their Antimicrobial Resistance Profiles

Occurrence and characterization of methicillin-resistant Staphylococcus pseudintermedius in successive parturitions of bitches and their puppies in two kennels in Italy

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