Ameliorative effects of 24-epibrassinolide and thiamine on excess cadmium-induced oxidative stress in Canola (<i>Brassica napus</i> L.) plants

Sanjari, Shima; Keramat, Batool; Nadernejad, Nazi; Mozafari, Hamid

doi:10.1080/17429145.2019.1637952

Cited by 23 publications

(10 citation statements)

References 56 publications

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“…As multiple, primarily beneficial effects on plant growth and development have been attributed to thiamin, there is a big incentive to elucidate the role of thiamin in plant physiology. These effects include increased tolerance to biotic (Bahuguna et al, 2012; Vinchesi et al., 2017 ; Hamada et al., 2018 ; Sathiyabama et al., 2019 ) and abiotic stresses such as salt/oxidative (Yee et al, 2016; Kaya et al., 2018 ; El-Shazoly et al., 2019 ), heavy metal ( Sanjari et al., 2019 ; Kaya and Aslan, 2020 ), and drought stress ( Li et al., 2016 ; Ghafar et al., 2019 ; Jabeen et al., 2020 ). The recently developed LC–MS/MS methodology to quantify the different thiamin vitamers as well as its biosynthesis intermediates ( Verstraete et al., 2020 ), together with engineered lines exhibiting altered B1 vitamer and/or biosynthesis intermediate content, could aid in pinpointing the exact metabolite provoking a given physiological effect.…”

Section: Discussionmentioning

confidence: 99%

Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants

et al. 2021

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Thiamin (or thiamine) is a water-soluble B-vitamin (B1), which is required, in the form of thiamin pyrophosphate (TPP), as an essential cofactor in crucial carbon metabolism reactions in all forms of life. To ensure adequate metabolic functioning, humans rely on a sufficient dietary supply of thiamin. Increasing thiamin levels in plants via metabolic engineering is a powerful strategy to alleviate vitamin B1 malnutrition and thus improve global human health. These engineering strategies rely on comprehensive knowledge of plant thiamin metabolism and its regulation. Here, multiple metabolic engineering strategies were examined in the model plant Arabidopsis thaliana. This was achieved by constitutive overexpression of the three biosynthesis genes responsible for B1 synthesis, HMP-P synthase (THIC), HET-P synthase (THI1) and HMP-P kinase/TMP pyrophosphorylase (TH1), either separate or in combination. By monitoring the levels of thiamin, its phosphorylated entities, and its biosynthetic intermediates, we gained insight into the effect of either strategy on thiamin biosynthesis. Moreover, expression analysis of thiamin biosynthesis genes showed the plant’s intriguing ability to respond to alterations in the pathway. Overall, we revealed the necessity to balance the pyrimidine and thiazole branches of thiamin biosynthesis and assessed its biosynthetic intermediates. Furthermore, the accumulation of non-phosphorylated intermediates demonstrated the inefficiency of endogenous thiamin salvage mechanisms. These results serve as guidelines in the development of novel thiamin metabolic engineering strategies.

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Section: Discussionmentioning

confidence: 99%

Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants

et al. 2021

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“…There seems to be a relationship between enhancing phenylalanine ammonia-lyase (PAL) and the total phenolic compound, and it has been reported that PAL is a key enzyme responsible for the activation of the synthesis of phenolic compounds under stress [57,58]. This phenomenon has been reported in some studies on the reduction of Cu and Cd toxicity [55,59]. Our results demonstrated that the application of TiO 2 NPs and EBL individually and in combination increased nonantioxidant activity (total phenolics, flavonols, and tocopherols) under Cu and Cd toxicity.…”

Section: Discussionmentioning

confidence: 92%

“…Additionally, TiO 2 reduces Chl degradation and stimulates Chl biosynthesis, which can promote photosynthesis by stabilizing chlorophylls and carotenoids [60]. Chlorophylls are the most abundant component of the chloroplast and play an efficient role in the rate of photosynthesis [59]. The role of EBL in enhancing the cell number and photosynthetic pigment content (Chl a, Chl b, and carotenoids) has been demonstrated in some studies [68,69].…”

