Ameliorative effects of yokukansan on learning and memory deficits in olfactory bulbectomized mice

Yamada, Marina; Hayashida, Miki; Zhao, Qi; Shibahara, Naotoshi; Tanaka, Ken; Miyata, Takeshi; Matsumoto, Kinzo

doi:10.1016/j.jep.2011.04.010

Cited by 53 publications

(59 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, OBX has been used as one of the AD models since impairment of olfactory perceptual acuity is observed not only at the early stage of AD (14,15) but also in a transgenic AD model of mice over-expressing a mutant form of the human amyloid-bprecursor protein (16). In addition, OBX causes elevation of the Ab level in the brain (17) and the degeneration of septo-hippocampal cholinergic system in rodents (8,9,18), and thereby induces cognitive dysfunction (8 -10).…”

Section: Butea Superba-induced Amelioration Of Cognitive and Emotionamentioning

confidence: 99%

See 1 more Smart Citation

Butea superba–Induced Amelioration of Cognitive and Emotional Deficits in Olfactory Bulbectomized Mice and Putative Mechanisms Underlying Its Actions

et al. 2014

Self Cite

View full text Add to dashboard Cite

Abstract. This study investigated the effects of alcoholic extract of Butea superba (BS) on cognitive deficits and depression-related behavior using olfactory bulbectomized (OBX) mice and the underlying molecular mechanisms of its actions. OBX mice were treated daily with BS (100 and 300 mg/kg, p.o.) or reference drugs, tacrine (2.5 mg/kg, i.p.) and imipramine (10 mg/kg, i.p.) from day 3 after OBX. OBX impaired non-spatial and spatial cognitive performances, which were elucidated by the novel object recognition test and modified Y maze test, respectively. These deficits were attenuated by tacrine and BS but not imipramine. OBX animals exhibited depressionlike behavior in the tail suspension test in a manner reversible by imipramine and BS but not tacrine. OBX down-regulated phosphorylation of synaptic plasticity-related signaling proteins: NMDA receptor, AMPA receptor, calmodulin-dependent kinase II, and cyclic AMP-responsive element-binding protein. OBX also reduced choline acetyltransferase in the hippocampus. BS and tacrine reversed these neurochemical alterations. Moreover, BS inhibited ex vivo activity of acetylcholinesterase in the brain. These results indicate that BS ameliorates not only cognition dysfunction via normalizing synaptic plasticity-related signaling and facilitating central cholinergic systems but also depression-like behavior via a mechanism differing from that implicated in BS amelioration of cognitive function in OBX animals.

show abstract

Section: Butea Superba-induced Amelioration Of Cognitive and Emotionamentioning

confidence: 99%

“…This test is based on the tendency of mice to discriminate a familiar from a new object. This test was conducted as previously described (8,9,20) with minor modification. Briefly, mice were individually habituated to an observation box (50 × 50 × 40 cm) for 10 min on the day before the experiment.…”

Section: Novel Object Recognition Test (Ort)mentioning

confidence: 99%

Butea superba–Induced Amelioration of Cognitive and Emotional Deficits in Olfactory Bulbectomized Mice and Putative Mechanisms Underlying Its Actions

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…To identify the chemical constituents of KK, liquid column chromatography-mass spectrometry (LC-MS) analyses were performed with a Shimadzu LC-IT-TOF mass spectrometer equipped with an ESI interface ( Shimadzu, Kyoto) as previously described (5 …”

Section: Preparation and Chemical Analysis Of Kk Extractmentioning

confidence: 99%

“…Western blotting was performed by a slightly modified version of a method described previously (5,16,21). Briefly, tissue samples were taken from the hippocampus and proteins were extracted by homogenization in protein lysis buffer [50 mM Tris (pH 7.4), 150 mM NaCl, 0.5% sodium deoxycholate, 1% (v/v) NP-40, 0.1% (v/v) sodium dodecyl sulfate (SDS), 150 mM NaF, 8.12 μg/ml aprotinin, 2 mM sodium orthovanadate, 10 μg/ml leupeptin, and 2 mM phenylmethylsulfonyl fluoride] using TissueLyser ® (Qiagen, Osaka).…”

