Amide proton transfer imaging of glioblastoma, neuroblastoma, and breast cancer cells on a 11.7 T magnetic resonance imaging system

Tanoue, Minori; Saito, Shigeyoshi; Takahashi, Yusuke; Araki, Rikita; Hashido, Takashi; Kioka, Hidetaka; Sakata, Yasushi; Yoshioka, Yoshichika

doi:10.1016/j.mri.2019.07.005

Cited by 18 publications

(17 citation statements)

References 43 publications

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“…This is further confounded in the presence of T 1 changes in diseased tissues 18 . Recently, Tanoue et al demonstrated the effect of RF saturation duration (Ts) on the CEST effect at 11.7 T 19 . It takes prolonged saturation and recovery time to reach the steady state.…”

Section: Introductionmentioning

confidence: 99%

“…17 This is further confounded in the presence of T 1 changes in diseased tissues. 18 Recently, Tanoue et al demonstrated the effect of RF saturation duration (Ts) on the CEST effect at 11.7 T. 19 It takes prolonged saturation and recovery time to reach the steady state. A trade-off has to be made between the magnitudes of the CEST effect and scan time; as a result, most CEST experiments were performed at non-steady state to expedite the scan.…”

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confidence: 99%

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Preliminary demonstration of in vivo quasi‐steady‐state CEST postprocessing—Correction of saturation time and relaxation delay for robust quantification of tumor MT and APT effects

Zhang

Zhai

Jin

et al. 2021

Magnetic Resonance in Med

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Chemical exchange saturation transfer (CEST) MRI is versatile for measuring the dilute labile protons and microenvironment properties. However, the use of insufficiently long RF saturation duration (Ts) and relaxation delay (Td) may underestimate the CEST measurement. This study proposed a quasi-steady-state (QUASS) CEST analysis for robust CEST quantification. Methods: The CEST signal evolution was modeled as a function of the longitudinal relaxation rate during Td and spin-lock relaxation rate during Ts, from which the QUASS-CEST effect is derived. Numerical simulation and in vivo rat glioma MRI experiments were conducted at 11.7 T to compare the apparent and QUASS-CEST results obtained under different Ts/Td of 2 seconds/2 seconds and 4 seconds/4 seconds. Magnetization transfer and amide proton transfer effects were resolved using a multipool Lorentzian fitting and evaluated in contralateral normal tissue and tumor regions. Results: The simulation showed the dependence of the apparent CEST effect on Ts and Td, and such reliance was mitigated with the QUASS algorithm. Animal experiment results showed that the apparent magnetization transfer and amide proton transfer effects and their contrast between contralateral normal tissue and tumor regions increased substantially with Ts and Td. In comparison, the QUASS magnetization transfer and amide proton transfer effects and their difference between contralateral normal tissue and tumor exhibited little dependence on Ts and Td. In addition, the apparent magnetization transfer and amide proton transfer were significantly smaller than the corresponding QUASS indices (P < .05). |ZHANG et Al. | INTRODUCTIONChemical exchange saturation transfer MRI has emerged as a sensitive method for detecting dilute labile protons and microenvironment properties such as pH and temperature. [1][2][3][4] It has shown promise in measuring endogenous metabolites and compounds (eg, glucose, glycogen, amide protons) and exogenous diamagnetic/paramagnetic CEST agents, offering a novel means to image a host of disorders, including acute stroke, tumor, and epilepsy. [5][6][7][8][9][10] Although CEST MRI is versatile, its contrast is quite complicated. The measurable CEST effect depends not only on the labile proton concentration and exchange rate, but also on experimental parameters, 11,12 such as the duration of RF irradiation and the relaxation delay (Td) between RF saturation. [13][14][15][16] Indeed, the CEST effect reflects two competing processes, namely, the signal reduction due to saturation transfer from irradiated labile protons and the signal recovery through relaxation. The CEST-MRI effect has a complicated dependence on bulk water T 1 . 17 This is further confounded in the presence of T 1 changes in diseased tissues. 18 Recently, Tanoue et al demonstrated the effect of RF saturation duration (Ts) on the CEST effect at 11.7 T. 19 It takes prolonged saturation and recovery time to reach the steady state. A trade-off has to be made between the magnitudes of the CEST effect and scan tim...

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Preliminary demonstration of in vivo quasi‐steady‐state CEST postprocessing—Correction of saturation time and relaxation delay for robust quantification of tumor MT and APT effects

Zhang

Zhai

Jin

et al. 2021

Magnetic Resonance in Med

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“…Like 1 H MRS, chemical exchange saturation transfer (CEST) imaging is a magnetic resonance imaging (MRI) technique for evaluating low‐concentration metabolites, but it overcomes some of the limitations of 1 H MRS 12–15 . Using prolonged and selective saturation pulses, CEST imaging can evaluate metabolites with exchangeable protons such as amide and amine protons.…”

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confidence: 99%

Accurate Estimation of the Duration of Testicular Ischemia Using Creatine Chemical Exchange Saturation Transfer (CrCEST) Imaging

