Ameliorative role of trans-ferulic acid on induced oxidative toxicity of rooster semen by β-cyfluthrin during low temperature liquid storage

Abstract

Current study was designed to evaluate the effects of β-cyfluthrin, as a toxicant substance, and trans -ferulic acid ( trans - FA ), as a protective agent, on different parameters of rooster semen upon liquid storage. For this purpose, semen samples of roosters (Ross 308, n = 10, 32-wk-old) were collected twice a week. Good quality samples (≥70% progressive motion) were diluted, pooled and then divided for the purposes of the study. In the first experiment, motility of spermatozoa was evaluated following exposure to different concentrations of β-cyfluthrin (1, 2.5, 5, 10, 20, 40, and 80 µM) at 0, 24, and 48 h of storage. In the second experiment, constant doses of β-cyfluthrin (10 µM) alone or in combination with trans -FA (10, 25 mM) were assessed on motility and viability of spermatozoa at 0, 24, and 48 h time points. Moreover, amounts of malondialdehyde ( MDA ), total antioxidant capacity ( TAC ), total nitrate-nitrite, total hydroperoxide ( HPO ), and activity of superoxide dismutase ( SOD ) were evaluated in the homogenate of spermatozoa-diluent at studied time points. Results of the first experiment showed that amounts of β-cyfluthrin greater than 5 µM, significantly reduced the motility of spermatozoa at 24 and 48 h of storage ( P < 0.05). The second experiment demonstrated that, trans -FA especially at 10, 25 mM doses restored the motility and viability of spermatozoa compared to β-cyfluthrin treated group ( P < 0.05). Amounts of MDA (10, 25 mM), hydroperoxide (10, 25, and 50 mM), and nitrate-nitrite (10, 25, and 50 mM) were lower and TAC (10 and 25 mM) were greater in trans -FA + β-cyfluthrin treated groups compared to β-cyfluthrin alone treated samples ( P < 0.05). However, activity of SOD did not show significant changes by the treatment ( P > 0.05). It seems that trans -FA could ameliorate toxic effect of β-cyfluthrin via reduction of peroxidative (as evident by measurement of MDA) and nitrosative (as evident by measurement of nitrate-nitrite) reactions over cold preservation of rooster semen.

β-Cyfluthrin-Mediated Cytotoxicity of Cultured Rat Primary Hepatocytes Ameliorated by Cotreatment with Luteolin

“…Moreover, due to the high lipophilicity of β -cyfluthrin, it could easily penetrate via cell membranes and exert its detrimental effects within the cells [ 39 ]. Previous reports revealed that peroxidative reaction is one of the pathways of induced toxicity by pyrethroid insecticides over in vitro exposure [ 10 , 40 , 41 ]. According to the results of MTT (in the first and second experiments) and MDA levels, the current study confirmed that β -cyfluthrin induces oxidative toxicity in the primary culture of rat hepatocytes.…”

“…In accordance with our results, degenerative changes and reduced hepatocyte viability were indicated after exposure to cypermethrin or pyrrolidine alkaloids [ 46 , 47 ]. The reduction of hepatocyte viability in the group treated with β -cyfluthrin (alone) might be strongly related to the generation of free radicals, stimulation of peroxidative and nitrosative reactions, a significant increase in MDA and TNN levels, and ultimately reduction of enzymatic antioxidant amount [ 10 , 48 ]. Moreover, the results of the current study revealed the hepatoprotective role of luteolin against toxic doses of β -cyfluthrin.…”

“…Moreover, due to the high lipophilicity of β-cyfluthrin, it could easily penetrate via cell membranes and exert its detrimental effects within the cells [39]. Previous reports revealed that peroxidative reaction is one of the pathways of induced toxicity by pyrethroid insecticides over in vitro exposure [10,40,41].…”

“…Hepatocyte viability was assessed using 5-diphenyltetrazolium bromide (MTT) at 0, 24, and 48 h after exposure. The different stock solutions of β -cyfluthrin (80, 40, 20, 10, and 0 mM) dissolved in DMSO were prepared [ 10 ], and every well received an equal volume of DMSO-dissolved β -cyfluthrin (2 μ L in 1000 μ L medium) with different concentrations of β -cyfluthrin. A diluted sample was set as a negative control group at each run.…”

“…However, the toxicity of β-cy uthrin on di erent tissues and organs has been reported by researchers [4][5][6][7][8][9]. In this regard, oxidative and nitrosative e ects of β-cy uthrin during in vitro preservation of spermatozoa were also indicated [10]. Moreover, the stimulatory e ect of β-cy uthrin on the generation of reactive oxygen species (ROS) and ultimately related oxidative toxicity has been demonstrated in rat kidneys, aquatic organisms, and erythrocytes of rabbits [11][12][13].…”