Ameliorative Role of Melissa officinalis against Hepatorenal Toxicities of Organophosphorus Malathion in Male Rats

Abstract

It has been confirmed that organophosphorus compounds OP altered glucose homeostasis. Considerable experimental and clinical evidences have contributed the beneficial effects of antioxidant compounds in plant extract on hepatorenal toxicity. The aim of this study was to evaluate whether Melissa officinalis extract, was able to ameliorative hepatorenal injury induced by organophosphorus Malathion. Malathion at 27mg/kg bw was administered to rats alone or in combination with Melissa officinalis extract at 200mg/kg bw. Malathion decreases feed intake and body weight as well as liver and kidneys weight. Moreover, Malathion increases the levels of various serum marker enzymes AST, ALT and ALP. A significant increase in the level of serum urea, uric acid and creatinine in response to ML treatment. As well as ML induced increase in MDA in liver and kidneys while induced decreases in the activities of SOD, GPx and CAT. Co-administration of malathion with Melissa officinalis extract resulted in restoration of all tested parameter. These results may be due to the significant increase recorded in acetylcholine esterase (Ach E) activity invivo after co administration of malathion and Melissa officinalis extract. In histological study of liver and kidney, Malathion induced damage in liver and kidneys. However, MO administration to ML-treated animals resulted in overall improvement in liver and kidneys damage, emphasizing its antioxidant role. In light of the available data, it can deduce that ML-induced lipid peroxidation, oxidative stress, liver and kidneys damage rats, and conjoint supplementation of MO has resulted in pronounced ameliorating effect in all tested parameter and histological picture of liver and kidney. MOJ Toxicology Research ArticleOpen Access Ameliorative role of melissa officinalis against hepatorenal toxicities of organophosphorus malathion in male rats 104Copyright: ©2015 Sief et al. Citation:Sief MM, Khalil FA, Abou-Arab AA, et al. Ameliorative role of melissa officinalis against hepatorenal toxicities of organophosphorus malathion in male rats.

Ameliorative Effects of Vanillin Against Metribuzin-Induced Oxidative Stress and Toxicity in Rats

“…The activities of ALT, AST and ALP enzymes are the most sensitive biomarkers directly implicated in the extent of hepatic damage and toxicity [34]. In our findings, we demonstrated that Mtz administrated to rats provoked a marked elevation in serum AST, ALT and ALP activities which indicating hepatocellular damage as previously reported by Sief et al [30] and Khalil et al [35]. This elevation could potentially be attributed to the release of these enzymes from the cytoplasm into the blood circulation, indicating a necrosis and inflammatory reactions [34].…”

“…Also, Chiali et al [6] reported that metribuzin can alter plasma urea and creatinine levels due to renal failure. Besides, similar observations have been reported in previous study following exposure to other pesticides [29,30]. Many pesticides can cause some toxic and adverse effects on the kidney tissues [9].…”

“…Many pesticides can cause some toxic and adverse effects on the kidney tissues [9]. Therefore, the impairment in kidney function could probably occur via kidney oxidative damage [30].…”