American Trypanosomiasis (Chagas’ Disease)

Camargo, M. E.

doi:10.1007/978-1-4612-3898-0_77

Cited by 11 publications

(3 citation statements)

References 20 publications

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“…In the serologicai diagnosis of Chagas" disease, the use of conventional reactions with complex antigenic mixtures presents high cross-reactivity with leishmaniasis patients, as reported by several authors [5][6][7][8][9]. In some laboratories, it is usual to work with high dilutions of both chagasic and leishmaniasic sera in an attempt to avoid eross-reactivity.…”

Section: Discussionmentioning

confidence: 99%

Cross-reactivity studies and differential serodiagnosis of human infections caused by Trypanosoma cruzi and Leishmania spp; use of immunoblotting and ELISA with a purified antigen (Ag163B6)

Malchiodi

Chiaramonte

Taranto³

et al. 1994

Clinical and Experimental Immunology

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SUMMARY Results of our studies on the reactivity of chagasic and leishmaniasic sera with the purified T. cruzi‐ specific antigen 163B6, as assessed by ELISA, and with complex antigenic mixtures from T. cruzi and Leishmania mexicana, by immunoblotting. are presented here. Our objective was to identify the antigens responsible for the exhibited cross‐reactivity between trypanosomiasis and leishmaniasis, and to find a specific reactivity pattern corresponding to each parasitosis. In spite of the high cross‐reactivity observed with the immunoblotting, the use of 7.5% A‐B gels made it possible to identify a characteristic pattern for each parasitosis, that could be distinguished by the naked eye. The characteristic pattern corresponding to chagasic patients was ascribed to reactivity with T, cruzi bands of mol. wts 131, 125, 116, 111, 51‐45 and 43 kD, that were not recognized by leishmaniasic sera. Trypanosoma cruzi antigens of mol. wts 85, 81, 70, 65 60, 37 and 32kD were considered as crossing antigens, since they were recognized by leishmaniasis sera. With L. mexicana, most of the chagasic patients presented reaction with antigen of mol. wts 124, 107. 92, 59 and 32kD, while bands of mol. wts 155, 140. 73. 56 and 48kD were recognized only by leishmaniasic sera. In this study we found 12 out of 45 sera of patients with leishmaniasis, from a region endemic for both parasitoses, which exhibited a pattern of bands very similar to those corresponding to chagasic individuals, strongly suggesting a mixed infection. This hypothesis was verified by using a purified specific antigen of T. cruzi, Ag163B6, which would be the major cysteine proteinase of this specie (cruzipain). By HLISA, these 12 sera showed a positive reaction with this purified antigen, as those of chagasic patients, thus leading to the confirmation of the presence of a mixed infection.

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Section: Discussionmentioning

confidence: 99%

Cross-reactivity studies and differential serodiagnosis of human infections caused by Trypanosoma cruzi and Leishmania spp; use of immunoblotting and ELISA with a purified antigen (Ag163B6)

Malchiodi

Chiaramonte

Taranto³

et al. 1994

Clinical and Experimental Immunology

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“…O diagnóstico diferencial com outras doenças (por exemplo, leishmaniose visceral, hanseníase na forma clínica virchowiana, doenças autoimunes, entre outras) deve ser considerado. [140][141][142]…”

Section: Critério Parasitológicounclassified

II Consenso Brasileiro em Doença de Chagas, 2015

Dias¹,

Ramos²,

Gontijo³

et al. 2016

Epidemiol. Serv. Saúde

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A doença de Chagas é uma condição crônica negligenciada com elevada carga de morbimortalidade e impacto dos pontos de vista psicológico, social e econômico. Representa um importante problema de saúde pública no Brasil, com diferentes cenários regionais. Este documento traduz a sistematização das evidências que compõe o Consenso Brasileiro de Doença de Chagas. O objetivo foi sistematizar estratégias de diagnóstico, tratamento, prevenção e controle da doença de Chagas no país, de modo a refletir as evidências científicas disponíveis. Sua construção fundamentou-se na articulação e contribuição estratégica de especialistas brasileiros com conhecimento, experiência e atualização sobre diferentes aspectos da doença. Representa o resultado da estreita colaboração entre a Sociedade Brasileira de Medicina Tropical e o Ministério da Saúde. Espera-se com este documento fortalecer o desenvolvimento de ações integradas para enfrentamento da doença no país com foco em epidemiologia, gestão, atenção integral (incluindo famílias e comunidades), comunicação, informação, educação e pesquisas.

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“…A number of highly sensitive serological assays are used in Latin America for detecting anti-T. cruzi antibodies, such as the complement fixation and indirect immunofluorescence tests and the enzyme-linked immunosorbent assay (ELISA) (36). These and other conventional serologic assays are used widely for clinical diagnosis and for screening donated blood, as well as in epidemiological studies.…”

Section: Downloaded Frommentioning

confidence: 99%

Chagas' disease

et al. 1992

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Chagas' disease, caused by Trypanosoma cruzi, is an important cause of morbidity in many countries in Latin America. The important modes of transmission are by the bite of the reduviid bug and blood transfusion. The organism exists in three morphological forms: trypomastigotes, amastigotes, and epimastigotes. The mechanism of transformation and differentiation is currently being explored, and signal transduction pathways of the parasites may be involved in this process. Parasite adherence to and invasion of host cells is a complex process involving complement, phospholipase, penetrin, neuraminidase, and hemolysin. Two clinical forms of the disease are recognized, acute and chronic. During the acute stage pathological damage is related to the presence of the parasite, whereas in the chronic stage few parasites are found. In recent years the roles of tumor necrosis factor, gamma interferon, and the interleukins in the pathogenesis of this infection have been reported. The common manifestations of chronic cardiomyopathy are arrhythmias and thromboembolic events. Autoimmune, neurogenic, and microvascular factors may be important in the pathogenesis of the cardiomyopathy. The gastrointestinal tract is another important target, and "mega syndromes" are common manifestations. The diagnosis and treatment of this infection are active areas of investigation. New serological and molecular biological techniques have improved the diagnosis of chronic infection. Exacerbations of T. cruzi infection have been reported for patients receiving immuno-suppressive therapy and for those with AIDS.

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American Trypanosomiasis (Chagas’ Disease)

Cited by 11 publications

References 20 publications

Cross-reactivity studies and differential serodiagnosis of human infections caused by Trypanosoma cruzi and Leishmania spp; use of immunoblotting and ELISA with a purified antigen (Ag163B6)

Cross-reactivity studies and differential serodiagnosis of human infections caused by Trypanosoma cruzi and Leishmania spp; use of immunoblotting and ELISA with a purified antigen (Ag163B6)

II Consenso Brasileiro em Doença de Chagas, 2015

Chagas' disease

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