Ameliorative Potential of Aqueous Root Extract of Withania somnifera Against Paracetamol Induced Liver Damage in Mice

Malik, Tabarak; Pandey, Devendra Kumar; Dogra, Nitu

doi:10.5567/pharmacologia.2013.89.94

Cited by 14 publications

(19 citation statements)

References 8 publications

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“…It has been reported that WNA is the most stable and bioavailable withanolide under in vivo condition;[ 13 14 25 26 27 ] results of our previous studies are in conformity of this observation. [ 27 ] MDCK cells grown under the specified conditions have a narrow range of paracellular permeability (1 × 10 −6 to 1 × 10 −5 cm / s).…”

Section: Discussionsupporting

confidence: 89%

“…The biological activity of some individual withanolides has been evaluated in in vivo assay system. [ 8 9 10 11 12 13 14 15 ] However, as far as we are aware in vitro assay for evaluation of bioavailability of withanolides have not been carried out. Assessment of the bioavailability of a compound is a most important primary step in drug development.…”

Section: Discussionmentioning

confidence: 99%

“…[ 8 9 10 11 ] WN A is effective as a neurological, immunological and anti-stress agent. [ 12 13 14 ] WS IV and V play an important neuro-regenerative role. Thus in spinal cord injury WS IV and V improve hindlimb function and increase the myelin layer in the peripheral nervous system.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the bioavailability of major withanolides of Withania somnifera using an in vitro absorption model system

Devkar¹,

Kandhare²,

Sloley³

et al. 2015

J Adv Pharm Technol Res

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Withania somnifera (L.) Dunal, shows several pharmacological properties which are attributed mainly to the withanolides present in the root. The efficacy of medicinally active withanolides constituents depends on the absorption and transportation through the intestinal epithelium. We examined these characteristics by employing the Sino-Veda Madin-Darby canine kidney cells culture system, which under in vitro condition shows the absorption characteristics similar to the human intestinal epithelium. Thus, the aim of the present investigation was to assess the bioavailability of individual withanolides. Withanolides were diluted in Hank's buffered saline at a concentration of 2 μg/ml were tested for permeability studies carried out for 1 h duration. Permeability was measured in terms of efflux pump (Peff) in cm/s. Peff values of withanolide A (WN A), withanone (WNN), 1,2-deoxywithastramonolide (1,2 DWM), withanolide B (WN B), withanoside IV-V (WS IV-V), and withaferin A were 4.05 × 10−5, 2.06 × 10−5, 1.97 × 10−5, 1.80 × 10−5, 3.19 × 10−6, 3.03 × 10−6 and 3.30 × 10−7 respectively. In conclusion, the nonpolar and low molecular weight compounds (WN A, WNN, 1,2 DWM, and WN B) were highly permeable. As against this, the glycosylated and polar WS IV and WS V showed low permeability. Surprisingly and paradoxically, the highly biologically active withaferin A was completely impermeable, suggesting that further studies possibly using human epithelial colorectal adenocarcinoma (Caco-2) cells may be needed to delineate the absorption characteristics of withanolides, especially withaferin A.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the bioavailability of major withanolides of Withania somnifera using an in vitro absorption model system

Devkar¹,

Kandhare²,

Sloley³

et al. 2015

J Adv Pharm Technol Res

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“…The GSH depletion in PCMinduced toxicity may weaken the glutathione peroxidase activity, resulting in accumulation of H 2 O 2 which may further enhance the oxidative stress in PCM-induced rats (Malik et al 2013). However, the PPG supplementation was able to increase the GSH level not only in PCM-induced groups but also in the normal group.…”

Section: Discussionmentioning

confidence: 99%

Supplementation of Psidium guajava (Guava) Fruit Polysaccharide Attenuates Paracetamol-induced Liver Injury by Enhancing the Endogenous Antioxidant Activity

Alias¹,

Othman²,

Li³

et al. 2015

JSM

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“…Investigations have given numerous evidences, where W. somnifera at a dose 500 mg/kg significantly reduces the elevated biomarkers (aspartate aminotransferase, alanine transaminase, alkaline phosphatase, and Bilirubin) in experimental animals when exposed to hepatotoxic dose of paracetamol. It significantly reduces the lipid peroxidation, enhances glutathione content, catalase, glutathione reductase and glutathione peroxidase activity in liver (Malik et al, 2013;Sabina et al, 2013). In another study, W. somnifera has shown its hepatoprotective activity against gamma radiation induced toxicity in rodents, where 100 mg/kg dose of W. somnifera significantly decreases hepatic serum enzymes, levels of malondialdehyde, total nitrate/nitrite NO(x) and also heme oxygenase activity in liver.…”

Section: Hepatoprotective Activitymentioning

confidence: 99%

Chemistry and pharmacology of withania somnifera: An update

Kumar¹,

Dey²,

Hadimani³

et al. 2015

TANG [HUMANITAS MEDICINE]

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Withania somnifera (Ashwagandha) is an important Rasayana herb and widely considered as Indian ginseng in Ayurveda. In traditional system of Indian medicine, it is used as tonic to rejuvenate the body and increase longevity. In Ayurvedic preparations, various parts of the plant have been used to treat variety of ailments that affect the human health. However, dried roots of the plant are widely used for the treatment of nervous and sexual disorders. The major active chemical constituents of this plant are withanolides, which is responsible for its wide range of biological activities. Since the beginning of the 20 th century, a significant amount of research has been done and efforts are ongoing to further explore other bioactive constituents, and many pharmacological studies have been carried out to describe their disease preventing mechanisms. In this chapter, we have reviewed the chemistry and pharmacological basis of W. somnifera in various human ailments.

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Ameliorative Potential of Aqueous Root Extract of Withania somnifera Against Paracetamol Induced Liver Damage in Mice

Cited by 14 publications

References 8 publications

Evaluation of the bioavailability of major withanolides of Withania somnifera using an in vitro absorption model system

Evaluation of the bioavailability of major withanolides of Withania somnifera using an in vitro absorption model system

Supplementation of Psidium guajava (Guava) Fruit Polysaccharide Attenuates Paracetamol-induced Liver Injury by Enhancing the Endogenous Antioxidant Activity

Chemistry and pharmacology of withania somnifera: An update

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