Ameliorative role of zinc on cypermethrin-induced changes in haematological parameters and oxidative stress biomarkers in rat erythrocytes

Das, Tuhina; Pradhan, Ananya; Paramanik, Anurag; Choudhury, Sujata Maiti

doi:10.1007/s13530-016-0280-2

Cited by 13 publications

(13 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MDA is a main metabolite of lipid peroxidation that is brought by reactive oxygen species and therefore may designate the degree of oxidative stress present. Aouey et al [38] and AlKahtane,et al, [39] stated that the neurotoxic effect of CYP occur through lipid perioxidation and other oxidative damages.…”

Section: Discussionmentioning

confidence: 99%

Amira Ebrahim Alsemeh, Mohamed A. M. AbdelRahman, Fatma A. Mahmoud, Eman Ramadan Abd El Fattah

Alsemeh

Abdel-Rahman

Mahmoud

et al. 2020

Journal of Medical Histology

View full text Add to dashboard Cite

Cypermethrin (CYP) is a synthetic pyrethroid. It crosses the blood-brain barrier in pests and creates neurotoxicity in the CNS. Hydroxytyrosol (HT) is considered a powerful natural antioxidant, it is found in olive leaf, olive pulp and olive oil. Hydroxytyrosol is the only phenol that is capable to pass the blood-brain barrier and permits it to involve free radicals throughout the CNS. We designed this study to explore the possible protective role of HT against the neurotoxic effect induced by CYP. Thirty male adult albino rats were divided into three groups. Control group; subdivided into: control, corn oil, HT groups, CYP treated and CYP, CYP+ HT treated group. Treatments were given by gavage for 14 days. The cerebrum tissue was prepared for histopathological examination and immunohistochemical detection of caspase 3 and GFAP. We also assessed the level of GAPA and ACH in the tissue and the oxidative enzymatic and the lipid perioxidation profile. We observed that the concomitant use of HT with the CYP diminished the signs of hyper excitability induced by the CYP which confirmed by significant decrease in the level of GABA in cerebrum tissue. HT counteracted the histopathological changes induced by CYP and significantly downregulated the caspase -3 and GFAP expression in cerebrum tissue. A rescue of the oxidative enzymatic activity, lipid peroxidation profiles was also evident in the CYP+HT-treated group. We concluded that HT exhibited substantial antioxidant and anti-apoptotic impacts on the cerebral cortex cytomorphological alterations and apoptosis produced by CYP in cerebrum tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Amira Ebrahim Alsemeh, Mohamed A. M. AbdelRahman, Fatma A. Mahmoud, Eman Ramadan Abd El Fattah

Alsemeh

Abdel-Rahman

Mahmoud

et al. 2020

Journal of Medical Histology

View full text Add to dashboard Cite

show abstract

“…The present data are in agreement with those reported by El-Maghraby and Taha (2012) who found that the exposure of rats to Deltamethrin (DLM) induced significant decrease in glutathione-Stransferase (GST) enzyme activity and also GSH concentration. Das et al (2016) evaluated the protective role of zinc in attending cypermethrin induced haematological toxicity and oxidative stress in erythrocytes of male rat. They recorded reductions of glutathione content and antioxidant enzymes of rat erythrocytes.…”

Section: Effects Of Cypermthrin Vit E Zinc and Their Mixtures On Lmentioning

confidence: 99%

Toxic Effects of Administration of Cypermethrin, Vitamin E, Zinc and Their Mixtures on the Hormonal Levels of Thyroid, Kidney Functions and some Biochemical Parameters in Male Mice