Section: Discussionmentioning

confidence: 99%

Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes Pleioblastus pygmaeus Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and Translocation

et al. 2022

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The integrated application of nanoparticles and phytohormones was explored in this study as a potentially eco-friendly remediation strategy to mitigate heavy metal toxicity in a bamboo species (Pleioblastus pygmaeus) by utilizing titanium oxide nanoparticles (TiO2-NPs) and 24-epibrassinolide (EBL). Hence, an in vitro experiment was performed to evaluate the role of 100 µM TiO2 NPs and 10−8 M 24-epibrassinolide individually and in combination under 100 µM Cu and Cd in a completely randomized design using four replicates. Whereas 100 µM of Cu and Cd reduced antioxidant activity, photosynthetic capacity, plant tolerance, and ultimately plant growth, the co-application of 100 µM TiO2 NPs and 10−8 M EBL+ heavy metals (Cu and Cd) resulted in a significant increase in plant antioxidant activity (85%), nonenzymatic antioxidant activities (47%), photosynthetic pigments (43%), fluorescence parameters (68%), plant growth (39%), and plant tolerance (41%) and a significant reduction in the contents of malondialdehyde (45%), hydrogen peroxide (36%), superoxide radical (62%), and soluble protein (28%), as well as the percentage of electrolyte leakage (49%), relative to the control. Moreover, heavy metal accumulation and translocation were reduced by TiO2 NPs and EBL individually and in combination, which could improve bamboo plant tolerance.

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“…But foliar‐applied thiamin at the concentration of 50 and 100 mM significantly improved plant growth under stress as well as non‐stress conditions. Vitamins (thiamin) particularly of group B are the precursors of metabolic cofactors for enzymes and co‐enzymes, but they are commonly destroyed in plants upon exposure to abiotic stresses for short or long periods (Hanson et al 2016, Sanjari et al 2019). So, stress‐induced deficiency in the accumulation of vitamins is one of the primary symptoms for metabolic knock‐on under water deficiency (Hanson et al 2016, Sanjari et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Vitamins (thiamin) particularly of group B are the precursors of metabolic cofactors for enzymes and co‐enzymes, but they are commonly destroyed in plants upon exposure to abiotic stresses for short or long periods (Hanson et al 2016, Sanjari et al 2019). So, stress‐induced deficiency in the accumulation of vitamins is one of the primary symptoms for metabolic knock‐on under water deficiency (Hanson et al 2016, Sanjari et al 2019). In a previous study with white clover ( Trifolium repens L.), Ghafar et al (2019) reported that foliage spray of 50 mM thiamin improved plant growth under drought stress conditions.…”

Section: Discussionmentioning

confidence: 99%

Thiamin stimulates growth and secondary metabolites in turnip (Brassica rapa L.) leaf and root under drought stress

Jabeen

Akram

Ashraf

et al. 2020

Physiologia Plantarum

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Thiamin, an important member of the vitamin B family, is believed to play a significant role in mitigating environmental stresses including drought stress. In turnip, drought stress causes a reduced growth, biomass yield, pigment content, total phenolics and ascorbic acid (AsA), particularly at 50% field capacity (F.C.) in the two cultivars (cv) studied. However, a significant enhancement was observed in the contents of leaf proline, glycinebetaine (GB), malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ) and the activities of catalase (CAT) and superoxide dismutase (SOD) as well as root proline, GB, total phenolics, AsA, H 2 O 2 , MDA and the activities of peroxidase (POD) and SOD. However, foliar-applied thiamin significantly improved (particularly 100 mM) all the growth attributes, photosynthetic pigments, leaf and root osmoprotectants (GB and proline), AsA, total phenolics and the activities of enzymatic antioxidants such as SOD and POD as well as root CAT in both turnip cultivars under drought stress conditions. Foliar application of thiamin was effective in decreasing the leaf and root H 2 O 2 and MDA content in both cultivars particularly at 50% F.C. Thiamin-induced growth of both turnip cultivars, particularly of cv. Purple Top, was found to be associated with increased photosynthetic pigments, proline and GB contents and antioxidant capacity, but reduced levels of reactive oxygen species (ROS) under water deficit conditions. So, it is suggested that exogenous application of thiamin can be effective in improving drought tolerance of plants.

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Ameliorative effects of 24-epibrassinolide and thiamine on excess cadmium-induced oxidative stress in Canola (Brassica napus L.) plants

Abstract: View related articles View Crossmark data Citing articles: 3 View citing articles Ameliorative effects of 24-epibrassinolide and thiamine on excess cadmium-induced oxidative stress in Canola (Brassica napus L.

Cited by 23 publications

References 56 publications

Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants

Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants

Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes Pleioblastus pygmaeus Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and Translocation

Thiamin stimulates growth and secondary metabolites in turnip (Brassica rapa L.) leaf and root under drought stress

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