American Visceral Leishmaniasis: The Etiological Agent

Herpetomonas megaseliae and Crithidia harmosa: Growth on Blood-Agar Plates

“…In addition, the observed differences in colony morphology can be attributed at least to the generic differences between the two species, and thus may have a genetic basis. Although agar plate cultures of Trichomonas species have been used for many years, both for cloning and diagnosis (Asami et al, 1955;Kulda et al, 1970), and blood-agar slants or overlaid blood-agar media have been used for many years in the culture of trypanosomatids (Nbller, 1917;Senekjie, 1944;Newton, 1956), this paper (1) expands the list of trypanosomatid species to which these techniques are applicable and (2) re-emphasizes the techniques' potential use as a research tool. The latter point was apparently largely overlooked by these previous workers (N6ller, 1917;Newton, 1956).…”

Isolation and Characterization of Drug Resistant Mutants of Crithidia fasciculata

“…coli (Riou and Pautrizel, 1969;Klotz and Zimm, 1972;Wesley and Simpson, 1973;Leon et al, 1978;Borst et al, 1980;Castro et al, 1981;Liinar et al, 1981). They can be grown to high cell densities (> 10''/ml) often in defined chemical media (Newton, 1956;Trager, 1957;Kidder and Dutta, 1958), and can be grown on agar (Noller, 1917;Senekije, 1944;Simpson, 1968;Wagner and Krassner, 1976;Keppel andJanovy, 1977, 1980) with plating efficiencies of 30 to 100%. However, one major drawback is the lack of the availability of a hemoflagellate genetics system.…”