Amended Safety Assessment of Dodecylbenzenesulfonate, Decylbenzenesulfonate, and Tridecylbenzenesulfonate Salts as Used in Cosmetics

Becker, Lillian C.; Bergfeld, Wilma F.; Belsito, D.; Hill, Ronald A.; Klaassen, Curtis D.; Liebler, Daniel C.; Marks, James G.; Shank, Ronald C.; Slaga, Thomas J.; Snyder, Paul W.; Andersen, F. Alan

doi:10.1177/1091581810386124

Cited by 13 publications

(6 citation statements)

References 31 publications

(42 reference statements)

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“…This is also borne out by the continued growth of cells on the scaffolds, which do not appear to be adversely affected by the presence of DBSA or indeed the potential for its presence in the surrounding media. DBSA is a surfactant used in some cosmetics, therefore, cytotoxic effects may generally be those of a detergent which can be tolerated by cells at low concentrations . The stability of the conductivity of DBSA‐containing scaffolds speak to the excellent compositional properties of the scaffolds and their future application for longer‐term monitoring of cell cultures.…”

Section: Assessing Electrical Properties Of Pedot:pss Scaffolds Beformentioning

confidence: 99%

Conducting Polymer Scaffolds for Hosting and Monitoring 3D Cell Culture

et al. 2017

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now clear that architectures that reproduce tissue organization in 3D are favored for studying function as first shown by Bissell and co-workers nearly 30 years ago. [5,6] Another emerging concept is the capacity for multiple cell types to auto-organize when given the appropriate mechanical cues. [7] A large variety of 3D models have been developed, with the major subtypes being spheroids/organoids, multilayered tissue like models, and scaffold models. [8] 3D tissues are frequently constructed as spheroids using hydrogel-like matrices such as Matrigel, [9] or PuraMatrix, [10] with a highly desirable degree of mechanical softness, however, there can be problems related to cost and inhomogeneity of the materials. [11] Multilayer models hold a lot of promise for applications such as skin toxicology measurements, [12] however, they cannot be easily applied for more complex tissue or organ constructs including vasculature. Considerable attention has thus focused on the development of biocompatible scaffold materials for hosting cells in 3D. A variety of synthetic and bio-derived polymers (some resorbable, some not) have been used to mimic the ECM or connective tissue. In terms of technology integration, the major advances so far for 3D cell biology have been related to materials and methods used for scaffold preparation, and integration of microfabrication techniques, for example for fluidics. As might be expected, a challenge of 3D culture over 2D is associated with the difficulty of oxygenation of tissues in the absence of vasculature. Microfluidics have gained favor for a number of reasons, including for perfusion, reduction in reagent volumes, and the fact that flow induced stress This work reports the design of a live-cell monitoring platform based on a macroporous scaffold of a conducting polymer, poly(3,4-ethylene dioxythiophene):poly(styrenesulfonate). The conducting polymer scaffolds support 3D cell cultures due to their biocompatibility and tissue-like elasticity, which can be manipulated by inclusion of biopolymers such as collagen. Integration of a media perfusion tube inside the scaffold enables homogenous cell spreading and fluid transport throughout the scaffold, ensuring long term cell viability. This also allows for co-culture of multiple cell types inside the scaffold. The inclusion of cells within the porous architecture affects the impedance of the electrically conducting polymer network and, thus, is utilized as an in situ tool to monitor cell growth. Therefore, while being an integral part of the 3D tissue, the conducting polymer is an active component, enhancing the tissue function, and forming the basis for a bioelectronic device with integrated sensing capability.

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Section: Assessing Electrical Properties Of Pedot:pss Scaffolds Beformentioning

confidence: 99%

Conducting Polymer Scaffolds for Hosting and Monitoring 3D Cell Culture

et al. 2017

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“…However, benzalkonium chloride belongs to the cationic surfactants group of quaternary ammonium compounds that are found to be highly toxic to different aquatic organisms as well as being capable of inducing genotoxic effects in mammalian and plant cells [43]. Similarly, sodium dodecylbenzene sulfonate was reported to cause severe irritancy in rabbit skin at a concentration of 15% [44]. With the appearance and prevalence of multi-drug resistant microorganisms in the hospital settings along with the toxicity issues related to widely employed commercial disinfectants, the exploration of alternative antimicrobial agents from other sources, especially from plants is in high demand.…”