Section: Western Blot Analysismentioning

confidence: 99%

Diabetes-Induced Central Cholinergic Neuronal Loss and Cognitive Deficit Are Attenuated by Tacrine and a Chinese Herbal Prescription, Kangen-Karyu: Elucidation in Type 2 Diabetes db/db Mice

et al. 2011

Self Cite

View full text Add to dashboard Cite

Abstract.We investigated the effect of kangen-karyu (KK), a Chinese herbal prescription, on cognitive deficits and central cholinergic systems of type 2 diabetic db/db mice. Seven-week-old db/db (Y-db/db) mice received daily administration of test drugs during an experimental period of 12 weeks. At 18 weeks of age (O-db/db), the animals underwent the water maze test. Compared with age-matched control strain mice (O-m/m), vehicle-treated O-db/db mice showed impaired learning and memory performance. KK (100 -200 mg/kg per day) and the reference drug tacrine (THA: 2.5 mg/kg per day) ameliorated the performance of O-db/db mice without affecting their serum glucose level. O-db/db mice had lower levels of brain-derived neurotrophic factor (BDNF) mRNA and its protein in the brain than O-m/m mice. Expression levels of central cholinergic marker proteins in the hippocampus and the number of cholinergic cells in the medial septum and basal forebrain were also significantly lower in O-db/db than in O-m/m mice, whereas no significant differences in the expression levels of these factors and the cell number were found between Y-m/m and Y-db/db mice. KK and THA treatment significantly reversed the down-regulated levels of cholinergic markers, choline acetyltransferase-positive cell number, and BDNF expression in db/db mice. These findings suggest that KK as well as THA prevents diabetes-induced cognitive deficits by attenuating dysfunction of central cholinergic systems.

show abstract

“…The improvement of memory and BPSD-like behaviors with yokukansan (YKS) has been reported in OBX mice [27] and amyloid precursor protein transgenic mice [28]. However, the essential components of YKS and its mechanism to elucidate the improvement have not been defined.…”

Section: Discussionmentioning

confidence: 99%

Memory Improvement by Yokukansankachimpihange and Atractylenolide III in the Olfactory Bulbectomized Mice

Izumi¹,

Sasaki²,

Yabuki³

et al. 2016

AAD

View full text Add to dashboard Cite

Alzheimer's Disease (AD) shows cognitive dysfunction as core symptoms and Behavioral and Psychological Symptoms of Dementia (BPSD). Since acetylcholine nerve system derived from septum is collapsed in the AD patients, we have used Olfactory Bulbectomized (OBX) mice whose cholinergic system is largely impaired in the septum. Recently, Yokukansankachimpihange (YKH), a traditional Japanese Kampo medicine has used for BPSD in addition to improve cognitive dysfunction in AD patients. However the essential components for cognition and BPSD improvement and their mechanism are largely unknown. In present study, we found that Atractylenolide III (Aen-III), one of the components of YKH, improved cognitive deficits and

show abstract

Ameliorative effects of yokukansan on learning and memory deficits in olfactory bulbectomized mice

Cited by 53 publications

References 33 publications

Butea superba–Induced Amelioration of Cognitive and Emotional Deficits in Olfactory Bulbectomized Mice and Putative Mechanisms Underlying Its Actions

Butea superba–Induced Amelioration of Cognitive and Emotional Deficits in Olfactory Bulbectomized Mice and Putative Mechanisms Underlying Its Actions

Diabetes-Induced Central Cholinergic Neuronal Loss and Cognitive Deficit Are Attenuated by Tacrine and a Chinese Herbal Prescription, Kangen-Karyu: Elucidation in Type 2 Diabetes db/db Mice

Memory Improvement by Yokukansankachimpihange and Atractylenolide III in the Olfactory Bulbectomized Mice

Contact Info

Product

Resources

About