Ameliorative role of diets fortified with Curcumin in a Drosophila melanogaster model of aluminum chloride-induced neurotoxicity

Spinacia oleracea (spinach) and Musa acuminata (banana) were chosen for the study, and aqueous extracts of spinach leaf extract (SLE) and banana peel extract (BPE) were prepared for the synthesis of iron nanoparticles (FeNPs), and their antibacterial potential against pathogenic bacteria Bacillus subtilis (MTTC 1133) and Escherichia coli (MTTC 62) was evaluated. In 10 minutes at 60°C, the color of the mixture (FeCl3+SLE) changed from light green to dark blackish-brown, and the color of the mix (FeCl3+BPE) changed from transparent yellow to dark black, confirming the synthesis of FeNPs from SLE and BPE, respectively. The UV-Vis spectra of spinach- and banana-derived FeNPs revealed two peaks ranging from 240 to 430 nm and multiple peaks at 240, 270, and 395 nm, respectively. FTIR spectroscopy was used to show different functional groups on BPE and SLE, and their role in FeNP synthesis was predicted. TEM micrographs showed that the particles were in nanoscale, ranging in size from 20 to 50 nm for BPE-derived FeNPs and 10 to 70 nm for SLE-derived FeNPs. The FeNP (BPE and SLE) XRD analysis revealed amorphism, with a weak iron characteristic peak, indicating noncrystallinity. The antibacterial potential of BPE- and SLE-FeNPs was investigated, and inhibition zones (mm) against B. subtilis ( 22.70 ± 0.4 ) and E. coli ( 20.45 ± 1.66 ) were observed, as well as SLE-FeNPs against B. subtilis ( 23.56 ± 1.00 ) and E. coli ( 20.33 ± 0.58 ). There were no significant differences in antibacterial activities between BPE-FeNPs and SLE-FeNPs. Positive controls were tetracycline and gentamicin, both standard antibiotics, at 5 μg/disk. SLE- and BPE-derived green FeNPs were also analysed in vivo of D. melanogaster life history traits, i.e., fecundity, hatchability, viability, and duration of development for toxicity evaluation. SLE- and BPE-derived green FeNPs at a concentration of 10 mg/L were fed flies compared to normal diet-fed flies (control sample), and no significant differences were observed between them. The findings suggest that FeNPs have a high antibacterial potential and could be used as antibacterial agents against pathogenic bacteria while being nontoxic in nature.

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“…This finding again confirms the nontoxic nature of FeNPs. Similar findings were found by other researchers [71][72][73].…”

Section: In Vivo Toxicity Evaluation Of Sle-and Bpe-derivedsupporting

confidence: 93%

Green Synthesis of Iron Nanoparticles from Spinach Leaf and Banana Peel Aqueous Extracts and Evaluation of Antibacterial Potential

Tyagi¹,

Gupta²,

Tyagi

et al. 2021

Journal of Nanomaterials

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“…For instance, in a study in which Drosophila Melanogaster was exposed to Al 3+ metal ion, it has been reported that antioxidant parameters such as catalase, glutathione S-transferase and glutathione decreased and free radical precursors such as NO and H 2 O 2 increased. The effect of this oxidative damage caused by Al 3+ ion was reported to be eliminated by the curcumin molecule depending on the dose (Oyetayo et al, 2020).…”

Section: Review Articlementioning

confidence: 99%

Curcumin's antioxidant effects on inflammatory diseases

Selen¹,

Çomaklı²

2021

Food Health

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There are similar inflammatory reasons behind non-contagious chronic diseases. The prevalence of these diseases increases everyday both in our country and around the world. That's why scientists have begun looking for strong antioxidants that could help prevent and treat such inflammatory diseases. Curcumin is one of those antioxidants. Curcumin is one of the components of turmeric, which belongs to the ginger family. Many studies showed that the curcuminoids in turmeric can be used to prevent and treat cardiovascular, autoimmune and endocrine diseases, cancer as well as various inflammatory diseases. With this study, we aim to interpret these recent studies conducted with curcumin.

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“…Fruit fly otherwise known as Drosophila melanogaster is an invertebrate (nonmammalian) model organism, now in use for experimental studies of human diseases, including AD (Oyetayo et al, 2020). This model organism has gained wide acceptance due to its many remarkable features and reasonable advantages it has over other model organisms.…”

Section: Introductionmentioning

confidence: 99%

“…The transgenic D. melanogaster genome used in this study is modified to express some human proteins; human β-secretase (BACE-1) and the human amyloid precursor protein (hAPP). Interestingly, the use of Drosophila in the laboratory is not impeded by ethical requirements hence, its increased use by researchers in the studies of human diseases including AD (Abolaji et al, 2020;Akinyemi et al, 2018;Liu et al, 2019;Oyetayo et al, 2020). This study was designed to evaluate the effect of dietary inclusion of fireweed leaf on behavioural patterns, memory indices, activities of cholinergic together, and monoaminergic enzymes in a fruit fly model of AD.…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary inclusion of Fireweed (Crassocephalum crepidioides) on behavioural patterns, memory indices, and activities of cholinergic and monoaminergic enzymes in a fruit fly (Drosophila melanogaster) model of Alzheimer's disease

et al. 2021

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Dietary supplementation of functional foods has been established for cognitive enhancement. Fireweed (Crassocephalum crepidioides) is a notable vegetable reported in folklore for its anti-neurodegenerative property, with paucity of information on its mechanism of action using a cell-based system. This study sought to evaluate the effect of fireweed leaf dietary inclusion (FDI) on some biochemical indices of neurodegeneration of a fruit fly (Drosophila melanogaster) model of Alzheimer's disease (AD). Transgenic (modeled for AD) and Harwich (the wild control) fruit fly strains were used, and were exposed separately to 0%, 0.31%, 0.63%, 1.25%, and 2.50% FDI for 14 days. Survival rate, locomotive performance, and short-term memory index of the flies were assessed. Thereafter, the flies' homogenates were prepared and used for the determination of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and monoamine oxidase (MAO), catalase, superoxide dismutase, and glutathione S-transferase activities, total thiol, reactive oxygen species (ROS), and thiobarbituric acid reactive species (TBARS) levels. The result revealed that AD flies treated with FDI had 70%-80% improved survival rate and behavioural performance. Furthermore, AChE, BChE, and MAO activities were significantly (p < 0.05) inhibited, while antioxidant status was also boosted in the AD flies comparable with the wild control flies. In conclusion, the modulation of cholinesterases and monoamine oxidase activities as well as oxidative stress biomarkers could be among the neuroprotective mechanisms of fireweed leaf. These effects could be attributed to the abundant polyphenols and dietary fibers present.Hence, its use in folklore for the management of neurological disorder is justified.

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Ameliorative role of diets fortified with Curcumin in a Drosophila melanogaster model of aluminum chloride-induced neurotoxicity

Cited by 19 publications

References 59 publications

Green Synthesis of Iron Nanoparticles from Spinach Leaf and Banana Peel Aqueous Extracts and Evaluation of Antibacterial Potential

Green Synthesis of Iron Nanoparticles from Spinach Leaf and Banana Peel Aqueous Extracts and Evaluation of Antibacterial Potential

Curcumin's antioxidant effects on inflammatory diseases

Effect of dietary inclusion of Fireweed (Crassocephalum crepidioides) on behavioural patterns, memory indices, and activities of cholinergic and monoaminergic enzymes in a fruit fly (Drosophila melanogaster) model of Alzheimer's disease

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