Section: Discussionmentioning

confidence: 99%

“…coli (BL21) were transformed with pT3-5-LO plasmid, and recombinant 5-LO protein was expressed and partially purified as described [50]. In brief, cells were lysed in 50 mM triethanolamine/HCl pH 8.0, 5 mM EDTA, soybean trypsin inhibitor (60 µg/mL), 1 mM phenyl-methanesulphonyl fluoride, and lysozyme (1 mg/mL), homogenized by sonication (3 × 15 s), and centrifuged at 40,000× g for 20 min at 4 • C. The 40,000× g supernatant was applied to an ATP-agarose column to partially purify 5-LO as described [44]. Aliquots of semi-purified 5-LO were immediately diluted with ice-cold PBS containing 1 mM EDTA, and 1 mM ATP was added.…”

Section: -Lo Activity In Cell-free Assaysmentioning

confidence: 99%

The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri Lanka

et al. 2020

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Traditional folk medicine in Sri Lanka is mostly based on plants and plant-derived products, however, many of these medicinal plant species are scientifically unexplored. Here, we evaluated the anti-inflammatory and antimicrobial potency of 28 different extracts prepared from seven popular medicinal plant species employed in Sri Lanka. The extracts were subjected to cell-based and cell-free assays of 5-lipoxygenase (5-LO), microsomal prostaglandin E2 synthase (mPGES)-1, and nitric oxide (NO) scavenging activity. Moreover, antibacterial and disinfectant activities were assessed. Characterization of secondary metabolites was achieved by gas chromatography coupled to mass spectrometric (GC-MS) analysis. n-Hexane- and dichloromethane-based extracts of Garcinia cambogia efficiently suppressed 5-LO activity in human neutrophils (IC50 = 0.92 and 1.39 µg/mL), and potently inhibited isolated human 5-LO (IC50 = 0.15 and 0.16 µg/mL) and mPGES-1 (IC50 = 0.29 and 0.49 µg/mL). Lipophilic extracts of Pothos scandens displayed potent inhibition of mPGES-1 only. A methanolic extract of Ophiorrhiza mungos caused significant NO scavenging activity. The lipophilic extracts of G. cambogia exhibited prominent antibacterial and disinfectant activities, and GC-MS analysis revealed the presence of fatty acids, sesquiterpenes and other types of secondary metabolites. Together, our results suggest the prospective utilization of G. cambogia as disinfective agent with potent anti-inflammatory properties.

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“…A single occluded dose of ∼20 mg/cm 2 (1,500 mg/kg) [ 14 C]diethanolamine was applied to a 19.5 cm 2 area on the back of rats for 6 hours, and the treated site of half of the animals was rinsed after the occlusive wrapping was removed. 26 In the animals that were not rinsed, 80% of the dose was found in the wrappings and 3.6% found in the skin.…”

Section: Toxicokineticsmentioning

confidence: 99%

“…In 2009, the Expert Panel reviewed the safety of DEA-dodecylbenzenesulfonate, an ingredient included in this rereview. 2 It was concluded that DEA-dodecylbenzenesulfonate is safe as used when formulated to be nonirritating.…”

Section: Introductionmentioning

confidence: 99%

Safety Assessment of Diethanolamine and Its Salts as Used in Cosmetics

et al. 2017

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The Cosmetic Ingredient Review (CIR) Expert Panel assessed the safety of diethanolamine and its salts as used in cosmetics. Diethanolamine functions as a pH adjuster; the 16 salts included in this rereview reportedly function as surfactants, emulsifying agents, viscosity increasing agents, hair or skin conditioning agents, foam boosters, or antistatic agents. The Panel reviewed available animal and clinical data, as well as information from previous CIR reports. Since data were not available for each individual ingredient, and since the salts dissociate freely in water, the Panel extrapolated from previous reports to support safety. The Panel concluded that diethanolamine and its salts are safe for use when formulated to be nonirritating. These ingredients should not be used in cosmetic products in which N-nitroso compounds can be formed.

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Amended Safety Assessment of Dodecylbenzenesulfonate, Decylbenzenesulfonate, and Tridecylbenzenesulfonate Salts as Used in Cosmetics

Cited by 13 publications

References 31 publications

Conducting Polymer Scaffolds for Hosting and Monitoring 3D Cell Culture

Conducting Polymer Scaffolds for Hosting and Monitoring 3D Cell Culture

The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri Lanka

Safety Assessment of Diethanolamine and Its Salts as Used in Cosmetics

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