Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm

Maszewska, Agnieszka; Moryl, Magdalena; Wu, Jianyu; Liu, Bin; Feng, Lu; Różalski, Antoni

doi:10.1038/s41598-020-80907-9

Cited by 8 publications

(5 citation statements)

References 51 publications

(39 reference statements)

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“…Struvite and apatite crystals are deposited in biofilms to form crystalline biofilms ( Armbruster et al, 2018 ). The presence of crystalline biofilms makes antibiotic treatment more difficult ( Maszewska et al, 2021 ). Proteus mirabilis has peritrichous flagella, which can carry out the typical swarming motility, called the “bull-eye” ( Aygül et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs

Liu

Dong

Ai³

et al. 2023

Front. Microbiol.

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IntroductionProteus mirabilis is a multi-host pathogen that causes diseases of varying severity in a wide range of mammals, including humans. Proteus mirabilis is resistant to multiple antibiotics and has acquired the ability to produce expanded spectrum of β-lactamases, leading to serious public health problems. However, the available information on P. mirabilis isolated from feces of dogs, is still poorly understood, as is the correlation between its virulence-associated genes (VAGs) and antibiotic resistance genes (ARGs).MethodIn this study, we isolated 75 strains of P. mirabilis from 241 samples, and investigated the swarming motility, biofilm formation, antimicrobial resistance (AMR), distribution of VAGs and ARGs, as well as the presence of class 1, 2, and 3 integrons in these isolates.ResultsOur findings suggest a high prevalence of intensive swarming motility and strong biofilm formation ability among P. mirabilis isolates. Isolates were primarily resistant to cefazolin (70.67%) and imipenem (70.67%). These isolates were found to carry ureC, FliL, ireA, zapA, ptA, hpmA, hpmB, pmfA, rsbA, mrpA, and ucaA with varying prevalence levels of 100.00, 100.00, 100.00, 98.67, 98.67, 90.67, 90.67, 90.67, 90.67, 89.33, and 70.67%, respectively. Additionally, the isolates were found to carry aac(6′)-Ib, qnrD, floR, blaCTX-M, blaCTX-M-2, blaOXA-1, blaTEM, tetA, tetB and tetM with varying prevalence levels of 38.67, 32.00, 25.33, 17.33, 16.00, 10.67, 5.33, 2.67, 1.33, and 1.33%, respectively. Among 40 MDR strains, 14 (35.00%) were found to carry class 1 integrons, 12 (30.00%) strains carried class 2 integrons, while no class 3 integrons was detected. There was a significant positive correlation between the class 1 integrons and three ARGs: blaTEM, blaCTX-M, and blaCTX-M-2. This study revealed that P. mirabilis strains isolated from domestic dogs exhibited a higher prevalence of MDR, and carried fewer VAGs but more ARGs compared to those isolated from stay dogs. Furthermore, a negative correlation was observed between VAGs and ARGs.DiscussionGiven the increasing antimicrobial resistance of P. mirabilis, veterinarians should adopt a prudent approach towards antibiotics administration in dogs to mitigate the emergence and dissemination of MDR strains that pose a potential threat to public health.

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Section: Introductionmentioning

confidence: 99%

Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs

Liu

Dong

Ai³

et al. 2023

Front. Microbiol.

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“…On the other hand, in a paper published by Moryl et al [ 26 ], the authors reported that only 5% (from 50 isolates) of sessile P. mirabilis forms were susceptible to the effects of amikacin. In other studies, AK reduced the biofilm viability of two out of the four tested P. mirabilis strains (even about 54% ± 15% for C41 isolate) [ 33 ] or even all tested 15 uropathogenic strains, completely eradicating their biofilms [ 29 ]. In contrast to the considerable antibacterial effectiveness of aminoglycosides against P. mirabilis planktonic forms, their monotherapies were less often successful in eliminating the uropathogenic sessile cells.…”

Section: Discussionmentioning

confidence: 99%

Combined Application of Aminoglycosides and Ascorbic Acid in the Elimination of Proteus mirabilis Rods Responsible for Causing Catheter-Associated Urinary Tract Infections (CAUTIs)—A Molecular Approach

Stolarek

Bernat

Różalski

2022

IJMS

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Proteus mirabilis is a common cause of catheter-associated urinary tract infections (CAUTIs). In this study, we verified the effectiveness of amikacin or gentamicin and ascorbic acid (AA) co-therapy in eliminating uropathogenic cells, as well as searched for the molecular basis of AA activity by applying chromatographic and fluorescent techniques. Under simulated physiological conditions, a combined activity of the antibiotic and AA supported the growth (threefold) of the P. mirabilis C12 strain, but reduced catheter colonization (≤30%) in comparison to the drug monotherapy. Slight modifications in the phospholipid and fatty acid profiles, as well as limited (≤62%) 2’,7’-dichlorofluorescein fluorescence, corresponding to the hydroxyl radical level, allowed for the exclusion of the hypothesis that the anti-biofilm effect of AA was related to membrane perturbations of the C12 strain. However, the reduced (≤20%) fluorescence intensity of propidium iodide, as a result of a decrease in membrane permeability, may be evidence of P. mirabilis cell defense against AA activity. Quantitative analyses of ascorbic acid over time with a simultaneous measurement of the pH values proved that AA can be an effective urine acidifier, provided that it is devoid of the presence of urease-positive cells. Therefore, it could be useful in a prevention of recurrent CAUTIs, rather than in their treatment.

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“…The results were expressed as of the percentage P. mirabilis growth inhibition. A biofilm assay was performed on the same plates according to Maszewska et al’s method [ 53 ]. After 24 h of incubation, the biofilms of P. mirabilis were washed with 0.85% NaCl to remove unbound cells.…”

Section: Methodsmentioning

confidence: 99%

Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis

et al. 2022

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The natural microbiota of the urinary tract includes Lactobacillus spp., which secrete molecules with antimicrobial properties and have antagonistic activity against many pathogens. This paper focuses on the antibacterial effect of Lactobacillus strains isolated from urine against clinical strains of Proteus mirabilis isolated from kidney stones and from urine with coexisting urolithiasis. The study involved analyzing the main antimicrobial molecules secreted by Lactobacillus. In order to indicate which agent had the strongest antimicrobial effect, the supernatants were made alkaline and treated with catalase and high temperature. Both treated and untreated supernatants were analyzed for their activity. Exposing uropathogens to all untreated cell-free supernatants of Lactobacillus significantly reduced their growth, and it was established that these properties were related to organic acid secretion by these strains. Using LC–MS/MS and spectrophotometric techniques, lactic, citric, and succinic acids were determined qualitatively and quantitatively. The influence of these acids on the P. mirabilis growth and biofilm formation and their influence on membrane permeability were also investigated. The results indicate that organic acids secreted by Lactobacillus strains have a high antibacterial potential and could be used as novel agents in the treatment of urinary tract infections caused by P. mirabilis.

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Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm

Cited by 8 publications

References 51 publications

Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs

Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs

Combined Application of Aminoglycosides and Ascorbic Acid in the Elimination of Proteus mirabilis Rods Responsible for Causing Catheter-Associated Urinary Tract Infections (CAUTIs)—A Molecular Approach

Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis

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