Takahashi

Kioka

Saito

et al. 2020

Magnetic Resonance Imaging

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Background In the management of testicular torsion, estimating the duration of testicular ischemia is essential for deciding on an appropriate surgical treatment, but there are currently limited evaluation methods. Purpose To perform testicular creatine chemical exchange saturation transfer (CrCEST) imaging and to evaluate its ability to accurately estimate the duration of testicular ischemia. Study Type Prospective. Animal Model C57BL/6 control mice (n = 6) and testicular ischemia models induced by clamping the spermatic cord (n = 14). Eight of testicular ischemia models were serially imaged at two or three timepoints and a total of 26 images of ischemic testis were obtained. The ischemic duration ranged from 6–42 hours. Field Strength/Sequence 11.7T vertical‐bore MRI/segment fast low‐angle shot acquisition for CEST. Assessment CrCEST imaging was performed and the magnetization transfer ratio for the CrCEST effect (MTRCr**) was calculated in control mice and testicular ischemia models. Correlation analysis between the duration of testicular ischemia and MTRCr** decline was performed. Statistical Tests Paired t‐test, and Pearson's correlation analysis. Results In control mice, the CrCEST effect in testes was significantly more than five times higher than that in skeletal muscle. MTRCr** did not differ significantly between the right and left testes (8.6 ± 0.8 vs. 8.3 ± 0.6, P = 0.96). In testicular ischemia models, MTRCr** of ischemic testes was significantly lower than that of controls (4 ± 2 vs. 8.9 ± 0.6, P < 0.001). Correlation analysis revealed a strong linear correlation between MTRCr** decline and the duration of ischemia (r = 0.96, P < 0.001). Data Conclusion A decreased CrCEST effect in ischemic testes correlated well with ischemic duration. Testicular CrCEST imaging was useful for accurately estimating the duration of testicular ischemia. Level of Evidence 2 Technical Efficacy Stage 2

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“…This disagreement shows that the effect of CEST is different from the mechanism generating the signals on DWI. DWI detects water movement inside and outside the cell, but CEST can detect metabolites, including APT, 15 Cr, 16 and Lac. 17 Therefore, CEST can observe the changes in in vivo metabolites caused by hypoxia-ischemia that cannot be detected with DWI.…”

Section: Discussionmentioning

confidence: 99%

“…Chemical exchange saturation transfer (CEST) is a new imaging technology introduced in 2000. 11 To evaluate animal models, compounds containing exchangeable endogenous protons, such as glutamate, 12 creatine, [13][14][15][16] lactate, 17,18 and other metabolites, 15 have been imaged by CEST. Zheng and Wang 8 applied amide proton transfer (APT) imaging to neonatal piglets.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Chemical Exchange Saturation Transfer Imaging with Diffusion-weighted Imaging and Magnetic Resonance Spectroscopy in a Rat Model of Hypoxic-ischemic Encephalopathy

et al. 2020

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This study aimed to evaluate the effect of chemical exchange saturation transfer (CEST) on the ischemic regions in hypoxic-ischemic encephalopathy (HIE) in comparison with diffusion-weighted imaging (DWI) and magnetic resonance spectroscopy (MRS) using a 7T-MRI. Methods: We used neonatal rats (n = 8), aged 8 days, to clarify the progression of HIE. The rat model of HIE was developed by ligating and severing the left common carotid artery, followed by 45 minutes of recovery, and 60 minutes of hypoxia (8% O 2 /92% N 2 ; 34°C). At 0-2 and 24 hours after the onset of HIE, CEST imaging, DWI, and MRS were performed with a 7T-MRI. The magnetization transfer ratio (MTR) asymmetry curves and four MTR asymmetry maps at 0.5, 1.0, 2.0, and 3.5 ppm were calculated using the CEST images. Fractional anisotropy (FA) and apparent diffusion coefficient (ADC) maps were calculated by DWI, and brain metabolites were assessed by MRS. Results: In the ischemic regions of neonatal rats, FA was significantly increased at 0-2 hours and decreased at 24 hours after the onset of HIE. ADC in the ipsilateral side was significantly lower than that of contralateral side. All rats with HIE showed hypointense areas on MTR asymmetry maps (2.0 and 3.5 ppm), that did not correspond with the hyperintense areas on DWI. In addition, a significant increase in lactate levels was observed at 0-2 and 24 hours after the onset of HIE. Conclusion: CEST MTR maps did not correspond with the hyperintense areas on DWI at 0-2 and 24 hours after the onset of HIE. The change of multi offset CEST signal may be primarily related to the brain metabolites and pH alterations, such as that caused by lactate, after the onset of HIE.

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Amide proton transfer imaging of glioblastoma, neuroblastoma, and breast cancer cells on a 11.7 T magnetic resonance imaging system

Cited by 18 publications

References 43 publications

Preliminary demonstration of in vivo quasi‐steady‐state CEST postprocessing—Correction of saturation time and relaxation delay for robust quantification of tumor MT and APT effects

Preliminary demonstration of in vivo quasi‐steady‐state CEST postprocessing—Correction of saturation time and relaxation delay for robust quantification of tumor MT and APT effects

Accurate Estimation of the Duration of Testicular Ischemia Using Creatine Chemical Exchange Saturation Transfer (CrCEST) Imaging

Comparison of Chemical Exchange Saturation Transfer Imaging with Diffusion-weighted Imaging and Magnetic Resonance Spectroscopy in a Rat Model of Hypoxic-ischemic Encephalopathy

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Amide proton transfer imaging of glioblastoma, neuroblastoma, and breast cancer cells on a 11.7 T magnetic resonance imaging system

Cited by 18 publications

References 43 publications

Preliminary demonstration of in vivo quasi‐steady‐state CEST postprocessing—Correction of saturation time and relaxation delay for robust quantification of tumor MT and APT effects

Preliminary demonstration of in vivo quasi‐steady‐state CEST postprocessing—Correction of saturation time and relaxation delay for robust quantification of tumor MT and APT effects

Accurate Estimation of the Duration of Testicular Ischemia Using Creatine Chemical Exchange Saturation Transfer (CrCEST) Imaging

Comparison of Chemical Exchange Saturation Transfer Imaging with Diffusion-weighted Imaging and Magnetic Resonance Spectroscopy in a Rat Model of Hypoxic-ischemic Encephalopathy

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Amide proton transfer imaging of glioblastoma, neuroblastoma, and breast cancer cells on a 11.7 T magnetic resonance imaging system