Amelogenin-Cytokeratin 14 Interaction in Ameloblasts during Enamel Formation

Ravindranath, Rajeswari M.H.; Tam, Wai-Yin; Bringas, Pablo; Valentino, Salvatore; Fincham, Alan G.

doi:10.1074/jbc.m104656200

Cited by 34 publications

(45 citation statements)

References 50 publications

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“…A GMp motif is found in the N-terminal region of Type I cytokeratin 14 (K14), a differentiation marker for ameloblasts prior to amelogenin synthesis (7). The purified or recombinant amelogenin and TRAP bind to K14 in vitro dosimetrically (8). Scatchard analysis confirms the specific interaction between K14 and ATMP in vitro.…”

mentioning

confidence: 68%

“…The K14-amelogenin complex migrated to the apical region of the ameloblasts on day 1 and accumulated there between days 3 and 5 and collapsed on day 9. Autoradiography with [ 3 H]ATMP and [ 3 H]GMp corroborated the dissociation of amelogenin and K14 at the Tomes' process of the ameloblast, suggesting that K14 plays a chaperone role for nascent amelogenin polypeptide during secretion of amelogenin (8).…”

mentioning

confidence: 83%

“…A total of 25 normal, healthy, female pregnant Swiss Webster mice (Charles River Breeding) were used to obtain a sufficient number of litters as was carried out previously (8). The Institutional Animal Care and Use Committee (Los Angeles, CA) approved all protocols involving mice.…”

Section: Methodsmentioning

confidence: 99%

“…3 H Labeling of ATMP-The 13-residue ATMP "P[ 3 H]YPSYGYE-PMGGW" was prepared and purified by Amersham Biosciences as mentioned previously (8).…”

Section: Methodsmentioning

confidence: 99%

“…Western Blot Analysis-K5 purified from HeLa cells and proteins isolated from ameloblast extracts during different developmental days (newborn to PN day 9) were resolved by gradient SDS-PAGE (4 -12%) and electrotransferred to polyvinylidene difluoride (PVDF) membranes (Immobilon-P Transfer Membrane, Millipore Corp.) at 100 mA for 35 min using a semi-dry transblot apparatus (Bio-Rad Scientific Instruments) (5,8). Protein transfer was assessed by staining the strips with 0.1% Fast Green (Sigma) in 40% methanol and 10% acetic acid (5,8).…”

Section: Methodsmentioning

confidence: 99%

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Amelogenin Interacts with Cytokeratin-5 in Ameloblasts during Enamel Growth

Ravindranath

Basilrose

Ravindranath

et al. 2003

Journal of Biological Chemistry

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mentioning

confidence: 68%

mentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

“…3 H Labeling of ATMP-The 13-residue ATMP "P[ 3 H]YPSYGYE-PMGGW" was prepared and purified by Amersham Biosciences as mentioned previously (8).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Amelogenin Interacts with Cytokeratin-5 in Ameloblasts during Enamel Growth

Ravindranath

Basilrose

Ravindranath

et al. 2003

Journal of Biological Chemistry

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Quiescent epithelial cell rests of Malassez can differentiate into ameloblast‐like cells

Shinmura

Tsuchiya

Hata³

et al. 2008

Journal Cellular Physiology

103

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Epithelial cell rests of Malassez (ERM) are quiescent epithelial remnants of Hertwig's epithelial root sheath (HERS) that are involved in the formation of tooth roots. After completion of crown formation, HERS are converted from cervical loop cells, which have the potential to generate enamel for tooth crown formation. Cervical loop cells have the potential to differentiate into ameloblasts. Generally, no new ameloblasts can be generated from HERS, however this study demonstrated that subcultured ERM can differentiate into ameloblast-like cells and generate enamel-like tissues in combination with dental pulp cells at the crown formation stage. Porcine ERM were obtained from periodontal ligament tissue by explant culture and were subcultured with non-serum medium. Thereafter, subcultured ERM were expanded on 3T3-J2 feeder cell layers until the tenth passage. The in vitro mRNA expression pattern of the subcultured ERM after four passages was found to be different from that of enamel organ epithelial cells and oral gingival epithelial cells after the fourth passage using the same expansion technique. When subcultured ERM were combined with subcultured dental pulp cells, ERM expressed cytokeratin14 and amelogenin proteins in vitro. In addition, subcultured ERM combined with primary dental pulp cells seeded onto scaffolds showed enamel-like tissues at 8 weeks post-transplantation. Moreover, positive staining for amelogenin was observed in the enamel-like tissues, indicating the presence of well-developed ameloblasts in the implants. These results suggest that ERM can differentiate into ameloblast-like cells.

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Expression patterns of keratin family members during tooth development and the role of keratin 17 in cytodifferentiation of stratum intermedium and stellate reticulum

Inada,

Chiba,

Tian

et al. 2024

Journal Cellular Physiology

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Keratins are typical intermediate filament proteins of the epithelium that exhibit highly specific expression patterns related to the epithelial type and stage of cellular differentiation. They are important for cytoplasmic stability and epithelial integrity and are involved in various intracellular signaling pathways. Several keratins are associated with enamel formation. However, information on their expression patterns during tooth development remains lacking. In this study, we analyzed the spatiotemporal expression of keratin family members during tooth development using single‐cell RNA‐sequencing (scRNA‐seq) and microarray analysis. scRNA‐seq datasets from postnatal Day 1 mouse molars revealed that several keratins are highly expressed in the dental epithelium, indicating the involvement of keratin family members in cellular functions. Among various keratins, keratin 5 (Krt5), keratin 14 (Krt14), and keratin 17 (Krt17) are highly expressed in the tooth germ; KRT17 is specifically expressed in the stratum intermedium (SI) and stellate reticulum (SR). Depletion of Krt17 did not affect cell proliferation in the dental epithelial cell line SF2 but suppressed their differentiation ability. These results suggest that Krt17 is essential for SI cell differentiation. Furthermore, scRNA‐seq results indicated that Krt5, Krt14, and Krt17 exhibited distinct expression patterns in ameloblast, SI, and SR cells. Our findings contribute to the elucidation of novel mechanisms underlying tooth development.

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Amelogenin-Cytokeratin 14 Interaction in Ameloblasts during Enamel Formation

Abstract: The enamel protein amelogenin binds to the GlcNAcmimicking peptide (GMp) (Ravindranath, R. M. H., Tam

Cited by 34 publications

References 50 publications

Amelogenin Interacts with Cytokeratin-5 in Ameloblasts during Enamel Growth

Amelogenin Interacts with Cytokeratin-5 in Ameloblasts during Enamel Growth

Quiescent epithelial cell rests of Malassez can differentiate into ameloblast‐like cells

Expression patterns of keratin family members during tooth development and the role of keratin 17 in cytodifferentiation of stratum intermedium and stellate reticulum

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