American Bison (Bison bison) reproductive endocrinology: serum Pregnancy Associated Glycoproteins (PAG), Progesterone, Estrone and Estrone-Sulfate in non pregnant animals and during gestation

Frisee, Vincent; Rigaux, Goulven; Dufour, Patrice; Barbato, Olimpia; Brutinel, Flore; Egyptien, Sophie; Bossaert, Philippe; Deleuze, Stéfan; Cavalier, Étienne; Ponthier, Jérôme

doi:10.1016/j.domaniend.2021.106684

Cited by 7 publications

(9 citation statements)

References 40 publications

(57 reference statements)

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“…As was to be expected, both PAG and P4 concentrations showed a higher value in pregnant animals than in non-pregnant animals [15,21,53]. The results of this study confirm PAG determination as a reliable method for pregnancy diagnosis and the follow up of trophoblastic function.…”

Section: Discussionsupporting

confidence: 86%

Interferon-Tau in Maternal Peripheral Blood and Its Relationship with Progesterone and Pregnancy-Associated Glycoproteins in the Early Phases of Gestation in Water Buffalo

Barbato,

Menchetti,

Casano

et al. 2024

Animals

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The aim of this study was to investigate the interferon tau (IFNt) concentration in the peripheral maternal blood during the early phase of pregnancy in buffalo cows and improve the knowledge on the physiological importance of circulating IFNt, evaluating the possible interaction with pregnancy-associated glycoproteins (PAGs) and progesterone (P4). Blood samples were taken from buffalo cows on day 0 (day of AI), 7, 14, 18, 28, and 40 post insemination for the IFNt, PAG, and P4 analysis and to determine the IFNt mRNA expression. The animals were categorized ex post into Pregnant, Non-pregnant and Embryo mortality groups. The interferon value was influenced by group (p = 0.003), being always higher in pregnant buffalo cows than in non-pregnant ones, while the embryo mortality group showed intermediate values between those for pregnant and non-pregnant animals. The mRNA expression of IFNt was not influenced by groups or any time points. The regression analysis that included IFNt as the independent variable showed that PAGs, from day 18 (p < 0.01), and P4, from day 28 (p < 0.05), were positively associated with IFNt values. The close associations among IFNt, PAGs and P4 demonstrate that all three molecules work together for fetal–placental well-being and pregnancy support. Unfortunately, the great individual variability in circulating IFNt makes this analysis unsuitable for early pregnancy diagnosis.

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Section: Discussionsupporting

confidence: 86%

Interferon-Tau in Maternal Peripheral Blood and Its Relationship with Progesterone and Pregnancy-Associated Glycoproteins in the Early Phases of Gestation in Water Buffalo

Barbato,

Menchetti,

Casano

et al. 2024

Animals

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“…Overall, estrogens were analyzed in plasma, serum, urine, and tissue samples. The sample work-up for blood or tissue samples prior to LC-MS/MS analysis omitted any LLE- or SPE-based sample preparation methods [ 61 , 62 , 63 ], whereas the reviewed sample clean-up procedures prior to GC-MS analysis applied SPE-based protocols [ 64 , 65 ].…”

Section: Steroidsmentioning

confidence: 99%

Section: Steroidsmentioning

confidence: 99%

“…In the study reported by Frisée et al [ 62 ], serum samples were tested for both estrogens and progesterone. The samples were centrifuged and then stored at −80 °C until the LC-MS analysis [ 62 ]. The LOQ was reported to be 0.1 ng/mL for progesterone, 0.002 ng/mL for estrone, and 0.5 ng/mL for estrone-sulfate.…”

Section: Steroidsmentioning

confidence: 99%

“… Chromatography Ionization Detection Monitoring Mode Column Temperature [°C] Flow Rate [mL/min] Mobile Phase A Mobile Phase B Reference LC ESI − TQ MRM XDB-Phenyl (50 mm × 2.1 mm, 5 µm) 45 0.6 5 mM ammonium fluoride aq MeOH [ 61 ] LC ESI +− QTrap MRM C18 (100 mm × 2.1 mm, 1.7 µm) n.a. 0.4 0.02% NH 4 OH in water ACN [ 62 ] LC ESI +− QTrap MRM C18 (100 mm × 2.1 mm, 1.7 µm) n.a. 0.4 0.02% NH 4 OH aq ACN [ 63 ] …”

Section: Table A1mentioning

confidence: 99%

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Sample Preparation Techniques for Growth-Promoting Agents in Various Mammalian Specimen Preceding MS-Analytics

Klöppner,

Harps,

Parr

2024

Molecules

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The misuse of growth-promoting drugs such as beta-2 agonists and steroids is a known problem in farming and sports competitions. Prior to the analysis of biological samples via liquid chromatography (LC)–mass spectrometry (MS) or gas chromatography (GC)–MS, sufficient sample preparation is required to reliably identify or determine the residues of drugs. In practice, broad screening methods are often used to save time and analyze as many compounds as possible. This review was conceptualized to analyze the literature from 2018 until October 2023 for sample preparation procedures applied to animal specimens before LC- or GC-MS analysis. The animals were either used in farming or sports. In the present review, solid phase extraction (SPE) was observed as the dominant sample clean-up technique for beta-2 agonists and steroids, followed by protein precipitation. For the extraction of beta-2 agonists, mixed-mode cation exchanger-based SPE phases were preferably applied, while for the steroids, various types of SPE materials were reported. Furthermore, dispersive SPE-based QuEChERs were utilized. Combinatory use of SPE and liquid–liquid extraction (LLE) was observed to cover further drug classes in addition to beta-2 agonists in broader screening methods.

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Occurrence, analysis and removal of pesticides, hormones, pharmaceuticals, and other contaminants in soil and water streams for the past two decades: a review

Kanan

Moyet²,

Obeideen³

et al. 2022

Res Chem Intermed

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American Bison (Bison bison) reproductive endocrinology: serum Pregnancy Associated Glycoproteins (PAG), Progesterone, Estrone and Estrone-Sulfate in non pregnant animals and during gestation

Cited by 7 publications

References 40 publications

Interferon-Tau in Maternal Peripheral Blood and Its Relationship with Progesterone and Pregnancy-Associated Glycoproteins in the Early Phases of Gestation in Water Buffalo

Interferon-Tau in Maternal Peripheral Blood and Its Relationship with Progesterone and Pregnancy-Associated Glycoproteins in the Early Phases of Gestation in Water Buffalo

Sample Preparation Techniques for Growth-Promoting Agents in Various Mammalian Specimen Preceding MS-Analytics

Occurrence, analysis and removal of pesticides, hormones, pharmaceuticals, and other contaminants in soil and water streams for the past two decades: a review

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