Kamel

Reda²

2020

Journal of Applied Plant Protection

View full text Add to dashboard Cite

The present study investigates the effects of cypermethrin (CYP) on thyroid gland function, serum lipogram, kidney function, some liver enzymes and brain acetylcholinestrase (AchE) activity in male mice. Cypermethrin was administrated orally to mice at 9 mg/kg b.wt/day for 4 weeks (Dose period) followed by 2 weeks of ceasing treatments (recovery period). Male mice were randomly divided into five groups of eight each: a control (untreated check group) (1), CYP-treated group (2), CYP (9 mg/kg/day) +vitamin E (18 mg/kg/day) (3), CYP + zinc (20 mg/kg/day) (4) and CYP+E+zinc (5). Results revealed that triiodothyronine (T3) and thyroxine (T4) were significantly decreased in CYPgroup compared to control. In addition, the thyroid stimulating hormone (TSH) was also affected and recorded a significant increase by the treatments of CYP alone and CYP+vit E during the administration period. Also, significant increases of some biochemical parameters of kidney function (uric acid and creatinine) in serum were observed in CYPgroup compared to control. The administration of zinc with CYP group (4) improved all parameters studied (uric acid, creatinine, T3, T4 and TSH) as compared with control. The level of total lipid was significantly decreased, while triglycerides, total cholesterol and HDL-cholesterol were significantly increased by cypermethrin administration. The activity of liver and serum gamma glutamyl transferase (GGT) were significantly increased after CYP administration, while the results showed marked reduction of liver glutathione (GSH) concentration and the activity of glutathione-stransferase (GST). After cypermethrin administration with vitamin E, the activities of GGT, GST, and GSH level were significantly decreased compared to control. Moreover, inhibition percentage of brain acetylcholinestrase (AchE) activity was 62.98% by cypermethrin alone, but other groups (3), (4) and (5) had no effect on brain AchE activity. Results demonstrated the beneficial influence of vitamin E and zinc addition in combinations to reduce the harmful effects of cypermethrin.

show abstract

“…Although pyrethroids are rapidly replacing other insecticides due to relatively lower toxicity for mammals, the extensive and uncontrolled use have resulted in harmful effects (Arif et al, 2020). Many studies (El-Demerdash, 2007;Sadowska-Woda et al, 2010;Das et al, 2016;Arif et al, 2020) reported the induction of oxidative stress by pyrethroids such as lambda-cyhalothrin, bifenthrin, cypermethrin, and bioallethrin. Cypermethrin (CYP) a type II pyrethroid pesticide is used widely for pest control in agriculture, household, and garden either alone or in combination (AL-Eed, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Although their well-developed antioxidant defense system, RBCs can be oxidatively injured, as a result of exposure to toxic substances and environmental pollutants. Many studies have determined various structural changes in erythrocytes accompanied with biochemical changes as a result of exposure to different pyrethroids as fenvalerate, cypermethrin, lambda-cyhalothrin, bifenthrin, and bioallethrin (Prasanthi and Rajini, 2005;Sadowska-Woda et al, 2010;Das et al, 2016;Deeba et al, 2017;Arif et al, 2020). Oxidative stress influences and alters RBC shape (Bolotta et al, 2018), whose effective antioxidant mechanism, in the form of antioxidative enzymes such as CAT, SOD, and GST scavenges ROS to sustain membrane integrity (Quintana et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Effect of Cypermethrin on the Perturbations of Rat Erythrocytes: The Ameliorative Role of Carvone

Hamed

Mosallam

Abdel-Razik

2022

Alexandria Science Exchange Journal

View full text Add to dashboard Cite

Erythrocytes are a suitable model to study the membrane oxidative injury resulted by different xenobiotic. An attempt was made to study the antioxidant property of carvone (CAR), a monoterpene presents in herbs to mitigate toxicity of cypermethrin (CYP), a type II pyrethroid, in male rat erythrocytes in vitro. Erythrocytes were prepared and exposed to CYP (25-800 μM) alone and together with CAR (40 and 320 μM). Results showed superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities were significantly decreaseed. Lipid peroxidation (LPO) level, reduced glutathione (GSH) content, both of glutathione stransfarase (GST), and glutathione reductase (GR) activities were significantly increased in comparison of control value. Scanning electron microscope (SEM) supported the toxic effect of CYP which presented various degrees of alteration in shape and ruptured membranes. Pre-treatment of erythrocytes with CAR induced alleviation in all the tested parameters. It can be recommended that CYP induced oxidative stress in vitro in rat erythrocytes and supplementation with CAR ameliorated these effects.

show abstract

Ameliorative role of zinc on cypermethrin-induced changes in haematological parameters and oxidative stress biomarkers in rat erythrocytes

Cited by 13 publications

References 53 publications

Amira Ebrahim Alsemeh, Mohamed A. M. AbdelRahman, Fatma A. Mahmoud, Eman Ramadan Abd El Fattah

Amira Ebrahim Alsemeh, Mohamed A. M. AbdelRahman, Fatma A. Mahmoud, Eman Ramadan Abd El Fattah

Toxic Effects of Administration of Cypermethrin, Vitamin E, Zinc and Their Mixtures on the Hormonal Levels of Thyroid, Kidney Functions and some Biochemical Parameters in Male Mice

In Vitro Effect of Cypermethrin on the Perturbations of Rat Erythrocytes: The Ameliorative Role of Carvone

Contact Info

Product

Resources

About